Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ssa
Q-score: 0.449
Donovan BT, Chen H, Eek P, Meng Z, Jipa C, Tan S, Bai L, Poirier MG
Mol Cell (2023) 83 pp. 1251-1263.e6 [ DOI: doi:10.1016/j.molcel.2023.03.006 Pubmed: 36996811 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2a.1 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Centromere-binding protein 1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (137-mer) (46 kDa, DNA from synthetic construct)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h3.2, histone h4 (Complex from Xenopus laevis)
- Dna (137-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a.1, histone h2b.1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (Complex from synthetic construct)
- Pioneer factor cbf1 in complex with the nucleosome (286 kDa, Complex)
Structure of VRK1 C-terminal tail bound to nucleosome core particle
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7tan
Q-score: 0.56
Budziszewski GR, Zhao Y, Spangler CJ, Kedziora KM, Williams MR, Azzam DN, Skrajna A, Koyama Y, Cesmat AP, Simmons HC, Arteaga EC, Strauss JD, Kireev D, McGinty RK
Nucleic Acids Res (2022) 50 pp. 4355-4371 [ Pubmed: 35390161 DOI: doi:10.1093/nar/gkac198 ]
- Serine/threonine-protein kinase vrk1 (45 kDa, Protein from Homo sapiens)
- Structure of vrk1 c-terminal tail bound to nucleosome core particle (Complex)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Histone h3.2, histone h4, histone h2a type 1, histone h2b type 1-c/e/f/g/i, serine/threonine-protein kinase vrk1 (Complex from Homo sapiens)
- Widom 601 dna (185-mer) (57 kDa, DNA from synthetic construct)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Histone h2a type 1 (13 kDa, Protein from Homo sapiens)
- Widom 601 dna (185-mer) (56 kDa, DNA from synthetic construct)
Uncrosslinked VRK1-nucleosome complex
Budziszewski GR, Zhao Y, Spangler CJ, Kedziora KM, Williams MR, Azzam DN, Skrajna A, Koyama Y, Cesmat AP, Simmons HC, Arteaga EC, Strauss JD, Kireev D, McGinty RK
Nucleic Acids Res (2022) 50 pp. 4355-4371 [ Pubmed: 35390161 DOI: doi:10.1093/nar/gkac198 ]
- Histone h3.2 (Protein from Homo sapiens)
- Structure of vrk1 c-terminal tail bound to nucleosome core particle (Complex)
- Widom 601 dna (185-mer) (DNA from synthetic construct)
- Vaccinia-related kinase 1 (Protein from Homo sapiens)
- Histone h3.2, histone h4, histone h2a type 1, histone h2b type 1-c/e/f/g/i, serine/threonine-protein kinase vrk1 (Complex from Homo sapiens)
- Histone h2a (Protein from Homo sapiens)
- Histone h4 (Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Histone h2b (Protein from Homo sapiens)