MVV cleaved synaptic complex (CSC) intasome at 4.5 A resolution (re-refined)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7zpp
Q-score: 0.097
Ballandras-Colas A, Maskell DP, Serrao E, Locke J, Swuec P, Jonsson SR, Kotecha A, Cook NJ, Pye VE, Taylor IA, Andresdottir V, Engelman AN, Costa A, Cherepanov P
Science (2017) 355 pp. 93-95 [ Pubmed: 28059770 DOI: doi:10.1126/science.aah7002 ]
- Intergrase (Complex from Visna/maedi virus EV1 KV1772)
- Vdna, non-transferred strand (6 kDa, DNA from Visna lentivirus (strain 1514))
- Vdna, transferred strand (5 kDa, DNA from Visna-maedi virus)
- Maedi-visna virus (mvv) intasome (542 kDa, Complex)
- Vdna (Complex from Visna-maedi virus)
- Integrase (32 kDa, Protein from Visna/maedi virus EV1 KV1772)
PFV intasome - nucleosome strand transfer complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6rny
Q-score: 0.349
Wilson MD, Renault L, Maskell DP, Ghoneim M, Pye VE, Nans A, Rueda DS, Cherepanov P, Costa A
Nat Commun (2019) 10 pp. 4189-4189 [ DOI: doi:10.1038/s41467-019-12007-w Pubmed: 31519882 ]
- Human dna (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2a type 1 (14 kDa, Protein from Homo sapiens)
- Dna (128-mer) (39 kDa, DNA from Homo sapiens)
- Dna (108-mer) (33 kDa, DNA from Homo sapiens)
- Nucleosome (Complex from Homo sapiens)
- Dna (53-mer) (16 kDa, DNA from Homo sapiens)
- Dna (5'-d(*ap*tp*tp*gp*tp*cp*ap*tp*gp*gp*ap*ap*tp*tp*tp*cp*gp*c)-3') (5 kDa, DNA from Pyrobaculum filamentous virus 1)
- Dna (33-mer) (10 kDa, DNA from Homo sapiens)
- Integrase (Complex from Human spumaretrovirus)
- Dna (Complex from Pyrobaculum filamentous virus 1)
- Nucleosome core particle bound by pfv intasome post in the post catalytic state (399 kDa, Complex)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Integrase (44 kDa, Protein from Human spumaretrovirus)
- Histone h3.3 (15 kDa, Protein from Homo sapiens)
Cryo-EM reconstruction of the maedi-visna virus (MVV) strand transfer complex
Resolution: 8.2 Å
EM Method: Single-particle
Fitted PDBs: 5m0r
Q-score: 0.098
Ballandras-Colas A, Maskell DP, Serrao E, Locke J, Swuec P, Jonsson SR, Kotecha A, Cook NJ, Pye VE, Taylor IA, Andresdottir V, Engelman AN, Costa A, Cherepanov P
Science (2017) 355 pp. 93-95 [ Pubmed: 28059770 DOI: doi:10.1126/science.aah7002 ]
- Nucleic acid (Complex from synthetic construct)
- Integrase (32 kDa, Protein from Maedi visna virus (strain KV1772))
- Intergrase (Complex from Maedi visna virus (strain KV1772))
- Vdna-tdna, transferred strand, joined to a model tdna (15 kDa, DNA from Visna lentivirus (strain 1514))
- Mvv strand transfer complex (Complex)
- Vdna, non-transfered strand (6 kDa, DNA from Visna lentivirus (strain 1514))
- Nucleic acid (Complex from Visna lentivirus (strain 1514))
- Tdna (7 kDa, DNA from synthetic construct)