CryoEM structure of Polytomella F-ATP synthase, Full dimer, composite map
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6rd4
Q-score: 0.557
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mitochondrial f-atp synthase dimer from polytomella sp. pringsheim 198.80 (1 MDa, Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Magnesium ion (24 Da, Ligand)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Mitochondrial atp synthase subunit asa2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Cryo-EM structure of Polytomella F-ATP synthase, Primary rotary state 2, monomer-masked refinement
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rdd
Q-score: 0.546
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Zinc ion (65 Da, Ligand)
- Water (18 Da, Ligand)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Polytomella f-atp synthase (Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Cryo-EM structure of Polytomella F-ATP synthase, Rotary substate 1B, composite map
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6rdk
Q-score: 0.458
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mitochondrial f-atp synthase dimer from polytomella sp. pringsheim 198.80 (1 MDa, Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Magnesium ion (24 Da, Ligand)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Mitochondrial atp synthase subunit asa2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Cryo-EM structure of Polytomella F-ATP synthase, Rotary substate 1F, monomer-masked refinement
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6rdx
Q-score: 0.428
Murphy BJ, Klusch N, Langer J, Mills DJ, Yildiz O, Kuhlbrandt W
Science (2019) 364 [ Pubmed: 31221832 DOI: doi:10.1126/science.aaw9128 ]
- Mitochondrial atp synthase subunit c (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa7 (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Epsilon: polytomella f-atp synthase epsilon subunit (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mitochondrial f-atp synthase dimer from polytomella sp. pringsheim 198.80 (1 MDa, Complex from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 14 kda protein (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase associated protein asa4 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit alpha (60 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial f1f0 atp synthase associated 32 kda protein (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Atp synthase gamma chain, mitochondrial (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit 6 (34 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit delta (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Asa-10: polytomella f-atp synthase associated subunit 10 (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase associated protein asa1 (68 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa9 (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Atp synthase subunit beta (61 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Magnesium ion (24 Da, Ligand)
- Mitochondrial atp synthase subunit oscp (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- Mitochondrial atp synthase subunit asa2 (44 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa8 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mitochondrial atp synthase subunit asa6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)