Methyl-coenzyme M reductase activation complex binding to the A2 component
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8s7v
Q-score: 0.569
Ramirez-Amador F, Paul S, Kumar A, Schuller JM
To Be Published
- Magnesium ion (24 Da, Ligand)
- Factor 430 (906 Da, Ligand)
- Methyl-coenzyme m reductase subunit beta (46 kDa, Protein from Methanococcus maripaludis)
- O-phosphono-n-{(2e)-7-[(2-sulfoethyl)dithio]hept-2-enoyl}-l-threonine (481 Da, Ligand)
- Glycine betaine/carnitine/choline transport atp-binding protein opuca (59 kDa, Protein from Methanococcus maripaludis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Fefe cofactor (747 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Methanogenesis marker protein 17 (21 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase subunit gamma (29 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase subunit alpha (61 kDa, Protein from Methanococcus maripaludis)
- Methanogenesis marker protein 7 (35 kDa, Protein from Methanococcus maripaludis)
- Upf0288 protein mmarc6_0796 (56 kDa, Protein from Methanococcus maripaludis)
- Duf2098 domain-containing protein (10 kDa, Protein from Methanococcus maripaludis)
- Coenzyme b (343 Da, Ligand)
- Methyl-coenzyme m reductase operon protein c (24 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase activation complex binding to a2 component (480 kDa, Complex from Methanococcus maripaludis)
- 1-thioethanesulfonic acid (142 Da, Ligand)
Methyl-coenzyme M reductase activation complex without the A2 component
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 8s7x
Q-score: 0.559
Ramirez-Amador F, Paul S, Kumar A, Schuller JM
To Be Published
- Duf2098 domain-containing protein (10 kDa, Protein from Methanococcus maripaludis)
- Factor 430 (906 Da, Ligand)
- O-phosphono-n-{(2e)-7-[(2-sulfoethyl)dithio]hept-2-enoyl}-l-threonine (481 Da, Ligand)
- Fefe cofactor (747 Da, Ligand)
- Methanogenesis marker protein 17 (21 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase subunit gamma (29 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase subunit alpha (61 kDa, Protein from Methanococcus maripaludis)
- Methanogenesis marker protein 7 (35 kDa, Protein from Methanococcus maripaludis)
- Upf0288 protein mmarc6_0796 (56 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase activation complex without a2 component (420 kDa, Complex from Methanococcus maripaludis)
- Coenzyme b (343 Da, Ligand)
- Methyl-coenzyme m reductase operon protein c (24 kDa, Protein from Methanococcus maripaludis)
- 1-thioethanesulfonic acid (142 Da, Ligand)
- Methyl-coenzyme m reductase subunit beta (46 kDa, Protein from Methanococcus maripaludis)
Methyl-coenzyme M reductase activation complex binding to the A2 component after incubation with ATP
Resolution: 2.14 Å
EM Method: Single-particle
Fitted PDBs: 9h1l
Q-score: 0.599
Ramirez-Amador F, Paul S, Kumar A, Schuller JM
To Be Published
- Magnesium ion (24 Da, Ligand)
- Factor 430 (906 Da, Ligand)
- Methyl-coenzyme m reductase subunit beta (46 kDa, Protein from Methanococcus maripaludis)
- O-phosphono-n-{(2e)-7-[(2-sulfoethyl)dithio]hept-2-enoyl}-l-threonine (481 Da, Ligand)
- Glycine betaine/carnitine/choline transport atp-binding protein opuca (59 kDa, Protein from Methanococcus maripaludis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Fefe cofactor (747 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Methanogenesis marker protein 17 (21 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase subunit gamma (29 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase subunit alpha (61 kDa, Protein from Methanococcus maripaludis)
- Methanogenesis marker protein 7 (35 kDa, Protein from Methanococcus maripaludis)
- Upf0288 protein mmarc6_0796 (56 kDa, Protein from Methanococcus maripaludis)
- Duf2098 domain-containing protein (10 kDa, Protein from Methanococcus maripaludis)
- Coenzyme b (343 Da, Ligand)
- Methyl-coenzyme m reductase operon protein c (24 kDa, Protein from Methanococcus maripaludis)
- Methyl-coenzyme m reductase activation complex binding to the a2 component after incubation with atp (500 kDa, Complex from Methanococcus maripaludis)
- 1-thioethanesulfonic acid (142 Da, Ligand)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8gf5
Q-score: 0.502
Chadwick GL, Joiner AMN, Ramesh S, Mitchell DA, Nayak DD
PNAS (2023) 120 pp. e2302815120-e2302815120 [ DOI: doi:10.1073/pnas.2302815120 Pubmed: 37307484 ]
- Methyl-coenzyme m reductase subunit beta (45 kDa, Protein from Methanosarcina acetivorans C2A)
- Factor 430 (906 Da, Ligand)
- Methyl-coenzyme m reductase subunit alpha (62 kDa, Protein from Methanosarcina acetivorans C2A)
- Methyl coenzyme m reductase, subunit d (21 kDa, Protein from Methanosarcina acetivorans C2A)
- Assembly intermediate of the mcr complex bound to mcrd (Complex from Methanosarcina acetivorans C2A)
- Methyl-coenzyme m reductase subunit gamma (27 kDa, Protein from Methanosarcina acetivorans C2A)
- Coenzyme b (343 Da, Ligand)
- 1-thioethanesulfonic acid (142 Da, Ligand)
Apo-apo MCR assembly intermediate
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8gf6
Q-score: 0.455
Chadwick GL, Joiner AMN, Ramesh S, Mitchell DA, Nayak DD
PNAS (2023) 120 pp. e2302815120-e2302815120 [ DOI: doi:10.1073/pnas.2302815120 Pubmed: 37307484 ]
- Methyl-coenzyme m reductase subunit beta (45 kDa, Protein from Methanosarcina acetivorans C2A)
- Methyl-coenzyme m reductase subunit alpha (62 kDa, Protein from Methanosarcina acetivorans C2A)
- Methyl coenzyme m reductase, subunit d (21 kDa, Protein from Methanosarcina acetivorans C2A)
- Assembly intermediate of the mcr complex bound to mcrd (Complex from Methanosarcina acetivorans C2A)
- Methyl-coenzyme m reductase subunit gamma (27 kDa, Protein from Methanosarcina acetivorans C2A)