Chaetomium thermophilum pre-60S State 5 - pre-5S rotation - L1 inward - composite structure
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 8pvk
Q-score: 0.606
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 5 - pre-5s rotation - l1 inward - composite structure (Complex)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Utp30 (43 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Chaetomium thermophilum pre-60S State 7 - pre-5S rotation lacking Utp30/ITS2 - composite structure
Resolution: 2.19 Å
EM Method: Single-particle
Fitted PDBs: 8pvl
Q-score: 0.662
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 7 - pre-5s rotation lacking utp30/its2 - composite structure (Complex)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Chaetomium thermophilum pre-60S State 7 - pre-5S rotation lacking Utp30/ITS2 - consensus refinement
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Chaetomium thermophilum pre-60S State 6 - pre-5S rotation - L1 intermediate - composite structure
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8pv1
Q-score: 0.618
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 6 - pre-5s rotation - l1 intermediate - composite structure (Complex)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Utp30 (43 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Chaetomium thermophilum pre-60S State 9 - pre-5S rotation - immature H68/H69 - composite structure
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8pv3
Q-score: 0.573
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 9 - pre-5s rotation - immature h68/h69 - composite structure (Complex)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Utp30 (43 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Chaetomium thermophilum pre-60S State 2 - pre-5S rotation with Rix1 complex - composite structure
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8pv4
Q-score: 0.535
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein rix1 (84 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein ipi1 (37 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein ipi3 (47 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 2 - pre-5s rotation with rix1 complex - composite structure (Complex)
- Protein sda1 (86 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Utp30 (43 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8pv6
Q-score: 0.547
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein rix1 (84 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l29 (7 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein ipi1 (37 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein ipi3 (47 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Protein sda1 (86 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Chaetomium thermophilum pre-60s state 3 - post-5s rotation with rix1 complex with foot - composite structure (Complex)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)