Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8ssz
Q-score: 0.537
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- Neuronal acetylcholine receptor subunit beta-2 (55 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- N-octane (114 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
- A complex of three fab fragments with the 2alpha3beta stoichiometry of the full-length human alpha4beta2 nicotinic receptor bound to acetylcholine and calcium (Complex from Homo sapiens)
- Neuronal acetylcholine receptor subunit alpha-4 (67 kDa, Protein from Homo sapiens)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8st0
Q-score: 0.567
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- Neuronal acetylcholine receptor subunit beta-2 (55 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Water (18 Da, Ligand)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- A complex of three fab fragments with the 2alpha3beta stoichiometry of the full-length human alpha4beta2 nicotinic receptor bound to acetylcholine (Complex from Homo sapiens)
- N-octane (114 Da, Ligand)
- Neuronal acetylcholine receptor subunit alpha-4 (67 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8st1
Q-score: 0.496
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
- A complex of two fab fragments with the 3alpha2beta stoichiometry of human alpha4beta2 nicotinic receptor bound to acetylcholine and calcium (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8st2
Q-score: 0.545
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- A complex of two fab fragments with the 3alpha2beta stoichiometry of human alpha4beta2 nicotinic receptor bound to acetylcholine (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8st3
Q-score: 0.533
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- A complex of three fab fragments with the 2alpha3beta stoichiometry of human alpha4beta2 nicotinic receptor bound to acetylcholine and calcium (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Resolution: 2.35 Å
EM Method: Single-particle
Fitted PDBs: 8st4
Q-score: 0.597
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
- A complex of three fab fragments with the 2alpha3beta stoichiometry of human alpha4beta2 nicotinic receptor bound to acetylcholine (Complex from Homo sapiens)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Structure of the 2alpha3beta stiochiometry of the human Alpha4Beta2 nicotinic receptor
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6cnj
Q-score: 0.38
Walsh RM, Roh SH, Gharpure A, Morales-Perez CL, Teng J, Hibbs RE
Nature (2018) 557 pp. 261-265 [ DOI: doi:10.1038/s41586-018-0081-7 Pubmed: 29720657 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
- (s)-3-(1-methylpyrrolidin-2-yl)pyridine (162 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Complex of three fab fragments with the 2alpha3beta stoichiometry of the human alpha4beta2 nicotinic receptor (344 kDa, Complex from Homo sapiens)
Structure of the 3alpha2beta stiochiometry of the human Alpha4Beta2 nicotinic receptor
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6cnk
Q-score: 0.377
Walsh RM, Roh SH, Gharpure A, Morales-Perez CL, Teng J, Hibbs RE
Nature (2018) 557 pp. 261-265 [ DOI: doi:10.1038/s41586-018-0081-7 Pubmed: 29720657 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
- (s)-3-(1-methylpyrrolidin-2-yl)pyridine (162 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Complex of two fab fragments with the 3alpha2beta stoichiometry of the human alpha4beta2 nicotinic receptor (294 kDa, Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)