Electron Microscopy Data Bank

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Mycobacterium smegmatis ATP synthase state 1a

Release date: Oct. 27, 2021
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 7njk
Q-score: 0.608

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Magnesium ion (24 Da, Ligand)
Atp synthase gamma chain (33 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Adenosine-5'-diphosphate (427 Da, Ligand)
Mycobacterium smegmatis atp synthase (Complex from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase epsilon chain (13 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))

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EMD-12382

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Mycobacterium smegmatis ATP synthase state 1b

Release date: Oct. 27, 2021
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 7njl
Q-score: 0.577

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Magnesium ion (24 Da, Ligand)
Atp synthase gamma chain (33 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Adenosine-5'-diphosphate (427 Da, Ligand)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase epsilon chain (13 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Mycobacterium smegmatis atp synthase (Complex from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))

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EMD-12387

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Mycobacterium smegmatis ATP synthase state 1c

Release date: Oct. 27, 2021
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 7njm
Q-score: 0.562

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Magnesium ion (24 Da, Ligand)
Atp synthase gamma chain (33 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Adenosine-5'-diphosphate (427 Da, Ligand)
Mycobacterium smegmatis atp synthase (Complex from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b (17 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase epsilon chain (13 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))

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EMD-12392

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Mycobacterium smegmatis ATP synthase state 1d

Release date: Oct. 27, 2021
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 7njn
Q-score: 0.56

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Magnesium ion (24 Da, Ligand)
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Mycobacterium smegmatis atp synthase (Complex from Mycolicibacterium smegmatis)
Adenosine-5'-diphosphate (427 Da, Ligand)
Atp synthase epsilon chain (13 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Atp synthase gamma chain (33 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)

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EMD-12397

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Mycobacterium smegmatis ATP synthase state 1e

Release date: Oct. 27, 2021
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7njo
Q-score: 0.532

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Magnesium ion (24 Da, Ligand)
Atp synthase gamma chain (33 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Adenosine-5'-diphosphate (427 Da, Ligand)
Mycobacterium smegmatis atp synthase (Complex from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase epsilon chain (13 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))

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EMD-12402

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Mycobacterium smegmatis ATP synthase state 2

Release date: Oct. 27, 2021
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 7njp
Q-score: 0.536

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Magnesium ion (24 Da, Ligand)
Atp synthase gamma chain (33 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Adenosine-5'-diphosphate (427 Da, Ligand)
Mycobacterium smegmatis atp synthase (Complex from Mycolicibacterium smegmatis MC2 155)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase epsilon chain (13 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))

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EMD-12407

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Mycobacterium smegmatis ATP synthase state 3a

Release date: Oct. 27, 2021
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 7njq
Q-score: 0.596

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Magnesium ion (24 Da, Ligand)
Atp synthase gamma chain (33 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Adenosine-5'-diphosphate (427 Da, Ligand)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase epsilon chain (13 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Mycobacterium smegmatis atp synthase (547 kDa, Complex from Mycolicibacterium smegmatis MC2 155)

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EMD-12412

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Mycobacterium smegmatis ATP synthase state 3b

Release date: Oct. 27, 2021
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 7njr
Q-score: 0.599

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Magnesium ion (24 Da, Ligand)
Atp synthase gamma chain (33 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Adenosine-5'-diphosphate (427 Da, Ligand)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase epsilon chain (13 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Mycobacterium smegmatis atp synthase (547 kDa, Complex from Mycolicibacterium smegmatis MC2 155)

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EMD-12417

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Mycobacterium smegmatis ATP synthase state 3c

Release date: Oct. 27, 2021
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 7njs
Q-score: 0.614

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Magnesium ion (24 Da, Ligand)
Atp synthase gamma chain (33 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Adenosine-5'-triphosphate (507 Da, Ligand)
Water (18 Da, Ligand)
Adenosine-5'-diphosphate (427 Da, Ligand)
Atp synthase subunit beta (51 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Mycobacterium smegmatis atp synthase (547 kDa, Complex from Mycolicibacterium smegmatis)
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase epsilon chain (13 kDa, Protein from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit alpha (58 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))

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EMD-12424

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Mycobacterium smegmatis ATP synthase Fo combined class 2

Release date: Oct. 27, 2021
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7njv
Q-score: 0.595

Montgomery MG, Petri J, Spikes TE, Walker JE

PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2111899118 Pubmed: 34782468 ]

Sample:

Atp synthase subunit c (8 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit a (27 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Bedaquiline (555 Da, Ligand)
Atp synthase subunit b-delta (47 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Atp synthase subunit b (19 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Mycobacterium smegmatis atp synthase (547 kDa, Complex from Mycolicibacterium smegmatis MC2 155)

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