Cryo-EM structure of the Photosystem I - LHCI supercomplex from Coelastrella sp.
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8cmo
Q-score: 0.537
Fadeeva M, Klaiman D, Nelson N
To Be Published
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Light-harvesting protein of photosystem i lhca2 partial (7 kDa, Protein from Coelastrella)
- Lauric acid (200 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 psab (81 kDa, Protein from Coelastrella)
- Photosystem i reaction center subunit viii psai (4 kDa, Protein from Coelastrella)
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit x psak (8 kDa, Protein from Coelastrella)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem i reaction centre subunit xi psal (12 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca4 (22 kDa, Protein from Coelastrella)
- Photosystem i reaction center subunit iii psaf (17 kDa, Protein from Coelastrella)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Coelastrella sp. photosystem i (900 kDa, Complex from Coelastrella)
- Light-harvesting protein of photosystem i lhca5 (24 kDa, Protein from Coelastrella)
- Chlorophyll b (907 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 psaa (82 kDa, Protein from Coelastrella)
- Photosystem i subunit ix psaj (4 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca1 (20 kDa, Protein from Coelastrella)
- Calcium ion (40 Da, Ligand)
- Photosystem i reaction center subunit v psag (9 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca9 (20 kDa, Protein from Coelastrella)
- Phosphatidylinositol (887 Da, Ligand)
- Light-harvesting protein of photosystem i lhca8 (22 kDa, Protein from Coelastrella)
- Phylloquinone (450 Da, Ligand)
- Light-harvesting protein of photosystem i lhca7 (24 kDa, Protein from Coelastrella)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Octanoic acid (caprylic acid) (144 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Light-harvesting protein of photosystem i lhca6 (24 kDa, Protein from Coelastrella)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Water (18 Da, Ligand)
- Photosystem i reaction center subunit ii psad (16 kDa, Protein from Coelastrella)
- Diacyl glycerol (625 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i reaction center subunit iv psae (6 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca3 (23 kDa, Protein from Coelastrella)
- Sphingosine (299 Da, Ligand)
- Chlorophyll a isomer (893 Da, Ligand)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Photosystem i subunit vii psac (8 kDa, Protein from Coelastrella)
Structure of low-light grown Chlorella ohadii Photosystem I
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6zzx
Q-score: 0.621
Caspy I, Neumann E, Fadeeva M, Liveanu V, Savitsky A, Frank A, Kalisman YL, Shkolnisky Y, Murik O, Treves H, Hartmann V, Nowaczyk MM, Schuhmann W, Rogner M, Willner I, Kaplan A, Schuster G, Nelson N, Lubitz W, Nechushtai R
Nat Plants (2021) 7 pp. 1314-1322 [ Pubmed: 34462576 DOI: doi:10.1038/s41477-021-00983-1 ]
- Lhca7 (24 kDa, Protein from Chlorella ohadii)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- [2-((1-oxododecanoxy-(2-hydroxy-3-propanyl))-phosphonate-oxy)-ethyl]-trimethylammonium (440 Da, Ligand)
- Photosystem i subunit o (9 kDa, Protein from Chlorella ohadii)
- Beta-carotene (536 Da, Ligand)
- Photosystem i (1 MDa, Complex from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- Chlorophyll b (907 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (8 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Chlorella ohadii)
- Palmitic acid (256 Da, Ligand)
- Lhca4 (23 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Phylloquinone (450 Da, Ligand)
- (3~{e},5~{e},7~{e})-6-methyl-8-[(6~{r})-2,2,6-trimethylcyclohexyl]octa-3,5,7-trien-2-one (260 Da, Ligand)
- Psi-f (18 kDa, Protein from Chlorella ohadii)
- Oleic acid (282 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (15 kDa, Protein from Chlorella ohadii)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chlorella ohadii)
- Chlorophyll a (893 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit xi (16 kDa, Protein from Chlorella ohadii)
- Water (18 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chlorella ohadii)
- Astaxanthin (596 Da, Ligand)
- Photosystem i reaction center subunit iv (7 kDa, Protein from Chlorella ohadii)
- Diacyl glycerol (625 Da, Ligand)
- Tripalmitoylglycerol (807 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i reaction center subunit vi-chloroplastic-like (10 kDa, Protein from Chlorella ohadii)
- Benzene-1,3,5-triol (126 Da, Ligand)
- Sphingosine (299 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (82 kDa, Protein from Chlorella ohadii)
- Glutathione reductase (26 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (10 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Chlorella ohadii)
- Beta,beta-caroten-4-one (550 Da, Ligand)
Photosystem I of Chlamydomonas reinhardtii
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 6ijj
Q-score: 0.537
Su X, Ma J, Pan X, Zhao X, Chang W, Liu Z, Zhang X, Li M
Nat Plants (2019) 5 pp. 273-281 [ DOI: doi:10.1038/s41477-019-0380-5 Pubmed: 30850819 ]
- Psac (8 kDa, Protein from Chlamydomonas reinhardtii)
- Psak (16 kDa, Protein from Chlamydomonas reinhardtii)
- Beta-carotene (536 Da, Ligand)
- Lhca3 (32 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Chlamydomonas reinhardtii psi with eight light harvesting antennae (Complex from Chlamydomonas reinhardtii)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psal (27 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca1 (26 kDa, Protein from Chlamydomonas reinhardtii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Psaj (4 kDa, Protein from Chlamydomonas reinhardtii)
- Dodecyl-alpha-d-maltoside (510 Da, Ligand)
- Psaf (24 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca7 (26 kDa, Protein from Chlamydomonas reinhardtii)
- Psad (21 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca5 (30 kDa, Protein from Chlamydomonas reinhardtii)
- Psaa (83 kDa, Protein from Chlamydomonas reinhardtii)
- Phylloquinone (450 Da, Ligand)
- Lhca6 (35 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca4 (31 kDa, Protein from Chlamydomonas reinhardtii)
- Psae (16 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a (893 Da, Ligand)
- Psab (82 kDa, Protein from Chlamydomonas reinhardtii)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Lhca8 (29 kDa, Protein from Chlamydomonas reinhardtii)
- Iron/sulfur cluster (351 Da, Ligand)
- Psai (10 kDa, Protein from Chlamydomonas reinhardtii)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6ijo
Q-score: 0.469
Su X, Ma J, Pan X, Zhao X, Chang W, Liu Z, Zhang X, Li M
Nat Plants (2019) 5 pp. 273-281 [ DOI: doi:10.1038/s41477-019-0380-5 Pubmed: 30850819 ]
- Psac (8 kDa, Protein from Chlamydomonas reinhardtii)
- Chainx (1 kDa, Protein from Chlamydomonas reinhardtii)
- Psak (16 kDa, Protein from Chlamydomonas reinhardtii)
- Beta-carotene (536 Da, Ligand)
- Lhca3 (32 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psal (27 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca1 (26 kDa, Protein from Chlamydomonas reinhardtii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Psaj (4 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca9 (22 kDa, Protein from Chlamydomonas reinhardtii)
- Chlamydomonas reinhardtii psi with ten light harvesting antennae (Complex from Chlamydomonas reinhardtii)
- Dodecyl-alpha-d-maltoside (510 Da, Ligand)
- Psaf (24 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca2 (24 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca7 (26 kDa, Protein from Chlamydomonas reinhardtii)
- Psah (17 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca5 (30 kDa, Protein from Chlamydomonas reinhardtii)
- Psaa (83 kDa, Protein from Chlamydomonas reinhardtii)
- Psag (16 kDa, Protein from Chlamydomonas reinhardtii)
- Phylloquinone (450 Da, Ligand)
- Lhca6 (35 kDa, Protein from Chlamydomonas reinhardtii)
- Lhca4 (31 kDa, Protein from Chlamydomonas reinhardtii)
- Psae (16 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a (893 Da, Ligand)
- Psab (82 kDa, Protein from Chlamydomonas reinhardtii)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Lhca8 (29 kDa, Protein from Chlamydomonas reinhardtii)
- Iron/sulfur cluster (351 Da, Ligand)
- Psai (10 kDa, Protein from Chlamydomonas reinhardtii)
- Psad (27 kDa, Protein from Chlamydomonas reinhardtii)