Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 440-2 and 454-3
Silmon de Monerri NC, Che Y, Lees JA, Jasti J, Wu H, Griffor MC, Kodali S, Hawkins JC, Lypowy J, Ponce C, Curley K, Esadze A, Carcamo J, McLellan T, Keeney D, Illenberger A, Matsuka YV, Shanker S, Chorro L, Gribenko AV, Han S, Anderson AS, Donald RGK
PLoS Pathog (2025) 21 pp. e1012325-e1012325 [ Pubmed: 39970181 DOI: doi:10.1371/journal.ppat.1012325 ]
- 440-2 fab light chain (24 kDa, Protein from Mus musculus)
- 440-2 fab heavy chain (24 kDa, Protein from Mus musculus)
- 445-3 fab heavy chain (24 kDa, Protein from Mus musculus)
- 445-3 fab light chain (23 kDa, Protein from Mus musculus)
- Type 1 fimbrin d-mannose specific adhesin fimh, donor strand complemented with fimg peptide 'triple mutant' (32 kDa, Protein from Escherichia coli)
- Fimh-dsg tm in complex with fabs 440-2 and 445-3 (Complex)
- Fimh-dsg tm (Complex from Escherichia coli)
- Fab 445-3 (Complex from Mus musculus)
- Fab 440-2 (Complex from Mus musculus)
The Anti-Mullerian Hormone prodomain in complex with the growth factor and 6E11 Fab in C1 symmetry
Howard JA, Hok L, Cate RL, Sanford NJ, Hart KN, Leach EAE, Bruening AS, Nagykery N, Donahoe PK, Pepin D, Thompson TB
PNAS (2025) 122 pp. e2418088122-e2418088122 [ Pubmed: 39805014 DOI: doi:10.1073/pnas.2418088122 ]
- Muellerian-inhibiting factor (11 kDa, Protein from Homo sapiens)
- 6e11 antibody kappa light chain (23 kDa, Protein from Mus musculus)
- Fab-bound amh procomplex (240 kDa, Complex from Homo sapiens)
- 6e11 antibody igg2a heavy chain (24 kDa, Protein from Mus musculus)
- Muellerian-inhibiting factor (45 kDa, Protein from Homo sapiens)
The Anti-Mullerian Hormone prodomain in complex with the growth factor and 6E11 Fab in C2 symmetry
Howard JA, Hok L, Cate RL, Sanford NJ, Hart KN, Leach EAE, Bruening AS, Nagykery N, Donahoe PK, Pepin D, Thompson TB
PNAS (2025) 122 pp. e2418088122-e2418088122 [ Pubmed: 39805014 DOI: doi:10.1073/pnas.2418088122 ]
- Muellerian-inhibiting factor (11 kDa, Protein from Homo sapiens)
- 6e11 antibody kappa light chain (23 kDa, Protein from Mus musculus)
- Fab-bound amh procomplex (240 kDa, Complex from Homo sapiens)
- 6e11 antibody igg2a heavy chain (24 kDa, Protein from Mus musculus)
- Muellerian-inhibiting factor (45 kDa, Protein from Homo sapiens)
Influenza A virus Hemagglutinin H5/Vietnam/1204/2004 in complex with D04 Fab
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8ume
Q-score: 0.462
Feldman J, Ramos ASF, Vu M, Maurer DP, Rosado VC, Lingwood D, Bajic G, Schmidt AG
Sci Immunol (2025) 10 pp. eado9572-eado9572 [ Pubmed: 39854479 DOI: doi:10.1126/sciimmunol.ado9572 ]
- Hemagglutinin (62 kDa, Protein from Influenza A virus)
- Fab heavy chain (24 kDa, Protein from Homo sapiens)
- Ha/fab (350 MDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fab light chain (24 kDa, Protein from Homo sapiens)
Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 329-2 and 454-3
Silmon de Monerri NC, Che Y, Lees JA, Jasti J, Wu H, Griffor MC, Kodali S, Hawkins JC, Lypowy J, Ponce C, Curley K, Esadze A, Carcamo J, McLellan T, Keeney D, Illenberger A, Matsuka YV, Shanker S, Chorro L, Gribenko AV, Han S, Anderson AS, Donald RGK
PLoS Pathog (2025) 21 pp. e1012325-e1012325 [ Pubmed: 39970181 DOI: doi:10.1371/journal.ppat.1012325 ]
- Fab 329-2 light chain (23 kDa, Protein from Mus musculus)
- Ternary complex of e. coli fimh with fabs 329-2 and 454-3 (Complex from Escherichia coli)
- Type 1 fimbrin d-mannose specific adhesin fimh, donor strand complemented with fimg peptide 'triple mutant' (32 kDa, Protein from Escherichia coli)
- Fab 329-2 heavy chain (24 kDa, Protein from Mus musculus)
- Fab 454-3 light chain (23 kDa, Protein from Mus musculus)
- Fab 454-3 heavy chain (23 kDa, Protein from Mus musculus)
GP38-GnH-DS-Gc in the pre-fusion conformation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9b8j
Q-score: 0.474
McFadden E, Monticelli SR, Wang A, Ramamohan AR, Batchelor TG, Kuehne AI, Bakken RR, Tse AL, Chandran K, Herbert AS, McLellan JS
Cell (2025) 188 pp. 303-315.e13 [ Pubmed: 39701101 DOI: doi:10.1016/j.cell.2024.11.008 ]
- Adi-46152 heavy chain (52 kDa, Protein from Homo sapiens)
- Adi-36125 heavy chain (51 kDa, Protein from Homo sapiens)
- Gp38-gnh-ds-gc in complex with adi-46152 and adi-36125 fabs (Complex from Orthonairovirus haemorrhagiae)
- Adi-36125 light chain (26 kDa, Protein from Homo sapiens)
- Glycoprotein n (10 kDa, Protein from Crimean-Congo hemorrhagic fever virus strain IbAr10200)
- Gp38 (30 kDa, Protein from Crimean-Congo hemorrhagic fever virus strain IbAr10200)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein c (59 kDa, Protein from Crimean-Congo hemorrhagic fever virus strain IbAr10200)
- Adi-46152 light chain (25 kDa, Protein from Homo sapiens)
Influenza A virus Hemagglutinin H3/Darwin/6/2021 in complex with Fab ADI-85666
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 9bdf
Q-score: 0.563
Maurer D, Ferreira Ramos AS, Bajic G, Aaron GS
To Be Published
- Adi-85666 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Hemagglutinin (57 kDa, Protein from Influenza A virus)
- Adi-85666 fab light chain (23 kDa, Protein from Homo sapiens)
- Ha/fab (350 MDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of VRC44.01 Fab in complex with 3BNC117-purified C1080.c3 RnS SOSIP.664 HIV-1 Env trimer
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 9bio
Q-score: 0.547
Cale EM, Shen CH, Olia AS, Radakovich NA, Rawi R, Yang Y, Ambrozak DR, Bennici AK, Chuang GY, Crooks ED, Driscoll JI, Lin BC, Louder MK, Madden PJ, Messina MA, Osawa K, Stewart-Jones GBE, Verardi R, Vrakas Z, Xie D, Zhang B, Binley JM, Connors M, Koup RA, Pierson TC, Doria-Rose NA, Kwong PD, Mascola JR, Gorman J
Cell Rep (2024) 43 pp. 115010-115010 [ Pubmed: 39675002 DOI: doi:10.1016/j.celrep.2024.115010 ]
- 3bnc117 heavy chain (24 kDa, Protein from Homo sapiens)
- Vrc44 and 3bnc117 fabs with repaired c1080 env trimer (Complex from Human immunodeficiency virus 1)
- Phosphate ion (94 Da, Ligand)
- Envelope glycoprotein gp160 (54 kDa, Protein from Human immunodeficiency virus 1)
- Vrc44.01 light chain (11 kDa, Protein from Homo sapiens)
- 3bnc117 light chain (23 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (19 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Vrc44.01 heavy chain (13 kDa, Protein from Homo sapiens)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8vym
Q-score: 0.476
Sponholtz MR, Byrne PO, Lee AG, Ramamohan AR, Goldsmith JA, McCool RS, Zhou L, Johnson NV, Hsieh CL, Connors M, Karthigeyan KP, Crooks CM, Fuller AS, Campbell JD, Permar SR, Maynard JA, Yu D, Bottomley MJ, McLellan JS
PNAS (2024) 121 pp. e2404250121-e2404250121 [ DOI: doi:10.1073/pnas.2404250121 Pubmed: 39231203 ]
- 7h3 fab light chain (22 kDa, Protein from Homo sapiens)
- 1g2 fab heavy chain (24 kDa, Protein from Homo sapiens)
- Envelope glycoprotein b (88 kDa, Protein from Human betaherpesvirus 5)
- Soluble ectodomain of human cytomegalovirus (hcmv) glycoprotein b (gb) in the postfusion conformation in complex with 1g2 and 7h3 fabs (Complex)
- 1g2 fab light chain (22 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 7h3 fab heavy chain (25 kDa, Protein from Homo sapiens)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8vyn
Q-score: 0.551
Sponholtz MR, Byrne PO, Lee AG, Ramamohan AR, Goldsmith JA, McCool RS, Zhou L, Johnson NV, Hsieh CL, Connors M, Karthigeyan KP, Crooks CM, Fuller AS, Campbell JD, Permar SR, Maynard JA, Yu D, Bottomley MJ, McLellan JS
PNAS (2024) 121 pp. e2404250121-e2404250121 [ DOI: doi:10.1073/pnas.2404250121 Pubmed: 39231203 ]
- Soluble ectodomain of human cytomegalovirus (hcmv) glycoprotein b (gb) stabilized in a prefusion-like conformation in complex with 1g2 and 7h3 (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 7h3 fab light chain (22 kDa, Protein from Homo sapiens)
- 1g2 fab heavy chain (24 kDa, Protein from Homo sapiens)
- 1g2 fab light chain (22 kDa, Protein from Homo sapiens)
- Envelope glycoprotein b (88 kDa, Protein from Human betaherpesvirus 5)
- 7h3 fab heavy chain (25 kDa, Protein from Homo sapiens)