Cryo-EM structure of light harvesting complex 2 from Rba. sphaeroides.
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 7pbw
Q-score: 0.76
Qian P, Swainsbury DJK, Croll TI, Castro-Hartmann P, Divitini G, Sader K, Hunter CN
Biochemistry (2021) 60 pp. 3302-3314 [ Pubmed: 34699186 DOI: doi:10.1021/acs.biochem.1c00576 ]
- Water (18 Da, Ligand)
- Light-harvesting protein b-800/850 beta chain (4 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Light-harvesting protein b-800/850 alpha chain (5 kDa, Protein from Cereibacter sphaeroides 2.4.1)
- Lauryl dimethylamine-n-oxide (229 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- Light harvesting complex 2 (123 kDa, Complex from Cereibacter sphaeroides 2.4.1)
- Calcium ion (40 Da, Ligand)
- Spheroidene (568 Da, Ligand)
Cryo-EM structure of the Rhodobacter sphaeroides RC-LH1-PufXY monomer complex at 2.5 A
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7pil
Q-score: 0.64
Qian P, Swainsbury DJK, Croll TI, Salisbury JH, Martin EC, Jackson PJ, Hitchcock A, Castro-Hartmann P, Sader K, Hunter CN
Biochem J (2021) 478 pp. 3775-3790 [ Pubmed: 34590677 DOI: doi:10.1042/BCJ20210631 ]
- Ubiquinone-10 (863 Da, Ligand)
- Rc-y (5 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- Light harvesting complex (Complex from Cereibacter sphaeroides 2.4.1)
- Ubiquinone-1 (250 Da, Ligand)
- Water (18 Da, Ligand)
- Reaction center protein l chain (31 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- Bacteriopheophytin a (889 Da, Ligand)
- Reaction center protein h chain (26 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- Light-harvesting protein b-875 beta chain (4 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- Intrinsic membrane protein pufx (5 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
- (2r,5r,11r,14r)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-2,14-bis(tetradecanoyloxy)-4,6,10,12,16-pentaoxa-5,11-diphosphatriacont-1-yl tetradecanoate (1 kDa, Ligand)
- Reaction center protein m chain (34 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158))
- Spheroidene (568 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
- Light-harvesting protein b-875 alpha chain (6 kDa, Protein from Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.))
LH2 complex from Marichromatium purpuratum
Resolution: 2.38 Å
EM Method: Single-particle
Fitted PDBs: 6zxa
Q-score: 0.675
Gardiner AT, Naydenova K, Castro-Hartmann P, Nguyen-Phan TC, Russo CJ, Sader K, Hunter CN, Cogdell RJ, Qian P
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abe4650 Pubmed: 33579696 ]
- Light-harvesting protein b:800-850 subunit beta (5 kDa, Protein from Marichromatium purpuratum 984)
- Bacteriochlorophyll a (911 Da, Ligand)
- Lhc domain-containing protein (8 kDa, Protein from Marichromatium purpuratum 984)
- Light harvesting complex 2 (lh2) (110 kDa, Complex from Marichromatium purpuratum)
- 9-cis-okenone (578 Da, Ligand)
- All-trans okenone (578 Da, Ligand)
RC-LH1(16) complex from Rhodopseudomonas palustris
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6z5r
Q-score: 0.603
Swainsbury DJK, Qian P, Jackson PJ, Faries KM, Niedzwiedzki DM, Martin EC, Farmer DA, Malone LA, Thompson RF, Ranson NA, Canniffe DP, Dickman MJ, Holten D, Kirmaier C, Hitchcock A, Hunter CN
Sci Adv (2021) 7 [ Pubmed: 33523887 DOI: doi:10.1126/sciadv.abe2631 ]
- Reaction center-light harvesting complex 1 (3 MDa, Complex from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Ubiquinone-10 (863 Da, Ligand)
- (6~{r},10~{s},14~{r},19~{r},23~{s},24~{e},27~{s},28~{e})-2,6,10,14,19,23,27,31-octamethyldotriaconta-24,28-dien-2-ol (575 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Water (18 Da, Ligand)
- Reaction center protein m chain (34 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- Light-harvesting complex 1 alpha chain (5 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Reaction center protein l chain (30 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Bacteriopheophytin a (889 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
- Light-harvesting complex 1 beta chain (5 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- H subunit of photosynthetic reaction center complex (27 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Spirilloxanthin (596 Da, Ligand)
RC-LH1(14)-W complex from Rhodopseudomonas palustris
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 6z5s
Q-score: 0.593
Swainsbury DJK, Qian P, Jackson PJ, Faries KM, Niedzwiedzki DM, Martin EC, Farmer DA, Malone LA, Thompson RF, Ranson NA, Canniffe DP, Dickman MJ, Holten D, Kirmaier C, Hitchcock A, Hunter CN
Sci Adv (2021) 7 [ Pubmed: 33523887 DOI: doi:10.1126/sciadv.abe2631 ]
- Ubiquinone-10 (863 Da, Ligand)
- Light-harvesting complex 1 beta chain (7 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (6~{r},10~{s},14~{r},19~{r},23~{s},24~{e},27~{s},28~{e})-2,6,10,14,19,23,27,31-octamethyldotriaconta-24,28-dien-2-ol (575 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Spirilloxanthin (596 Da, Ligand)
- Reaction center protein m chain (34 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Water (18 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
- Reaction center protein l chain (30 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Light harvesting complex 1 protein w (10 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- Bacteriopheophytin a (889 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Reaction center-light harvesting complex 1-protein w (3 MDa, Complex from Rhodopseudomonas palustris CGA009)
- H subunit of photosynthetic reaction center complex (27 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Light-harvesting complex 1 alpha chain (7 kDa, Protein from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009))
Reaction centre light harvesting complex 1 from Blc. virids
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 6et5
Q-score: 0.469
Qian P, Siebert CA, Wang P, Canniffe DP, Hunter CN
Nature (2018) 556 pp. 203-208 [ Pubmed: 29618818 DOI: doi:10.1038/s41586-018-0014-5 ]
- Light-harvesting protein b-1015 alpha chain (6 kDa, Protein from Rhodopseudomonas viridis)
- 15-cis-1,2-dihydroneurosporene (540 Da, Ligand)
- Reaction center protein m chain (35 kDa, Protein from Rhodopseudomonas viridis)
- Reaction center protein l chain (30 kDa, Protein from Rhodopseudomonas viridis)
- All-trans-1,2-dihydroneurosporene (540 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Sulfate ion (96 Da, Ligand)
- Lauryl dimethylamine-n-oxide (229 Da, Ligand)
- Light-harvesting protein b-1015 beta chain (6 kDa, Protein from Rhodopseudomonas viridis)
- Light-harvesting protein b-1015 gamma chain (2 kDa, Protein from Rhodopseudomonas viridis)
- Photosynthetic reaction center cytochrome c subunit (37 kDa, Protein from Rhodopseudomonas viridis)
- Ubiquinone-9 (795 Da, Ligand)
- Bacteriopheophytin b (887 Da, Ligand)
- Menaquinone-9 (785 Da, Ligand)
- Reaction centre-light harvesting complex 1 (rc-lh1) (414 kDa, Complex from Blastochloris viridis)
- Fe (iii) ion (55 Da, Ligand)
- Bacteriochlorophyll b (909 Da, Ligand)
- Reaction center protein h chain (28 kDa, Protein from Rhodopseudomonas viridis)