Cryo-EM structure of Euglena gracilis super-complex I+III2+IV, composite
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8iuf
Q-score: 0.615
He Z, Wu M, Tian H, Wang L, Hu Y, Han F, Zhou J, Wang Y, Zhou L
Nat Commun (2024) 15 pp. 1628-1628 [ DOI: doi:10.1038/s41467-024-46018-z Pubmed: 38388527 ]
- Cytochrome c oxidase subunit 2 (22 kDa, Protein from Euglena gracilis)
- Cox7c (19 kDa, Protein from Euglena gracilis)
- Nduca1 (48 kDa, Protein from Euglena gracilis)
- Ndufs2 (45 kDa, Protein from Euglena gracilis)
- Ndueg3 (63 kDa, Protein from Euglena gracilis)
- Uqcrq (9 kDa, Protein from Euglena gracilis)
- Ndufa2 (21 kDa, Protein from Euglena gracilis)
- Coxeg6 (8 kDa, Protein from Euglena gracilis)
- Ndueg9 (18 kDa, Protein from Euglena gracilis)
- Ndufb7 (12 kDa, Protein from Euglena gracilis)
- Nd1 (79 kDa, Protein from Euglena gracilis)
- Ndufb3 (6 kDa, Protein from Euglena gracilis)
- Ubiquinone-10 (863 Da, Ligand)
- Uqcr9 (6 kDa, Protein from Euglena gracilis)
- Ndufs5 (14 kDa, Protein from Euglena gracilis)
- Nd2a (35 kDa, Protein from Euglena gracilis)
- Ndufa3 (14 kDa, Protein from Euglena gracilis)
- Heme c (618 Da, Ligand)
- Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial (51 kDa, Protein from Euglena gracilis)
- Ndufb2 (17 kDa, Protein from Euglena gracilis)
- Coxeg9 (31 kDa, Protein from Euglena gracilis)
- Nadh-ubiquinone oxidoreductase chain 4 (56 kDa, Protein from Euglena gracilis)
- Cytochrome b (42 kDa, Protein from Euglena gracilis)
- Iron/sulfur cluster (351 Da, Ligand)
- Nd2b (15 kDa, Protein from Euglena gracilis)
- Ubiquinol-cytochrome-c reductase complex subunit ix, mitochondrial (11 kDa, Protein from Euglena gracilis)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Putative nadh dehydrogenase subunit 6 (19 kDa, Protein from Euglena gracilis)
- Ndufs7 (22 kDa, Protein from Euglena gracilis)
- Cox5c (23 kDa, Protein from Euglena gracilis)
- Ndufs1b (59 kDa, Protein from Euglena gracilis)
- Coxeg5 (19 kDa, Protein from Euglena gracilis)
- Ndufa5 (20 kDa, Protein from Euglena gracilis)
- O-[(s)-hydroxy{[(2s)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-l-serine (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Ndufa7 (16 kDa, Protein from Euglena gracilis)
- Ndufab1-beta (14 kDa, Protein from Euglena gracilis)
- Ndueg5 (29 kDa, Protein from Euglena gracilis)
- Ndufb9 (18 kDa, Protein from Euglena gracilis)
- Coxeg8 (24 kDa, Protein from Euglena gracilis)
- Zinc ion (65 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nd4l (19 kDa, Protein from Euglena gracilis)
- Ndufa6 (48 kDa, Protein from Euglena gracilis)
- Poly(unk) (1 kDa, Protein from Euglena gracilis)
- Ndufa1 (15 kDa, Protein from Euglena gracilis)
- Ndufb8 (20 kDa, Protein from Euglena gracilis)
- Cox7a (20 kDa, Protein from Euglena gracilis)
- Ndufx (37 kDa, Protein from Euglena gracilis)
- Ndufb6 (10 kDa, Protein from Euglena gracilis)
- Ndufc2 (22 kDa, Protein from Euglena gracilis)
- Cardiolipin (1 kDa, Ligand)
- Ndufs6 (17 kDa, Protein from Euglena gracilis)
- Cox5b-2 (19 kDa, Protein from Euglena gracilis)
- Ndufa9 (54 kDa, Protein from Euglena gracilis)
- Euglena gracilis respirasome supercomplex i+iii2+iv (Complex from Euglena gracilis)
- Ndueg10 (14 kDa, Protein from Euglena gracilis)
- Ndufb11 (13 kDa, Protein from Euglena gracilis)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nduca3 (28 kDa, Protein from Euglena gracilis)
- Cytochrome c oxidase subunit 1 (55 kDa, Protein from Euglena gracilis)
- Ndueg11 (11 kDa, Protein from Euglena gracilis)
- Nd3 (34 kDa, Protein from Euglena gracilis)
- Ndufs1a (43 kDa, Protein from Euglena gracilis)
- Ndufa8 (25 kDa, Protein from Euglena gracilis)
- Nduca2 (28 kDa, Protein from Euglena gracilis)
- Cox6b-1 (32 kDa, Protein from Euglena gracilis)
- Ndueg7 (26 kDa, Protein from Euglena gracilis)
- Uqcr10 (17 kDa, Protein from Euglena gracilis)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Ndueg2 (51 kDa, Protein from Euglena gracilis)
- Coxeg3 (16 kDa, Protein from Euglena gracilis)
- Ndufs3 (32 kDa, Protein from Euglena gracilis)
- Ndufa11 (32 kDa, Protein from Euglena gracilis)
- 1,2-dilauroyl-sn-glycero-3-phosphate (535 Da, Ligand)
- Cytochrome c1, heme protein (27 kDa, Protein from Euglena gracilis)
- Copper (ii) ion (63 Da, Ligand)
- Coxeg1 (27 kDa, Protein from Euglena gracilis)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Ndueg6 (43 kDa, Protein from Euglena gracilis)
- Cox4 (20 kDa, Protein from Euglena gracilis)
- Ndueg4 (41 kDa, Protein from Euglena gracilis)
- Nd5 (68 kDa, Protein from Euglena gracilis)
- Ndufs8 (24 kDa, Protein from Euglena gracilis)
- Ndufa13 (22 kDa, Protein from Euglena gracilis)
- Potassium ion (39 Da, Ligand)
- Coxeg10 (10 kDa, Protein from Euglena gracilis)
- Euglena gracilis respirasome supercomplex i+iii2+iv (2 MDa, Complex from Euglena gracilis)
- Flavin mononucleotide (456 Da, Ligand)
- Ndufs4 (23 kDa, Protein from Euglena gracilis)
- Ndueg8 (23 kDa, Protein from Euglena gracilis)
- Coxeg7 (35 kDa, Protein from Euglena gracilis)
- Ndufv2 (25 kDa, Protein from Euglena gracilis)
- Ndueg13 (17 kDa, Protein from Euglena gracilis)
- Ndufv1 (57 kDa, Protein from Euglena gracilis)
- Uqcrfs1 (27 kDa, Protein from Euglena gracilis)
- Uqcrh (8 kDa, Protein from Euglena gracilis)
- Ndufab1-alpha (14 kDa, Protein from Euglena gracilis)
- Uqcrb (27 kDa, Protein from Euglena gracilis)
- Ndufa12 (33 kDa, Protein from Euglena gracilis)
- Ndueg1 (53 kDa, Protein from Euglena gracilis)
- Heme-a (852 Da, Ligand)
- Mpp-beta (53 kDa, Protein from Euglena gracilis)
- Coxeg4 (19 kDa, Protein from Euglena gracilis)
- Ndufb5 (15 kDa, Protein from Euglena gracilis)
- Ndufb10 (17 kDa, Protein from Euglena gracilis)
- Cox6a (13 kDa, Protein from Euglena gracilis)
- Ndufb4 (19 kDa, Protein from Euglena gracilis)
- Ndueg11 (11 kDa, Protein from Euglena gracilis)
Cryo-EM composite map of Euglena gracilis respiratory complex I, deactive state
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 8j9h
Q-score: 0.528
He Z, Wu M, Tian H, Wang L, Hu Y, Han F, Zhou J, Wang Y, Zhou L
Nat Commun (2024) 15 pp. 1628-1628 [ DOI: doi:10.1038/s41467-024-46018-z Pubmed: 38388527 ]
- Cardiolipin (1 kDa, Ligand)
- Ndueg3 (46 kDa, Protein from Euglena gracilis)
- Nduca1 (48 kDa, Protein from Euglena gracilis)
- Ndufs2 (45 kDa, Protein from Euglena gracilis)
- Ndufa9 (54 kDa, Protein from Euglena gracilis)
- Ndufa13 (22 kDa, Protein from Euglena gracilis)
- Ndueg10 (14 kDa, Protein from Euglena gracilis)
- Ndufs6 (17 kDa, Protein from Euglena gracilis)
- Ndufs7 (22 kDa, Protein from Euglena gracilis)
- Ndufs8 (24 kDa, Protein from Euglena gracilis)
- Potassium ion (39 Da, Ligand)
- Ndufb11 (13 kDa, Protein from Euglena gracilis)
- Ndufa5 (20 kDa, Protein from Euglena gracilis)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Euglena gracilis respiratory complex i, deactive state (1 MDa, Complex from Euglena gracilis)
- Flavin mononucleotide (456 Da, Ligand)
- Ndueg2 (50 kDa, Protein from Euglena gracilis)
- Ndufs4 (23 kDa, Protein from Euglena gracilis)
- Ndueg8 (23 kDa, Protein from Euglena gracilis)
- Nduca3 (28 kDa, Protein from Euglena gracilis)
- Ndufv2 (25 kDa, Protein from Euglena gracilis)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Ndueg13 (17 kDa, Protein from Euglena gracilis)
- Ndufa2 (21 kDa, Protein from Euglena gracilis)
- Nd3 (34 kDa, Protein from Euglena gracilis)
- Ndufv1 (57 kDa, Protein from Euglena gracilis)
- Ndufa7 (16 kDa, Protein from Euglena gracilis)
- Ndufab1-beta (14 kDa, Protein from Euglena gracilis)
- Ndufa8 (25 kDa, Protein from Euglena gracilis)
- Ndueg5 (29 kDa, Protein from Euglena gracilis)
- Ndufb7 (12 kDa, Protein from Euglena gracilis)
- Nduca2 (28 kDa, Protein from Euglena gracilis)
- Ndufb9 (18 kDa, Protein from Euglena gracilis)
- Ndus1a (43 kDa, Protein from Euglena gracilis)
- Nd1 (79 kDa, Protein from Euglena gracilis)
- Ndueg7 (26 kDa, Protein from Euglena gracilis)
- Ndufb3 (6 kDa, Protein from Euglena gracilis)
- Ubiquinone-10 (863 Da, Ligand)
- Ndus1b (59 kDa, Protein from Euglena gracilis)
- Ndufab1-alpha (14 kDa, Protein from Euglena gracilis)
- Ndufa12 (33 kDa, Protein from Euglena gracilis)
- Ndueg1 (53 kDa, Protein from Euglena gracilis)
- Ndufs5 (14 kDa, Protein from Euglena gracilis)
- Zinc ion (65 Da, Ligand)
- Nd5 (68 kDa, Protein from Euglena gracilis)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Ndufa3 (14 kDa, Protein from Euglena gracilis)
- Ndufs3 (32 kDa, Protein from Euglena gracilis)
- Ndufb5 (15 kDa, Protein from Euglena gracilis)
- Ndufa6 (48 kDa, Protein from Euglena gracilis)
- Nd4l (19 kDa, Protein from Euglena gracilis)
- Ndufa11 (32 kDa, Protein from Euglena gracilis)
- Nd2a (35 kDa, Protein from Euglena gracilis)
- Ndufb2 (17 kDa, Protein from Euglena gracilis)
- Ndufa1 (15 kDa, Protein from Euglena gracilis)
- Ndufb8 (20 kDa, Protein from Euglena gracilis)
- Ndufb10 (17 kDa, Protein from Euglena gracilis)
- Ndueg12 (11 kDa, Protein from Euglena gracilis)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (56 kDa, Protein from Euglena gracilis)
- Unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk (1 kDa, Protein from Euglena gracilis)
- Iron/sulfur cluster (351 Da, Ligand)
- Ndueg6 (43 kDa, Protein from Euglena gracilis)
- Ndufx (37 kDa, Protein from Euglena gracilis)
- Ndufb4 (19 kDa, Protein from Euglena gracilis)
- Ndufb6 (10 kDa, Protein from Euglena gracilis)
- Ndufc2 (22 kDa, Protein from Euglena gracilis)
- Ndueg4 (41 kDa, Protein from Euglena gracilis)
- Nadh dehydrogenase subunit 5 (15 kDa, Protein from Euglena gracilis)
- Ndueg11 (11 kDa, Protein from Euglena gracilis)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Cryo-EM composite map of Euglena gracilis complex I, turnover state
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8j9i
Q-score: 0.584
He Z, Wu M, Tian H, Wang L, Hu Y, Han F, Zhou J, Wang Y, Zhou L
Nat Commun (2024) 15 pp. 1628-1628 [ DOI: doi:10.1038/s41467-024-46018-z Pubmed: 38388527 ]
- Cardiolipin (1 kDa, Ligand)
- Ndueg3 (46 kDa, Protein from Euglena gracilis)
- Nduca1 (48 kDa, Protein from Euglena gracilis)
- Ndufs2 (45 kDa, Protein from Euglena gracilis)
- Ndufa9 (54 kDa, Protein from Euglena gracilis)
- Ndufa13 (22 kDa, Protein from Euglena gracilis)
- Ndueg10 (14 kDa, Protein from Euglena gracilis)
- Ndufs6 (17 kDa, Protein from Euglena gracilis)
- Ndufs7 (22 kDa, Protein from Euglena gracilis)
- Ndufs8 (24 kDa, Protein from Euglena gracilis)
- Potassium ion (39 Da, Ligand)
- Ndufb11 (13 kDa, Protein from Euglena gracilis)
- Ndufa5 (20 kDa, Protein from Euglena gracilis)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Ndufs1a (43 kDa, Protein from Euglena gracilis)
- Flavin mononucleotide (456 Da, Ligand)
- Ndueg2 (50 kDa, Protein from Euglena gracilis)
- Ndufs4 (23 kDa, Protein from Euglena gracilis)
- Ndueg8 (23 kDa, Protein from Euglena gracilis)
- Nduca3 (28 kDa, Protein from Euglena gracilis)
- Ndufv2 (25 kDa, Protein from Euglena gracilis)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Ndueg13 (17 kDa, Protein from Euglena gracilis)
- Ndufa2 (21 kDa, Protein from Euglena gracilis)
- Nd3 (34 kDa, Protein from Euglena gracilis)
- Ndufv1 (57 kDa, Protein from Euglena gracilis)
- Ndufa7 (16 kDa, Protein from Euglena gracilis)
- Ndufab1-beta (14 kDa, Protein from Euglena gracilis)
- Nd2b (15 kDa, Protein from Euglena gracilis)
- Ndufa8 (25 kDa, Protein from Euglena gracilis)
- Ndueg5 (29 kDa, Protein from Euglena gracilis)
- Ndufb7 (12 kDa, Protein from Euglena gracilis)
- Nduca2 (28 kDa, Protein from Euglena gracilis)
- Ndufb9 (18 kDa, Protein from Euglena gracilis)
- Nd1 (79 kDa, Protein from Euglena gracilis)
- Ndueg7 (26 kDa, Protein from Euglena gracilis)
- Ndufb3 (6 kDa, Protein from Euglena gracilis)
- Ubiquinone-10 (863 Da, Ligand)
- Ndufab1-alpha (14 kDa, Protein from Euglena gracilis)
- Ndufa12 (33 kDa, Protein from Euglena gracilis)
- Ndueg1 (53 kDa, Protein from Euglena gracilis)
- Ndufs5 (14 kDa, Protein from Euglena gracilis)
- Zinc ion (65 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Ndufa3 (14 kDa, Protein from Euglena gracilis)
- Ndufs3 (32 kDa, Protein from Euglena gracilis)
- Ndufb5 (15 kDa, Protein from Euglena gracilis)
- Ndufa6 (48 kDa, Protein from Euglena gracilis)
- Nd4l (19 kDa, Protein from Euglena gracilis)
- Ndufa11 (32 kDa, Protein from Euglena gracilis)
- Ndufs1b (59 kDa, Protein from Euglena gracilis)
- Ndufb2 (17 kDa, Protein from Euglena gracilis)
- Nd2a (35 kDa, Protein from Euglena gracilis)
- Euglena gracilis respiratory complex i, turnover state (1 MDa, Complex from Euglena gracilis)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Ndufa1 (15 kDa, Protein from Euglena gracilis)
- Ndufb8 (20 kDa, Protein from Euglena gracilis)
- Ndufb10 (17 kDa, Protein from Euglena gracilis)
- Ndueg12 (11 kDa, Protein from Euglena gracilis)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (56 kDa, Protein from Euglena gracilis)
- Unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk (1 kDa, Protein from Euglena gracilis)
- Iron/sulfur cluster (351 Da, Ligand)
- Ndueg6 (43 kDa, Protein from Euglena gracilis)
- Ndufx (37 kDa, Protein from Euglena gracilis)
- Ndufb4 (19 kDa, Protein from Euglena gracilis)
- Ndufb6 (10 kDa, Protein from Euglena gracilis)
- Ndufc2 (22 kDa, Protein from Euglena gracilis)
- Ndueg4 (41 kDa, Protein from Euglena gracilis)
- Nd5 (68 kDa, Protein from Euglena gracilis)
- Ndueg11 (11 kDa, Protein from Euglena gracilis)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Cryo-EM composite map of Euglena gracilis complex I, NADH state
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8j9j
Q-score: 0.531
He Z, Wu M, Tian H, Wang L, Hu Y, Han F, Zhou J, Wang Y, Zhou L
Nat Commun (2024) 15 pp. 1628-1628 [ DOI: doi:10.1038/s41467-024-46018-z Pubmed: 38388527 ]
- Cardiolipin (1 kDa, Ligand)
- Ndueg3 (46 kDa, Protein from Euglena gracilis)
- Nduca1 (48 kDa, Protein from Euglena gracilis)
- Ndufs2 (45 kDa, Protein from Euglena gracilis)
- Ndufa9 (54 kDa, Protein from Euglena gracilis)
- Ndufa13 (22 kDa, Protein from Euglena gracilis)
- Ndueg10 (14 kDa, Protein from Euglena gracilis)
- Ndufs6 (17 kDa, Protein from Euglena gracilis)
- Ndufs7 (22 kDa, Protein from Euglena gracilis)
- Ndufs8 (24 kDa, Protein from Euglena gracilis)
- Potassium ion (39 Da, Ligand)
- Ndufb11 (13 kDa, Protein from Euglena gracilis)
- Ndufa5 (20 kDa, Protein from Euglena gracilis)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Ndufs1a (43 kDa, Protein from Euglena gracilis)
- Flavin mononucleotide (456 Da, Ligand)
- Ndueg2 (50 kDa, Protein from Euglena gracilis)
- Ndufs4 (23 kDa, Protein from Euglena gracilis)
- Ndueg8 (23 kDa, Protein from Euglena gracilis)
- Nduca3 (28 kDa, Protein from Euglena gracilis)
- Ndufv2 (25 kDa, Protein from Euglena gracilis)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Ndueg13 (17 kDa, Protein from Euglena gracilis)
- Ndufa2 (21 kDa, Protein from Euglena gracilis)
- Nd3 (34 kDa, Protein from Euglena gracilis)
- Ndufv1 (57 kDa, Protein from Euglena gracilis)
- Ndufa7 (16 kDa, Protein from Euglena gracilis)
- Ndufab1-beta (14 kDa, Protein from Euglena gracilis)
- Nd2b (15 kDa, Protein from Euglena gracilis)
- Ndufa8 (25 kDa, Protein from Euglena gracilis)
- Ndueg5 (29 kDa, Protein from Euglena gracilis)
- Ndufb7 (12 kDa, Protein from Euglena gracilis)
- Nduca2 (28 kDa, Protein from Euglena gracilis)
- Ndufb9 (18 kDa, Protein from Euglena gracilis)
- Nd1 (79 kDa, Protein from Euglena gracilis)
- Ndueg7 (26 kDa, Protein from Euglena gracilis)
- Ndufb3 (6 kDa, Protein from Euglena gracilis)
- Ubiquinone-10 (863 Da, Ligand)
- Ndufab1-alpha (14 kDa, Protein from Euglena gracilis)
- Ndufa12 (33 kDa, Protein from Euglena gracilis)
- Ndueg1 (53 kDa, Protein from Euglena gracilis)
- Ndufs5 (14 kDa, Protein from Euglena gracilis)
- Zinc ion (65 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Ndufa3 (14 kDa, Protein from Euglena gracilis)
- Ndufs3 (32 kDa, Protein from Euglena gracilis)
- Ndufb5 (15 kDa, Protein from Euglena gracilis)
- Ndufa6 (48 kDa, Protein from Euglena gracilis)
- Nd4l (19 kDa, Protein from Euglena gracilis)
- Ndufa11 (32 kDa, Protein from Euglena gracilis)
- Ndufs1b (59 kDa, Protein from Euglena gracilis)
- Ndufb2 (17 kDa, Protein from Euglena gracilis)
- Nd2a (35 kDa, Protein from Euglena gracilis)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Ndufa1 (15 kDa, Protein from Euglena gracilis)
- Ndufb8 (20 kDa, Protein from Euglena gracilis)
- Ndufb10 (17 kDa, Protein from Euglena gracilis)
- Ndueg12 (11 kDa, Protein from Euglena gracilis)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Euglena gracilis respiratory complex i, nadh state (1 MDa, Complex from Euglena gracilis)
- Nadh-ubiquinone oxidoreductase chain 4 (56 kDa, Protein from Euglena gracilis)
- Unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk-unk (1 kDa, Protein from Euglena gracilis)
- Iron/sulfur cluster (351 Da, Ligand)
- Ndueg6 (43 kDa, Protein from Euglena gracilis)
- Ndufx (37 kDa, Protein from Euglena gracilis)
- Ndufb4 (19 kDa, Protein from Euglena gracilis)
- Ndufb6 (10 kDa, Protein from Euglena gracilis)
- Ndufc2 (22 kDa, Protein from Euglena gracilis)
- Ndueg4 (41 kDa, Protein from Euglena gracilis)
- Nd5 (68 kDa, Protein from Euglena gracilis)
- Ndueg11 (11 kDa, Protein from Euglena gracilis)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Cryo-EM structure of Polytomella Complex-I (membrane arm)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 7ar9
Q-score: 0.565
Klusch N, Senkler J, Yildiz O, Kuhlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ DOI: doi:10.1093/plcell/koab092 Pubmed: 33768254 ]
- Cardiolipin (1 kDa, Ligand)
- Nuop8 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ashi (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B9 (4 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd3 (17 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mwfe (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Pdsw (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd4l (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14.7 (22 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B12 (6 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Pgiv (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Unknown (4 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop5 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- C1-fdx (23 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14.5b (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd2 (41 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Phosphatidylethanolamine (734 Da, Ligand)
- Polytomella complex i - membrane arm (Complex from Polytomella sp. Pringsheim 198.80)
- Mnll (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ca2 (33 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop7 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Sdap1 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop4 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Cal (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B15 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ca3 (24 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Water (18 Da, Ligand)
- Esss (19 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd4 (48 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Aggg (7 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd5 (58 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B22 (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Unknown (3 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 15 kda (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd1 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B16.6 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B18 (10 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Kfyi (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Cryo-EM structure of Polytomella Complex-I (complete composition)
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 7ard
Q-score: 0.557
Klusch N, Senkler J, Yildiz O, Kuhlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ DOI: doi:10.1093/plcell/koab092 Pubmed: 33768254 ]
- B13 (17 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Cardiolipin (1 kDa, Ligand)
- 13 kda (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop8 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ashi (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B9 (4 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd3 (17 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mwfe (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 51 kda (51 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Pdsw (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd4l (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Psst (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Flavin mononucleotide (456 Da, Ligand)
- B14.7 (22 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B12 (6 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Pgiv (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Unknown (4 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14.5a (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop5 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- C1-fdx (23 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14.5b (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd2 (41 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Phosphatidylethanolamine (734 Da, Ligand)
- Cryo-em structure of polytomella complex-i (complete composition) (Complex from Polytomella sp. Pringsheim 198.80)
- B14 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B17.2 (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd7 (45 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mnll (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ca2 (33 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Tyky (26 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop7 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Sdap1 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop4 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Zinc ion (65 Da, Ligand)
- B8 (10 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Complex i-b22 (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Cal (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B15 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Ca3 (24 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- 18 kda (20 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd9 (25 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- 75 kda (77 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Esss (19 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd4 (48 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Aggg (7 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd5 (58 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 39 kda (41 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Sdap2 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 24 kda (30 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Unknown (3 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 15 kda (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd1 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B16.6 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B18 (10 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Kfyi (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
Cryo-EM structure of respiratory complex I from Yarrowia lipolytica
Resolution: 4.32 Å
EM Method: Single-particle
Fitted PDBs: 6gcs
Q-score: 0.371
Parey K, Brandt U, Xie H, Mills DJ, Siegmund K, Vonck J, Kuehlbrandt W, Zickermann V
eLife (2018) 7 [ DOI: doi:10.7554/eLife.39213 Pubmed: 30277212 ]
- Tyky subunit (nuim) (25 kDa, Protein from Yarrowia lipolytica)
- Nuym subunit (18 kDa, Protein from Yarrowia lipolytica)
- Cardiolipin (1 kDa, Ligand)
- Nidm subunit (11 kDa, Protein from Yarrowia lipolytica)
- Ni8m subunit (9 kDa, Protein from Yarrowia lipolytica)
- 24-kda subunit (nuhm) (27 kDa, Protein from Yarrowia lipolytica)
- Nd4 subunit (nu4m) (54 kDa, Protein from Yarrowia lipolytica)
- Nd4l subunit (nulm) (9 kDa, Protein from Yarrowia lipolytica)
- 75-kda protein (nuam) (79 kDa, Protein from Yarrowia lipolytica)
- Nuzm subunit (11 kDa, Protein from Yarrowia lipolytica)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Unknown subunit (4 kDa, Protein from Yarrowia lipolytica)
- Nesm subunit (13 kDa, Protein from Yarrowia lipolytica)
- Nd5 subunit (nu5m) (73 kDa, Protein from Yarrowia lipolytica)
- Acpm2 subunit (14 kDa, Protein from Yarrowia lipolytica)
- Niam subunit (8 kDa, Protein from Yarrowia lipolytica)
- Nb2m subunit (6 kDa, Protein from Yarrowia lipolytica)
- Nd1 subunit (nu1m) (38 kDa, Protein from Yarrowia lipolytica)
- Nd2 subunit (nu2m) (53 kDa, Protein from Yarrowia lipolytica)
- Nd3 subunit (nu3m) (14 kDa, Protein from Yarrowia lipolytica)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- 51-kda protein (nubm) (53 kDa, Protein from Yarrowia lipolytica)
- Nupm subunit (19 kDa, Protein from Yarrowia lipolytica)
- Nd6 subunit (nu6m) (20 kDa, Protein from Yarrowia lipolytica)
- Nb5m subunit (10 kDa, Protein from Yarrowia lipolytica)
- Mitochondrial nadh:ubiquinone oxidoreductase (963 MDa, Complex from Yarrowia lipolytica)
- Ni2m subunit (12 kDa, Protein from Yarrowia lipolytica)
- Nuem subunit (42 kDa, Protein from Yarrowia lipolytica)
- Nimm subunit (9 kDa, Protein from Yarrowia lipolytica)
- 49-kda protein (nucm) (52 kDa, Protein from Yarrowia lipolytica)
- Nb4m subunit (14 kDa, Protein from Yarrowia lipolytica)
- Nb6m subunit (14 kDa, Protein from Yarrowia lipolytica)
- Numm subunit (15 kDa, Protein from Yarrowia lipolytica)
- Nuxm subunit (18 kDa, Protein from Yarrowia lipolytica)
- N7bm subunit (16 kDa, Protein from Yarrowia lipolytica)
- Zinc ion (65 Da, Ligand)
- Nuum subunit (3 kDa, Protein from Yarrowia lipolytica)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Psst subunit (nukm) (23 kDa, Protein from Yarrowia lipolytica)
- Nb8m subunit (11 kDa, Protein from Yarrowia lipolytica)
- Nipm subunit (10 kDa, Protein from Yarrowia lipolytica)
- Nufm subunit (16 kDa, Protein from Yarrowia lipolytica)
- 30-kda protein (nugm) (32 kDa, Protein from Yarrowia lipolytica)
- Ni9m subunit (7 kDa, Protein from Yarrowia lipolytica)
- Iron/sulfur cluster (351 Da, Ligand)
- Nujm subunit (20 kDa, Protein from Yarrowia lipolytica)
- Nunm subunit (7 kDa, Protein from Yarrowia lipolytica)
- Acpm1 subunit (12 kDa, Protein from Yarrowia lipolytica)
- Nebm subunit (5 kDa, Protein from Yarrowia lipolytica)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)