Cryo-EM structure of the Photosystem I - LHCI supercomplex from Coelastrella sp.
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8cmo
Q-score: 0.537
Fadeeva M, Klaiman D, Nelson N
To Be Published
- Light-harvesting protein of photosystem i lhca5 (24 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca2 partial (7 kDa, Protein from Coelastrella)
- Coelastrella sp. photosystem i (900 kDa, Complex from Coelastrella)
- Phylloquinone (450 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Phosphatidylinositol (887 Da, Ligand)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Photosystem i reaction center subunit iv psae (6 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca6 (24 kDa, Protein from Coelastrella)
- Beta-carotene (536 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Photosystem i subunit ix psaj (4 kDa, Protein from Coelastrella)
- Photosystem i reaction center subunit x psak (8 kDa, Protein from Coelastrella)
- Chlorophyll b (907 Da, Ligand)
- Photosystem i reaction center subunit v psag (9 kDa, Protein from Coelastrella)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Lauric acid (200 Da, Ligand)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Light-harvesting protein of photosystem i lhca8 (22 kDa, Protein from Coelastrella)
- Photosystem i reaction center subunit ii psad (16 kDa, Protein from Coelastrella)
- Water (18 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 psab (81 kDa, Protein from Coelastrella)
- Photosystem i reaction center subunit iii psaf (17 kDa, Protein from Coelastrella)
- Photosystem i p700 chlorophyll a apoprotein a1 psaa (82 kDa, Protein from Coelastrella)
- Chlorophyll a isomer (893 Da, Ligand)
- Sphingosine (299 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Light-harvesting protein of photosystem i lhca4 (22 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca9 (20 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca7 (24 kDa, Protein from Coelastrella)
- Photosystem i reaction centre subunit xi psal (12 kDa, Protein from Coelastrella)
- Octanoic acid (caprylic acid) (144 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Light-harvesting protein of photosystem i lhca3 (23 kDa, Protein from Coelastrella)
- Photosystem i subunit vii psac (8 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca1 (20 kDa, Protein from Coelastrella)
- (1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Diacyl glycerol (625 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Photosystem i reaction center subunit viii psai (4 kDa, Protein from Coelastrella)
Structure of low-light grown Chlorella ohadii Photosystem I
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6zzx
Q-score: 0.621
Caspy I, Neumann E, Fadeeva M, Liveanu V, Savitsky A, Frank A, Kalisman YL, Shkolnisky Y, Murik O, Treves H, Hartmann V, Nowaczyk MM, Schuhmann W, Rogner M, Willner I, Kaplan A, Schuster G, Nelson N, Lubitz W, Nechushtai R
Nat Plants (2021) 7 pp. 1314-1322 [ DOI: doi:10.1038/s41477-021-00983-1 Pubmed: 34462576 ]
- Phylloquinone (450 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (10 kDa, Protein from Chlorella ohadii)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit chloroplastic (8 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit chloroplastic (15 kDa, Protein from Chlorella ohadii)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- Beta,beta-caroten-4-one (550 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- Glutathione reductase (26 kDa, Protein from Chlorella ohadii)
- Benzene-1,3,5-triol (126 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Chlorella ohadii)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Chlorophyll b (907 Da, Ligand)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- Psi-f (18 kDa, Protein from Chlorella ohadii)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Water (18 Da, Ligand)
- Photosystem i (1 MDa, Complex from Chlorella ohadii)
- Photosystem i p700 chlorophyll a apoprotein a1 (82 kDa, Protein from Chlorella ohadii)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chlorella ohadii)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- Palmitic acid (256 Da, Ligand)
- Lhca7 (24 kDa, Protein from Chlorella ohadii)
- (3~{e},5~{e},7~{e})-6-methyl-8-[(6~{r})-2,2,6-trimethylcyclohexyl]octa-3,5,7-trien-2-one (260 Da, Ligand)
- Chlorophyll a isomer (893 Da, Ligand)
- Sphingosine (299 Da, Ligand)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Tripalmitoylglycerol (807 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Oleic acid (282 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Photosystem i subunit o (9 kDa, Protein from Chlorella ohadii)
- [2-((1-oxododecanoxy-(2-hydroxy-3-propanyl))-phosphonate-oxy)-ethyl]-trimethylammonium (440 Da, Ligand)
- Photosystem i reaction center subunit iv (7 kDa, Protein from Chlorella ohadii)
- Astaxanthin (596 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Diacyl glycerol (625 Da, Ligand)
- Lhca4 (23 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit xi (16 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit vi-chloroplastic-like (10 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Chlorella ohadii)
Photosystem I of Chlamydomonas reinhardtii
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 6ijj
Q-score: 0.537
Su X, Ma J, Pan X, Zhao X, Chang W, Liu Z, Zhang X, Li M
Nat Plants (2019) 5 pp. 273-281 [ DOI: doi:10.1038/s41477-019-0380-5 Pubmed: 30850819 ]
- Phylloquinone (450 Da, Ligand)
- Lhca3 (32 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca5 (30 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Lhca7 (26 kDa, Protein from Chlamydomonas reinhardtii)
- Psab (82 kDa, Protein from Chlamydomonas reinhardtii)
- Beta-carotene (536 Da, Ligand)
- Lhca1 (26 kDa, Protein from Chlamydomonas reinhardtii)
- Chlamydomonas reinhardtii psi with eight light harvesting antennae (Complex from Chlamydomonas reinhardtii)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Psak (16 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psaa (83 kDa, Protein from Chlamydomonas reinhardtii)
- Psac (8 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca4 (31 kDa, Protein from Chlamydomonas reinhardtii)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Psae (16 kDa, Protein from Chlamydomonas reinhardtii)
- Psaj (4 kDa, Protein from Chlamydomonas reinhardtii)
- Psaf (24 kDa, Protein from Chlamydomonas reinhardtii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Psai (10 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca8 (29 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca6 (35 kDa, Protein from Chlamydomonas reinhardtii)
- Dodecyl-alpha-d-maltoside (510 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Psad (21 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a (893 Da, Ligand)
- Psal (27 kDa, Protein from Chlamydomonas reinhardtii)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6ijo
Q-score: 0.469
Su X, Ma J, Pan X, Zhao X, Chang W, Liu Z, Zhang X, Li M
Nat Plants (2019) 5 pp. 273-281 [ DOI: doi:10.1038/s41477-019-0380-5 Pubmed: 30850819 ]
- Lhca9 (22 kDa, Protein from Chlamydomonas reinhardtii)
- Chainx (1 kDa, Protein from Chlamydomonas reinhardtii)
- Phylloquinone (450 Da, Ligand)
- Lhca3 (32 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca5 (30 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Lhca7 (26 kDa, Protein from Chlamydomonas reinhardtii)
- Psag (16 kDa, Protein from Chlamydomonas reinhardtii)
- Chlamydomonas reinhardtii psi with ten light harvesting antennae (Complex from Chlamydomonas reinhardtii)
- Psab (82 kDa, Protein from Chlamydomonas reinhardtii)
- Beta-carotene (536 Da, Ligand)
- Lhca1 (26 kDa, Protein from Chlamydomonas reinhardtii)
- Psah (17 kDa, Protein from Chlamydomonas reinhardtii)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Psak (16 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psaa (83 kDa, Protein from Chlamydomonas reinhardtii)
- Psac (8 kDa, Protein from Chlamydomonas reinhardtii)
- Psad (27 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca4 (31 kDa, Protein from Chlamydomonas reinhardtii)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Psae (16 kDa, Protein from Chlamydomonas reinhardtii)
- Psaj (4 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca2 (24 kDa, Protein from Chlamydomonas reinhardtii)
- Psaf (24 kDa, Protein from Chlamydomonas reinhardtii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Psai (10 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca8 (29 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca6 (35 kDa, Protein from Chlamydomonas reinhardtii)
- Dodecyl-alpha-d-maltoside (510 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Psal (27 kDa, Protein from Chlamydomonas reinhardtii)