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EMD-14791

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CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM)

Release date: Nov. 30, 2022
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7zm7
Q-score: 0.598

Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W

Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]

Sample:

Nadh-ubiquinone oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Oxidoreductase-like domain-containing protein (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Cardiolipin (1 kDa, Ligand)
Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase (ubiquinone)-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (82 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Water (18 Da, Ligand)
Mitochondrial nadh:ubiquinone oxidoreductase in ddm (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase b14 subunit-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase (ubiquinone)-like protein (43 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Nadh-ubiquinone oxidoreductase 49 kda subunit-like protein (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Zinc ion (65 Da, Ligand)
Nadh-ubiquinone oxidoreductase 30.4 kda subunit-like protein (33 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Flavin mononucleotide (456 Da, Ligand)
Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Iron/sulfur cluster (351 Da, Ligand)
Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufv2 of nadh-ubiquinone oxidoreductase (complex i) (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Dodecyl-beta-d-maltoside (510 Da, Ligand)

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EMD-14792

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CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM) - membrane arm

Release date: Nov. 30, 2022
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7zm8
Q-score: 0.604

Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W

Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]

Sample:

Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Cardiolipin (1 kDa, Ligand)
Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Water (18 Da, Ligand)
Mitochondrial nadh:ubiquinone oxidoreductase in ddm (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh dehydrogenase (ubiquinone)-like protein (89 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Dodecyl-beta-d-maltoside (510 Da, Ligand)

[Show more]

EMD-14794

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CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2)

Release date: Nov. 30, 2022
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7zmb
Q-score: 0.598

Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W

Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]

Sample:

Nadh-ubiquinone oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Oxidoreductase-like domain-containing protein (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Cardiolipin (1 kDa, Ligand)
Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Lauryl maltose neopentyl glycol (1 kDa, Ligand)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase (ubiquinone)-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (82 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Water (18 Da, Ligand)
Nadh-ubiquinone oxidoreductase b14 subunit-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase (ubiquinone)-like protein (43 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Nadh-ubiquinone oxidoreductase 49 kda subunit-like protein (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Zinc ion (65 Da, Ligand)
Nadh-ubiquinone oxidoreductase 30.4 kda subunit-like protein (33 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Flavin mononucleotide (456 Da, Ligand)
Mitochondrial nadh:ubiquinone oxidoreductase in lmng (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Iron/sulfur cluster (351 Da, Ligand)
Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufv2 of nadh-ubiquinone oxidoreductase (complex i) (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)

[Show more]

EMD-14796

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CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2) - membrane arm

Release date: Nov. 30, 2022
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7zme
Q-score: 0.596

Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W

Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]

Sample:

Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Cardiolipin (1 kDa, Ligand)
Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Lauryl maltose neopentyl glycol (1 kDa, Ligand)
Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Water (18 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Mitochondrial nadh:ubiquinone oxidoreductase in lmng (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase (ubiquinone)-like protein (89 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)

[Show more]

EMD-14797

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CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1)

Release date: Nov. 30, 2022
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7zmg
Q-score: 0.667

Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W

Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]

Sample:

Nadh-ubiquinone oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Oxidoreductase-like domain-containing protein (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Cardiolipin (1 kDa, Ligand)
Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Lauryl maltose neopentyl glycol (1 kDa, Ligand)
Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase (ubiquinone)-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (82 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Water (18 Da, Ligand)
Nadh-ubiquinone oxidoreductase b14 subunit-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase (ubiquinone)-like protein (43 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
Nadh-ubiquinone oxidoreductase 49 kda subunit-like protein (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Zinc ion (65 Da, Ligand)
Nadh-ubiquinone oxidoreductase 30.4 kda subunit-like protein (33 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Flavin mononucleotide (456 Da, Ligand)
Mitochondrial nadh:ubiquinone oxidoreductase in lmng (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Iron/sulfur cluster (351 Da, Ligand)
Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufv2 of nadh-ubiquinone oxidoreductase (complex i) (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)

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EMD-14798

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CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1) - membrane arm

Release date: Nov. 30, 2022
Resolution: 2.47 Å
EM Method: Single-particle
Fitted PDBs: 7zmh
Q-score: 0.672

Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W

Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]

Sample:

Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Cardiolipin (1 kDa, Ligand)
Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
Lauryl maltose neopentyl glycol (1 kDa, Ligand)
Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Water (18 Da, Ligand)
Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
Mitochondrial nadh:ubiquinone oxidoreductase in lmng (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh dehydrogenase (ubiquinone)-like protein (89 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)

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