Electron Microscopy Data Bank

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Yeast 26S proteasome bound to ubiquitinated substrate (5T motor state)

Release date: Oct. 17, 2018
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 6ef2
Q-score: 0.43

de la Pena AH, Goodall EA, Gates SN, Lander GC, Martin A

Science (2018) 362 [ DOI: doi:10.1126/science.aav0725 Pubmed: 30309908 ]

Sample:

Substrate-engaged 26s proteasome in the 5t state (Complex)
26s proteasome regulatory subunit 6a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Adenosine-5'-diphosphate (427 Da, Ligand)
Model substrate polypeptide (1 kDa, Protein from Homo sapiens)
Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Proteasome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Proteasome subunit alpha type-1 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Proteasome subunit alpha type-4 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
26s proteasome regulatory subunit 8 homolog (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
26s proteasome regulatory subunit 4 homolog (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
26s proteasome subunit rpt4 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Proteasome subunit alpha type-5 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
26s proteasome regulatory subunit 6b homolog (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Adenosine-5'-triphosphate (507 Da, Ligand)
Proteasome subunit alpha type-3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
26s proteasome regulatory subunit 7 homolog (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Probable proteasome subunit alpha type-7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Substrate (Complex from Homo sapiens)

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