60S ribosome biogenesis intermediate (Dbp10 pre-catalytic structure - Overall map)
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8v83
Q-score: 0.53
Cruz VE, Weirich CS, Peddada N, Erzberger JP
Nat Commun (2024) 15 pp. 3296-3296 [ Pubmed: 38632236 DOI: doi:10.1038/s41467-024-47616-7 ]
- Ribosomal rna-processing protein 14 (50 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome subunit biogenesis protein nip7 (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el15a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Protein mak16 (35 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein ssf1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul1a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal rna-processing protein 15 (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (91 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome biogenesis intermediate (Complex from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el20a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal rna-processing protein 1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Saccharomyces cerevisiae s288c 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul6a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Ribosome production factor 1 (35 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Saccharomyces cerevisiae s288c 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Saccharomyces cerevisiae its-2 (74 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el8a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar protein 16 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal subunit assembly/export protein loc1 (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul13a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (cytosine(2870)-c(5))-methyltransferase (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
60S ribosome biogenesis intermediate (Dbp10 catalytic structure - Overall map)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8v84
Q-score: 0.461
Cruz VE, Weirich CS, Peddada N, Erzberger JP
Nat Commun (2024) 15 pp. 3296-3296 [ Pubmed: 38632236 DOI: doi:10.1038/s41467-024-47616-7 ]
- 60s ribosomal subunit assembly/export protein loc1 (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar protein 16 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Its2 rna (74 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul1a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Protein mak16 (35 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el15a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el8a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome production factor 1 (35 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Saccharomyces cerevisiae 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase dbp10 (113 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal rna-processing protein 14 (50 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul13a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal rna-processing protein 15 (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741-AV16)
- Ribosome biogenesis protein nsa1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (91 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el20a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal rna-processing protein 1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ribosome biogenesis protein ssf1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome subunit biogenesis protein nip7 (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul6a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Saccharomyces cerevisiae 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (cytosine(2870)-c(5))-methyltransferase (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome biogenesis intermediate (Complex from Saccharomyces cerevisiae BY4741)
- Magnesium ion (24 Da, Ligand)
60S ribosome biogenesis intermediate (Dbp10 post-catalytic structure - Overall map)
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8v87
Q-score: 0.519
Cruz VE, Weirich CS, Peddada N, Erzberger JP
Nat Commun (2024) 15 pp. 3296-3296 [ Pubmed: 38632236 DOI: doi:10.1038/s41467-024-47616-7 ]
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal subunit assembly/export protein loc1 (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar protein 16 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Its2 rna (74 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Potassium ion (39 Da, Ligand)
- Large ribosomal subunit protein ul1a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ytm1 isoform 1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el8a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el15a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 27s pre-rrna (guanosine(2922)-2'-o)-methyltransferase (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase dbp10 (113 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex-associated protein 3 (75 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul13a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex protein 2 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (91 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein el20a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 60s ribosome subunit biogenesis protein nip7 (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Large ribosomal subunit protein ul6a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (cytosine(2870)-c(5))-methyltransferase (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome biogenesis intermediate (Complex from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Magnesium ion (24 Da, Ligand)
Chaetomium thermophilum pre-60S State 5 - pre-5S rotation - L1 inward - composite structure
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 8pvk
Q-score: 0.606
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ Pubmed: 37921038 DOI: doi:10.15252/embr.202357984 ]
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- Ribosomal protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 5 - pre-5s rotation - l1 inward - composite structure (Complex)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Utp30 (43 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Chaetomium thermophilum pre-60S State 7 - pre-5S rotation lacking Utp30/ITS2 - composite structure
Resolution: 2.19 Å
EM Method: Single-particle
Fitted PDBs: 8pvl
Q-score: 0.662
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ Pubmed: 37921038 DOI: doi:10.15252/embr.202357984 ]
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 7 - pre-5s rotation lacking utp30/its2 - composite structure (Complex)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ Pubmed: 37921038 DOI: doi:10.15252/embr.202357984 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ Pubmed: 37921038 DOI: doi:10.15252/embr.202357984 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Chaetomium thermophilum pre-60S State 7 - pre-5S rotation lacking Utp30/ITS2 - consensus refinement
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ Pubmed: 37921038 DOI: doi:10.15252/embr.202357984 ]
Chaetomium thermophilum pre-60S State 6 - pre-5S rotation - L1 intermediate - composite structure
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8pv1
Q-score: 0.618
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ Pubmed: 37921038 DOI: doi:10.15252/embr.202357984 ]
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 6 - pre-5s rotation - l1 intermediate - composite structure (Complex)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Utp30 (43 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)