SpRY-Cas9:gRNA complex targeting TGG PAM DNA
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 8spq
Q-score: 0.431
Hibshman GN, Bravo JPK, Hooper MM, Dangerfield TL, Zhang H, Finkelstein IJ, Johnson KA, Taylor DW
Nat Commun (2024) 15 pp. 3663-3663 [ DOI: doi:10.1038/s41467-024-47830-3 Pubmed: 38688943 ]
- Ts (5 kDa, DNA from Escherichia phage Lambda)
- Ts (16 kDa, DNA from Escherichia phage Lambda)
- Magnesium ion (24 Da, Ligand)
- Ternary complex of spry-cas9 with lambda 1 grna and lambda 1 dna (211 kDa, Complex from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9/csn1 (159 kDa, Protein from Streptococcus pyogenes serotype M1)
- Grna (31 kDa, RNA from Streptococcus pyogenes)
- Nts (5 kDa, DNA from Escherichia phage Lambda)
SpRY-Cas9:gRNA complex targeting TTC PAM DNA
Resolution: 3.69 Å
EM Method: Single-particle
Fitted PDBs: 8sqh
Q-score: 0.379
Hibshman GN, Bravo JPK, Hooper MM, Dangerfield TL, Zhang H, Finkelstein IJ, Johnson KA, Taylor DW
Nat Commun (2024) 15 pp. 3663-3663 [ DOI: doi:10.1038/s41467-024-47830-3 Pubmed: 38688943 ]
- Ts (5 kDa, DNA from Escherichia phage Lambda)
- Magnesium ion (24 Da, Ligand)
- Nts (16 kDa, DNA from Escherichia phage Lambda)
- Ts (4 kDa, DNA from Escherichia phage Lambda)
- Crispr-associated endonuclease cas9/csn1 (159 kDa, Protein from Streptococcus pyogenes)
- Grna (31 kDa, RNA from Streptococcus pyogenes)
- Ternary complex of spry-cas9 with lambda 1 grna and lambda 1 dna oligo (211 kDa, Complex from Streptococcus pyogenes)
SpRY-Cas9:gRNA complex targeting TAC PAM DNA
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8srs
Q-score: 0.521
Hibshman GN, Bravo JPK, Hooper MM, Dangerfield TL, Zhang H, Finkelstein IJ, Johnson KA, Taylor DW
Nat Commun (2024) 15 pp. 3663-3663 [ DOI: doi:10.1038/s41467-024-47830-3 Pubmed: 38688943 ]
- Ts (5 kDa, DNA from Escherichia phage Lambda)
- Magnesium ion (24 Da, Ligand)
- Nts (16 kDa, DNA from Escherichia phage Lambda)
- Water (18 Da, Ligand)
- Ternary complex of spry-cas9 with lambda 1 grna and lambda 1 dna (211 kDa, Complex from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9/csn1 (159 kDa, Protein from Streptococcus pyogenes)
- Grna (31 kDa, RNA from Streptococcus pyogenes)
- Ts (3 kDa, DNA from Escherichia phage Lambda)
Cryo-EM Structure of PG9RSH DU011 Fab in complex with BG505 DS-SOSIP.664
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8fk5
Q-score: 0.456
Holt GT, Gorman J, Wang S, Lowegard AU, Zhang B, Liu T, Lin BC, Louder MK, Frenkel MS, McKee K, O'Dell S, Rawi R, Shen CH, Doria-Rose NA, Kwong PD, Donald BR
Cell Rep (2023) 42 pp. 112711-112711 [ Pubmed: 37436900 DOI: doi:10.1016/j.celrep.2023.112711 ]
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Envelope glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Immunoglobulin lambda-1 light chain-like (22 kDa, Protein from Homo sapiens)
- Immunoblobulin g1 fab heavy chain variable region (fragment) (27 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cryo-em structure of pg9rsh du011 fab in complex with bg505 ds-sosip.664 (Complex from Human immunodeficiency virus 1)
Assembly intermediates of orthoreovirus captured in the cell
Resolution: 6.6 Å
EM Method: Subtomogram averaging
Fitted PDBs: 6xf7
Q-score: 0.15
Sutton G, Sun D, Fu X, Kotecha A, Hecksel CW, Clare DK, Zhang P, Stuart DI, Boyce M
Nat Commun (2020) 11 pp. 4445-4445 [ DOI: doi:10.1038/s41467-020-18243-9 Pubmed: 32895380 ]
- Reovirus slp (Complex from Mammalian orthoreovirus 3 Dearing)
- Lambda 1 protein (119 kDa, Protein from Mammalian orthoreovirus)
Assembly intermediates of orthoreovirus captured in the cell
Resolution: 6.5 Å
EM Method: Subtomogram averaging
Fitted PDBs: 6xf8
Q-score: 0.18
Sutton G, Sun D, Fu X, Kotecha A, Hecksel CW, Clare DK, Zhang P, Stuart DI, Boyce M
Nat Commun (2020) 11 pp. 4445-4445 [ DOI: doi:10.1038/s41467-020-18243-9 Pubmed: 32895380 ]
- Outer capsid protein sigma-3 (41 kDa, Protein from Reovirus type 1 (strain Lang))
- Outer capsid protein mu-1 (68 kDa, Protein from Reovirus type 1 (strain Lang))
- Reovirus slp (Complex from Mammalian orthoreovirus 3 Dearing)
- Inner capsid protein sigma-2 (47 kDa, Protein from Reovirus type 1 (strain Lang))
- Inner capsid protein lambda-1 (119 kDa, Protein from Reovirus type 1 (strain Lang))
- Mrna (guanine-n(7)-)-methyltransferase (143 kDa, Protein from Reovirus type 1 (strain Lang))