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sample_name:"Histone H3" AND xref_links:"chembl" AND author_name:"Ehara H"
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Paused elongation complex of mammalian RNA polymerase II with nucleosome (PEC2-nuc)

Release date: Feb. 5, 2025
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9j0n
Q-score: 0.316

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC1-nuc)

Release date: Feb. 5, 2025
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9j0o
Q-score: 0.313

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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Arrested elongation complex of mammalian RNA polymerase II with nucleosome (AEC2-nuc)

Release date: Feb. 5, 2025
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9j0p
Q-score: 0.311

Naganuma M, Kujirai T, Ehara H, Uejima T, Ito T, Goto M, Aoki M, Henmi M, Miyamoto-Kohno S, Shirouzu M, Kurumizaka H, Sekine S

Sci Adv (2025) [ DOI: doi:10.1126/sciadv.adu0577 ]

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RNA polymerase II elongation complex bound with Elf1, Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome

Release date: Nov. 29, 2023
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 8jh2
Q-score: 0.145

Akatsu M, Ehara H, Kujirai T, Fujita R, Ito T, Osumi K, Ogasawara M, Takizawa Y, Sekine SI, Kurumizaka H

J Biol Chem (2023) 299 pp. 105477-105477 [ DOI: doi:10.1016/j.jbc.2023.105477 Pubmed: 37981206 ]

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RNA polymerase II elongation complex containing 40 bp upstream DNA loop, stalled at SHL(-1) of the nucleosome

Release date: Nov. 29, 2023
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8jh3
Q-score: 0.255

Akatsu M, Ehara H, Kujirai T, Fujita R, Ito T, Osumi K, Ogasawara M, Takizawa Y, Sekine SI, Kurumizaka H

J Biol Chem (2023) 299 pp. 105477-105477 [ DOI: doi:10.1016/j.jbc.2023.105477 Pubmed: 37981206 ]

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RNA polymerase II elongation complex containing 60 bp upstream DNA loop, stalled at SHL(-1) of the nucleosome

Release date: Nov. 29, 2023
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8jh4
Q-score: 0.313

Akatsu M, Ehara H, Kujirai T, Fujita R, Ito T, Osumi K, Ogasawara M, Takizawa Y, Sekine SI, Kurumizaka H

J Biol Chem (2023) 299 pp. 105477-105477 [ DOI: doi:10.1016/j.jbc.2023.105477 Pubmed: 37981206 ]

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RNA polymerase II elongation complex bound with Elf1 and Spt4/5, stalled at SHL(-4) of the nucleosome

Release date: July 5, 2023
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7wbv
Q-score: 0.267

Osumi K, Kujirai T, Ehara H, Ogasawara M, Kinoshita C, Saotome M, Kagawa W, Sekine SI, Takizawa Y, Kurumizaka H

J Mol Biol (2023) 435 pp. 168130-168130 [ DOI: doi:10.1016/j.jmb.2023.168130 Pubmed: 37120012 ]

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RNA polymerase II elongation complex bound with Elf1 and Spt4/5, stalled at SHL(-3.5) of the nucleosome

Release date: July 5, 2023
Resolution: 7.1 Å
EM Method: Single-particle
Fitted PDBs: 7wbw
Q-score: 0.137

Osumi K, Kujirai T, Ehara H, Ogasawara M, Kinoshita C, Saotome M, Kagawa W, Sekine SI, Takizawa Y, Kurumizaka H

J Mol Biol (2023) 435 pp. 168130-168130 [ DOI: doi:10.1016/j.jmb.2023.168130 Pubmed: 37120012 ]

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RNA polymerase II elongation complex bound with Elf1 and Spt4/5, stalled at SHL(-3) of the nucleosome

Release date: July 5, 2023
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7wbx
Q-score: 0.298

Osumi K, Kujirai T, Ehara H, Ogasawara M, Kinoshita C, Saotome M, Kagawa W, Sekine SI, Takizawa Y, Kurumizaka H

J Mol Biol (2023) 435 pp. 168130-168130 [ DOI: doi:10.1016/j.jmb.2023.168130 Pubmed: 37120012 ]

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RNA polymerase II elongation complex bound with Rad26 and Elf1, stalled at SHL(-3.5) of the nucleosome

Release date: July 5, 2023
Resolution: 6.95 Å
EM Method: Single-particle
Fitted PDBs: 8he5
Q-score: 0.105

Osumi K, Kujirai T, Ehara H, Ogasawara M, Kinoshita C, Saotome M, Kagawa W, Sekine SI, Takizawa Y, Kurumizaka H

J Mol Biol (2023) 435 pp. 168130-168130 [ DOI: doi:10.1016/j.jmb.2023.168130 Pubmed: 37120012 ]

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