Closed conformation of the pentameric ligand-gated ion channel, DeCLIC at pH 5 with 10 mM Ca2+
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9ftg
Q-score: 0.524
Open conformation of the pentameric ligand-gated ion channel, DeCLIC at pH 5 with 10mM EDTA
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9fti
Q-score: 0.494
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8j7h
Q-score: 0.448
Chen H, Xia Z, Dong J, Huang B, Zhang J, Zhou F, Yan R, Shi Y, Gong J, Jiang J, Huang Z, Jiang D
Nat Commun (2024) 15 pp. 3691-3691 [ Pubmed: 38693179 DOI: doi:10.1038/s41467-024-48125-3 ]
- Ion channel complex (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Ile-ala-ala-ile-his-asn-ala-arg-arg-lys-lys-arg-glu-ala-ala-ala-ala-his-lys-ala (2 kDa, Protein from Homo sapiens)
- Ion channel (91 kDa, Protein from Homo sapiens)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in intermediate state
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 8wcq
Q-score: 0.378
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
- Chloride ion (35 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
Cryo-EM structure of nanodisc (asolectin) reconstituted GLIC at pH 7.5
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 8i41
Q-score: 0.467
Bharambe N, Li Z, Seiferth D, Balakrishna AM, Biggin PC, Basak S
Nat Commun (2024) 15 pp. 2967-2967 [ DOI: doi:10.1038/s41467-024-47370-w Pubmed: 38580666 ]
- Pentameric ligand-gated ion channel (Complex from Gloeobacter violaceus)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Proton-gated ion channel (35 kDa, Protein from Gloeobacter violaceus)
ELIC with Propylamine in spNW15 nanodiscs with 2:1:1 POPC:POPE:POPG
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8twz
Q-score: 0.53
Dalal V, Arcario MJ, Petroff 2nd JT, Tan BK, Dietzen NM, Rau MJ, Fitzpatrick JAJ, Brannigan G, Cheng WWL
Nat Commun (2024) 15 pp. 25-25 [ DOI: doi:10.1038/s41467-023-44366-w Pubmed: 38167383 ]
ELIC5 with cysteamine in 2:1:1 POPC:POPE:POPG nanodisc in open conformation
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8vuw
Q-score: 0.538
Petroff JT, Dietzen NM, Santiago-McRae E, Deng B, Washington MS, Chen LJ, Trent Moreland K, Deng Z, Rau M, Fitzpatrick JAJ, Yuan P, Joseph TT, Henin J, Brannigan G, Cheng WWL
Nat Commun (2022) 13 pp. 7017- [ Pubmed: 36385237 DOI: doi:10.1038/s41467-022-34813-5 ]
- 2-amino-ethanethiol (77 Da, Ligand)
- Erwinia chrysanthemi ligand-gated ion channel (37 kDa, Protein from Dickeya chrysanthemi)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Elic5 (p254g/c300s/v261y/g319f/i320f) with cysteamine in 2:1:1 popc:pope:popg nanodisc (Complex from Dickeya chrysanthemi)
ELIC with Propylamine in SMA nanodiscs with 2:1:1 POPC:POPE:POPG
Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 8f32
Q-score: 0.45
Dalal V, Arcario MJ, Petroff 2nd JT, Tan BK, Dietzen NM, Rau MJ, Fitzpatrick JAJ, Brannigan G, Cheng WWL
Nat Commun (2024) 15 pp. 25-25 [ DOI: doi:10.1038/s41467-023-44366-w Pubmed: 38167383 ]
ELIC with Propylamine in saposin nanodiscs with 2:1:1 POPC:POPE:POPG
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8f33
Q-score: 0.496
Dalal V, Arcario MJ, Petroff 2nd JT, Tan BK, Dietzen NM, Rau MJ, Fitzpatrick JAJ, Brannigan G, Cheng WWL
Nat Commun (2024) 15 pp. 25-25 [ DOI: doi:10.1038/s41467-023-44366-w Pubmed: 38167383 ]
ELIC with Propylamine in spMSP1D1 nanodiscs with 2:1:1 POPC:POPE:POPG
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8f34
Q-score: 0.533
Dalal V, Arcario MJ, Petroff 2nd JT, Tan BK, Dietzen NM, Rau MJ, Fitzpatrick JAJ, Brannigan G, Cheng WWL
Nat Commun (2024) 15 pp. 25-25 [ DOI: doi:10.1038/s41467-023-44366-w Pubmed: 38167383 ]