Cryo-EM structure of human dopamine transporter in complex with dopamine
Li Y, Wang X, Meng Y, Hu T, Zhao J, Li R, Bai Q, Yuan P, Han J, Hao K, Wei Y, Qiu Y, Li N, Zhao Y
Nature (2024) 632 pp. 686-694 [ DOI: doi:10.1038/s41586-024-07796-0 Pubmed: 39112701 ]
- Chloride ion (35 Da, Ligand)
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Human dopamine transporter in complex with dopamine (Complex from Homo sapiens)
- Sodium-dependent dopamine transporter (61 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Cryo-EM structure of dopamine receptor 1 and mini-Gs complex with dopamine bound
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f0t
Q-score: 0.498
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ DOI: doi:10.1126/sciadv.abo4158 Pubmed: 35687690 ]
- Cryo-em structure of dopamine receptor 1 and mini-gs complex with dopamine bound (Complex from Homo sapiens)
- Nanobody35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Cryo-EM structure of the GDP-bound dopamine receptor 1 and mini-Gs complex with Nb35
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 7f1o
Q-score: 0.506
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ DOI: doi:10.1126/sciadv.abo4158 Pubmed: 35687690 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Cryo-em structure of the gdp-bound dopamine receptor 1 and mini-gs complex with nb35 (Complex from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GDP-bound dopamine receptor 1 and mini-Gs complex without Nb35
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7f1z
Q-score: 0.46
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ DOI: doi:10.1126/sciadv.abo4158 Pubmed: 35687690 ]
- L-dopamine (153 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Cryo-em structure of the gdp-bound dopamine receptor 1 and mini-gs complex without nb35 (Complex from Homo sapiens)
Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex with Nb35
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7f23
Q-score: 0.456
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ DOI: doi:10.1126/sciadv.abo4158 Pubmed: 35687690 ]
- Cryo-em structure of the gtp-bound dopamine receptor 1 and mini-gs complex with nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex without Nb35
Resolution: 4.16 Å
EM Method: Single-particle
Fitted PDBs: 7f24
Q-score: 0.364
Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S
Sci Adv (2022) 8 pp. eabo4158-eabo4158 [ DOI: doi:10.1126/sciadv.abo4158 Pubmed: 35687690 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- D(1a) dopamine receptor (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- L-dopamine (153 Da, Ligand)
- Cryo-em structure of the gtp-bound dopamine receptor 1 and mini-gs complex without nb35 (Complex from Homo sapiens)