Cryo-EM structure of human DICER-pre-miRNA in a dicing state
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7xw2
Q-score: 0.444
Lee YY, Lee H, Kim H, Kim VN, Roh SH
Nature (2023) 615 pp. 331-338 [ Pubmed: 36813958 DOI: doi:10.1038/s41586-023-05723-3 ]
- Calcium ion (40 Da, Ligand)
- Pre-mirna (Complex)
- Rna (73-mer) (23 kDa, RNA from Homo sapiens)
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
- Human dicer (Complex from Homo sapiens)
- Protein-rna complex of human dicer with pre-mirna (Complex)
Cryo-EM structure of an apo-form of human DICER
Resolution: 4.04 Å
EM Method: Single-particle
Fitted PDBs: 7xw3
Q-score: 0.285
Lee YY, Lee H, Kim H, Kim VN, Roh SH
Nature (2023) 615 pp. 331-338 [ Pubmed: 36813958 DOI: doi:10.1038/s41586-023-05723-3 ]
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
- Monomeric form of human dicer (Complex from Homo sapiens)
Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 5zak
Q-score: 0.25
Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang QC, Wang HW
Cell (2018) 173 pp. 1191-1203.e12 [ Pubmed: 29706542 DOI: doi:10.1016/j.cell.2018.03.080 ]
- Dicer and trbp (250 kDa, Complex from Homo sapiens)
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
- Human dicer and trbp complex (Complex from Homo sapiens)
- Risc-loading complex subunit tarbp2 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 5zal
Q-score: 0.216
Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang QC, Wang HW
Cell (2018) 173 pp. 1191-1203.e12 [ Pubmed: 29706542 DOI: doi:10.1016/j.cell.2018.03.080 ]
- Rna (73-mer) (23 kDa, RNA from Homo sapiens)
- Risc-loading complex subunit tarbp2 (39 kDa, Protein from Homo sapiens)
- Dicer and trbp (270 kDa, Complex from Homo sapiens)
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
- Human dicer-trbp with pre-let-7 complex class i (Complex from Homo sapiens)
Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 5zam
Q-score: 0.215
Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang QC, Wang HW
Cell (2018) 173 pp. 1191-1203.e12 [ Pubmed: 29706542 DOI: doi:10.1016/j.cell.2018.03.080 ]
- Rna (73-mer) (23 kDa, RNA from Homo sapiens)
- Human dicer-trbp with pre-let-7 complex class ii (Complex from Homo sapiens)
- Risc-loading complex subunit tarbp2 (39 kDa, Protein from Homo sapiens)
- Dicer and trbp (270 kDa, Complex from Homo sapiens)
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
Substrate-specific structural rearrangements of human Dicer
Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA, Wang HW
Nat Struct Mol Biol (2013) 20 pp. 662-670 [ Pubmed: 23624860 DOI: doi:10.1038/nsmb.2564 ]
- Human dicer (220 kDa, Sample)
- Endoribonuclease dicer (220 kDa, Protein from Homo sapiens)
Substrate-specific structural rearrangements of human Dicer
Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA, Wang HW
Nat Struct Mol Biol (2013) 20 pp. 662-670 [ Pubmed: 23624860 DOI: doi:10.1038/nsmb.2564 ]
- Human dicer in complex with a 35 bp pre-sirna (250 kDa, Sample)
- 35 bp pre-sirna (24 kDa, RNA from Homo sapiens)
- Endoribonuclease dicer (220 kDa, Protein from Homo sapiens)
Substrate-specific structural rearrangements of human Dicer
Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA, Wang HW
Nat Struct Mol Biol (2013) 20 pp. 662-670 [ Pubmed: 23624860 DOI: doi:10.1038/nsmb.2564 ]
- Human dicer in complex with pre-let7 (250 kDa, Sample)
- Pre-let7 (24 kDa, Macromolecule from Homo sapiens)
- Endoribonuclease dicer (220 kDa, Protein from Homo sapiens)
Substrate-specific structural rearrangements of human Dicer
Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA, Wang HW
Nat Struct Mol Biol (2013) 20 pp. 662-670 [ Pubmed: 23624860 DOI: doi:10.1038/nsmb.2564 ]
- Interferon-inducible double stranded rna-dependent protein kinase activator a (34 kDa, Protein from Homo sapiens)
- Human dicer-pact heterodimer in closed conformation (260 kDa, Sample)
- Endoribonuclease dicer (220 kDa, Protein from Homo sapiens)
Substrate-specific structural rearrangements of human Dicer
Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA, Wang HW
Nat Struct Mol Biol (2013) 20 pp. 662-670 [ Pubmed: 23624860 DOI: doi:10.1038/nsmb.2564 ]