Curved structure of mPIEZO1-S2472E
Liu S, Yang X, Chen X, Zhang X, Jiang J, Yuan J, Liu W, Wang L, Zhou H, Wu K, Tian B, Li X, Xiao B
Neuron (2025) 113 pp. 590- [ Pubmed: 39719701 DOI: doi:10.1016/j.neuron.2024.11.020 ]
- S2472e-curved structure (Complex from Mus musculus)
- Piezo-type mechanosensitive ion channel component 1 (292 kDa, Protein from Mus musculus)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- (9r,11s)-9-({[(1s)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol (767 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
Flattened structure of mPIEZO1-S2472E
Liu S, Yang X, Chen X, Zhang X, Jiang J, Yuan J, Liu W, Wang L, Zhou H, Wu K, Tian B, Li X, Xiao B
Neuron (2025) 113 pp. 590- [ Pubmed: 39719701 DOI: doi:10.1016/j.neuron.2024.11.020 ]
- Piezo-type mechanosensitive ion channel component 1 (292 kDa, Protein from Mus musculus)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- (9r,11s)-9-({[(1s)-1-hydroxyhexadecyl]oxy}methyl)-2,2-dimethyl-5,7,10-trioxa-2lambda~5~-aza-6lambda~5~-phosphaoctacosane-6,6,11-triol (767 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- S2472e-intermediate structure (Complex from Mus musculus)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8j7f
Q-score: 0.555
Chen H, Xia Z, Dong J, Huang B, Zhang J, Zhou F, Yan R, Shi Y, Gong J, Jiang J, Huang Z, Jiang D
Nat Commun (2024) 15 pp. 3691-3691 [ Pubmed: 38693179 DOI: doi:10.1038/s41467-024-48125-3 ]
- Ion channel complex (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Ion channel,voltage dependent ion channel,green fluorescent protein (fragment),voltage dependent ion channel,green fluorescent protein (fragment),ion transport domain-containing protein (33 kDa, Protein from Aequorea victoria)
- Ile-ala-ala-ile-his-asn-ala-arg-arg-lys-lys-arg-glu-ala-ala-ala-ala-his-lys-ala (2 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8j7m
Q-score: 0.53
Chen H, Xia Z, Dong J, Huang B, Zhang J, Zhou F, Yan R, Shi Y, Gong J, Jiang J, Huang Z, Jiang D
Nat Commun (2024) 15 pp. 3691-3691 [ Pubmed: 38693179 DOI: doi:10.1038/s41467-024-48125-3 ]
- Cholesterol (386 Da, Ligand)
- Ion channel (Complex from Homo sapiens)
- Ion channel,voltage dependent ion channel,green fluorescent protein (fragment),voltage dependent ion channel,green fluorescent protein (fragment),voltage dependent ion channel,green fluorescent protein (fragment) (90 kDa, Protein from Aequorea victoria)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8j7h
Q-score: 0.448
Chen H, Xia Z, Dong J, Huang B, Zhang J, Zhou F, Yan R, Shi Y, Gong J, Jiang J, Huang Z, Jiang D
Nat Commun (2024) 15 pp. 3691-3691 [ Pubmed: 38693179 DOI: doi:10.1038/s41467-024-48125-3 ]
- Ion channel complex (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Ile-ala-ala-ile-his-asn-ala-arg-arg-lys-lys-arg-glu-ala-ala-ala-ala-his-lys-ala (2 kDa, Protein from Homo sapiens)
- Ion channel (91 kDa, Protein from Homo sapiens)
ELIC5 with cysteamine in 2:1:1 POPC:POPE:POPG nanodisc in open conformation
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8vuw
Q-score: 0.538
Petroff JT, Dietzen NM, Santiago-McRae E, Deng B, Washington MS, Chen LJ, Trent Moreland K, Deng Z, Rau M, Fitzpatrick JAJ, Yuan P, Joseph TT, Henin J, Brannigan G, Cheng WWL
Nat Commun (2022) 13 pp. 7017- [ Pubmed: 36385237 DOI: doi:10.1038/s41467-022-34813-5 ]
- 2-amino-ethanethiol (77 Da, Ligand)
- Erwinia chrysanthemi ligand-gated ion channel (37 kDa, Protein from Dickeya chrysanthemi)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Elic5 (p254g/c300s/v261y/g319f/i320f) with cysteamine in 2:1:1 popc:pope:popg nanodisc (Complex from Dickeya chrysanthemi)
Cryo-EM structure of mouse Piezo1-MDFIC complex (Masked refinement of the cap domain)