Cryo-EM structure of Arabidopsis thaliana complex-I (closed conformation)
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 7ar8
Q-score: 0.464
Klusch N, Senkler J, Yildiz O, Kuehlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ DOI: doi:10.1093/plcell/koab092 Pubmed: 33768254 ]
- Nadh dehydrogenase subunit 4l (11 kDa, Protein from Mouse-ear cress)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Mouse-ear cress)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- P1 (11 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (11 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8-a, mitochondrial (25 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-a (16 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from Mouse-ear cress)
- Nadh-ubiquinone oxidoreductase chain 4 (55 kDa, Protein from Mouse-ear cress)
- B15 -- 1 beta subcomplex subunit 4 (8 kDa, Protein from Mouse-ear cress)
- 1,2-dicaproyl-sn-phosphatidyl-l-serine (455 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (13 kDa, Protein from Mouse-ear cress)
- At2g46540/f11c10.23 (6 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-b (12 kDa, Protein from Mouse-ear cress)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (36 kDa, Protein from Mouse-ear cress)
- Probable nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial (19 kDa, Protein from Mouse-ear cress)
- At3g07480.1 (17 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3 (22 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-a (8 kDa, Protein from Mouse-ear cress)
- Phosphatidylethanolamine (734 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (28 kDa, Protein from Mouse-ear cress)
- Ubiquinone-9 (795 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-b (11 kDa, Protein from Mouse-ear cress)
- Uncharacterized protein at2g27730, mitochondrial (11 kDa, Protein from Mouse-ear cress)
- Cryo-em structure of arabidopsis thaliana complex-i (closed conformation) (Complex from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 (7 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (17 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase subunit 7 (45 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (12 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (24 kDa, Protein from Mouse-ear cress)
- Excitatory amino acid transporter (9 kDa, Protein from Mouse-ear cress)
- Phosphatidylinositol (887 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (53 kDa, Protein from Mouse-ear cress)
- Esss subunit of nadh:ubiquinone oxidoreductase (complex i) protein (12 kDa, Protein from Mouse-ear cress)
- Transmembrane protein (9 kDa, Protein from Mouse-ear cress)
- Probable nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (18 kDa, Protein from Mouse-ear cress)
- Gamma carbonic anhydrase-like 2, mitochondrial (27 kDa, Protein from Mouse-ear cress)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Gamma carbonic anhydrase 1, mitochondrial (30 kDa, Protein from Mouse-ear cress)
- Gamma carbonic anhydrase 2, mitochondrial (30 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (7 kDa, Protein from Mouse-ear cress)
- Fe (iii) ion (55 Da, Ligand)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein 1, mitochondrial (13 kDa, Protein from Mouse-ear cress)
- Nadh-ubiquinone oxidoreductase chain 2 (55 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (43 kDa, Protein from Mouse-ear cress)
- Unknown (2 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5-b (9 kDa, Protein from Mouse-ear cress)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Furry (15 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (81 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (10 kDa, Protein from Mouse-ear cress)
- Acyl carrier protein 2, mitochondrial (14 kDa, Protein from Mouse-ear cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (13 kDa, Protein from Mouse-ear cress)
- Nadh-ubiquinone oxidoreductase chain 5 (74 kDa, Protein from Mouse-ear cress)
- Nadh-ubiquinone oxidoreductase chain 6 (23 kDa, Protein from Mouse-ear cress)
- At4g16450 (11 kDa, Protein from Mouse-ear cress)
Cryo-EM structure of Arabidopsis thaliana Complex-I (complete composition)
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 7arb
Q-score: 0.544
Klusch N, Senkler J, Yildiz O, Kuehlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ DOI: doi:10.1093/plcell/koab092 Pubmed: 33768254 ]
- Probable nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial (19 kDa, Protein from Arabidopsis thaliana)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (13 kDa, Protein from Arabidopsis thaliana)
- At4g16450 (11 kDa, Protein from Arabidopsis thaliana)
- Probable nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (18 kDa, Protein from Arabidopsis thaliana)
- Gamma carbonic anhydrase-like 2, mitochondrial (27 kDa, Protein from Arabidopsis thaliana)
- Furry (15 kDa, Protein from Arabidopsis thaliana)
- 1,2-dicaproyl-sn-phosphatidyl-l-serine (455 Da, Ligand)
- Nadh dehydrogenase subunit 7 (45 kDa, Protein from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 2 (55 kDa, Protein from Arabidopsis thaliana)
- Transmembrane protein (9 kDa, Protein from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 6 (23 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 (7 kDa, Protein from Arabidopsis thaliana)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (13 kDa, Protein from Arabidopsis thaliana)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-a (16 kDa, Protein from Arabidopsis thaliana)
- Gamma carbonic anhydrase 1, mitochondrial (30 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (12 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (24 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (17 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase subunit 4l (11 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-b (12 kDa, Protein from Arabidopsis thaliana)
- Excitatory amino acid transporter (9 kDa, Protein from Arabidopsis thaliana)
- Phosphatidylethanolamine (734 Da, Ligand)
- Ubiquinone-9 (795 Da, Ligand)
- Cryo-em structure of arabidopsis thaliana complex-i (complete composition) (Complex from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-a (8 kDa, Protein from Arabidopsis thaliana)
- Gamma carbonic anhydrase 2, mitochondrial (30 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (53 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (10 kDa, Protein from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 4 (55 kDa, Protein from Arabidopsis thaliana)
- Phosphatidylinositol (887 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Arabidopsis thaliana)
- At3g07480.1 (17 kDa, Protein from Arabidopsis thaliana)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Acyl carrier protein 2, mitochondrial (14 kDa, Protein from Arabidopsis thaliana)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Uncharacterized protein at2g27730, mitochondrial (11 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (11 kDa, Protein from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 5 (74 kDa, Protein from Arabidopsis thaliana)
- Fe (iii) ion (55 Da, Ligand)
- P1 (11 kDa, Protein from Arabidopsis thaliana)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3 (22 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (81 kDa, Protein from Arabidopsis thaliana)
- Esss subunit of nadh:ubiquinone oxidoreductase (complex i) protein (12 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (28 kDa, Protein from Arabidopsis thaliana)
- At2g46540/f11c10.23 (6 kDa, Protein from Arabidopsis thaliana)
- Unknown (2 kDa, Protein from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 1 (36 kDa, Protein from Arabidopsis thaliana)
- Acyl carrier protein 1, mitochondrial (13 kDa, Protein from Arabidopsis thaliana)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8-a, mitochondrial (25 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (43 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-b (11 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5-b (9 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (7 kDa, Protein from Arabidopsis thaliana)
- B15 -- 1 beta subcomplex subunit 4 (8 kDa, Protein from Arabidopsis thaliana)
Cryo-EM structure of Arabidopsis thaliana Complex-I (membrane core)
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 7aqq
Q-score: 0.549
Klusch N, Senkler J, Yildiz O, Kuhlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ DOI: doi:10.1093/plcell/koab092 Pubmed: 33768254 ]
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- At4g16450 (11 kDa, Protein from Arabidopsis thaliana)
- Gamma carbonic anhydrase-like 2, mitochondrial (27 kDa, Protein from Arabidopsis thaliana)
- 1,2-dicaproyl-sn-phosphatidyl-l-serine (455 Da, Ligand)
- Arabidopsis complex i - membrane core (Complex from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 2 (55 kDa, Protein from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 6 (23 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-a (16 kDa, Protein from Arabidopsis thaliana)
- Gamma carbonic anhydrase 1, mitochondrial (30 kDa, Protein from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 4 (56 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase subunit 4l (11 kDa, Protein from Arabidopsis thaliana)
- Phosphatidylethanolamine (734 Da, Ligand)
- Excitatory amino acid transporter (9 kDa, Protein from Arabidopsis thaliana)
- Ubiquinone-9 (795 Da, Ligand)
- Gamma carbonic anhydrase 2, mitochondrial (30 kDa, Protein from Arabidopsis thaliana)
- Phosphatidylinositol (887 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Arabidopsis thaliana)
- At3g07480.1 (17 kDa, Protein from Arabidopsis thaliana)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Uncharacterized protein at2g27730, mitochondrial (11 kDa, Protein from Arabidopsis thaliana)
- Fe (iii) ion (55 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (74 kDa, Protein from Arabidopsis thaliana)
- P1 (11 kDa, Protein from Arabidopsis thaliana)
- At2g46540/f11c10.23 (6 kDa, Protein from Arabidopsis thaliana)
- Unknown (2 kDa, Protein from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 1 (36 kDa, Protein from Arabidopsis thaliana)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5-b (9 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-b (11 kDa, Protein from Arabidopsis thaliana)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (7 kDa, Protein from Arabidopsis thaliana)