Complex of NPR1 ectodomain with ANP plus an allosteric activating antibody, REGN5381
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8tg9
Q-score: 0.495
Dunn ME, Kithcart A, Kim JH, Ho AJ, Franklin MC, Romero Hernandez A, de Hoon J, Botermans W, Meyer J, Jin X, Zhang D, Torello J, Jasewicz D, Kamat V, Garnova E, Liu N, Rosconi M, Pan H, Karnik S, Burczynski ME, Zheng W, Rafique A, Nielsen JB, De T, Verweij N, Pandit A, Locke A, Chalasani N, Melander O, Schwantes-An TH, Baras A, Lotta LA, Musser BJ, Mastaitis J, Devalaraja-Narashimha KB, Rankin AJ, Huang T, Herman G, Olson W, Murphy AJ, Yancopoulos GD, Olenchock BA, Morton L
Nature (2024) 633 pp. 654-661 [ DOI: doi:10.1038/s41586-024-07903-1 Pubmed: 39261724 ]
- Regn5381 fab heavy chain (24 kDa, Protein from Mus musculus)
- Atrial natriuretic peptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Regn5381 fab light chain (23 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Active complex of npr1 ectodomain, anp, and regn5381 fab (Complex from Homo sapiens)
- Atrial natriuretic peptide (3 kDa, Protein from Homo sapiens)
Complex of NPR1 ectodomain and REGN5381 Fab in an active-like state with no ANP bound
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 8tga
Q-score: 0.117
Dunn ME, Kithcart A, Kim JH, Ho AJ, Franklin MC, Romero Hernandez A, de Hoon J, Botermans W, Meyer J, Jin X, Zhang D, Torello J, Jasewicz D, Kamat V, Garnova E, Liu N, Rosconi M, Pan H, Karnik S, Burczynski ME, Zheng W, Rafique A, Nielsen JB, De T, Verweij N, Pandit A, Locke A, Chalasani N, Melander O, Schwantes-An TH, Baras A, Lotta LA, Musser BJ, Mastaitis J, Devalaraja-Narashimha KB, Rankin AJ, Huang T, Herman G, Olson W, Murphy AJ, Yancopoulos GD, Olenchock BA, Morton L
Nature (2024) 633 pp. 654-661 [ DOI: doi:10.1038/s41586-024-07903-1 Pubmed: 39261724 ]
- Active-like complex of npr1 ectodomain and regn5381 fab, with no anp present (Complex from Homo sapiens)
- Regn5381 fab heavy chain (24 kDa, Protein from Mus musculus)
- Regn5381 fab light chain (23 kDa, Protein from Mus musculus)
- Atrial natriuretic peptide receptor 1 (52 kDa, Protein from Homo sapiens)
Cryo-EM maps of the (TGA3)2-(NPR1)2-(TGA3)2 complex
Kumar S, Zavaliev R, Wu Q, Zhou Y, Cheng J, Dillard L, Powers J, Withers J, Zhao J, Guan Z, Borgnia MJ, Bartesaghi A, Dong X, Zhou P
Nature (2022) 605 pp. 561-566 [ Pubmed: 35545668 DOI: doi:10.1038/s41586-022-04699-w ]
cryo-EM maps of the (NPR1)2-(TGA3)2 complex
Kumar S, Zavaliev R, Wu Q, Zhou Y, Cheng J, Dillard L, Powers J, Withers J, Zhao J, Guan Z, Borgnia MJ, Bartesaghi A, Dong X, Zhou P
Nature (2022) 605 pp. 561-566 [ Pubmed: 35545668 DOI: doi:10.1038/s41586-022-04699-w ]
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7mk2
Q-score: 0.453
Kumar S, Zavaliev R, Wu Q, Zhou Y, Cheng J, Dillard L, Powers J, Withers J, Zhao J, Guan Z, Borgnia MJ, Bartesaghi A, Dong X, Zhou P
Nature (2022) 605 pp. 561-566 [ Pubmed: 35545668 DOI: doi:10.1038/s41586-022-04699-w ]
- Npr1 dimer (Complex from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Regulatory protein npr1 (67 kDa, Protein from Arabidopsis thaliana)
CryoEM Map of the NPR1-SA complex
Kumar S, Zavaliev R, Wu Q, Zhou Y, Cheng J, Dillard L, Powers J, Withers J, Zhao J, Guan Z, Borgnia MJ, Bartesaghi A, Dong X, Zhou P
Nature (2022) 605 pp. 561-566 [ Pubmed: 35545668 DOI: doi:10.1038/s41586-022-04699-w ]
Cryo-EM structure of the (TGA3)2-(NPR1)2-(TGA3)2 complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7tac
Q-score: 0.329
Kumar S, Zavaliev R, Wu Q, Zhou Y, Cheng J, Dillard L, Powers J, Withers J, Zhao J, Guan Z, Borgnia MJ, Bartesaghi A, Dong X, Zhou P
Nature (2022) 605 pp. 561-566 [ Pubmed: 35545668 DOI: doi:10.1038/s41586-022-04699-w ]
- (tga3)2-(npr1)2-(tga3)2 complex (Complex from Arabidopsis thaliana)
- Palmitic acid (256 Da, Ligand)
- Regulatory protein npr1 (67 kDa, Protein from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Transcription factor tga3 (36 kDa, Protein from Arabidopsis thaliana)
CryoEM structure of the (NPR1)2-(TGA3)2 complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7tad
Q-score: 0.357
Kumar S, Zavaliev R, Wu Q, Zhou Y, Cheng J, Dillard L, Powers J, Withers J, Zhao J, Guan Z, Borgnia MJ, Bartesaghi A, Dong X, Zhou P
Nature (2022) 605 pp. 561-566 [ Pubmed: 35545668 DOI: doi:10.1038/s41586-022-04699-w ]
- Palmitic acid (256 Da, Ligand)
- Regulatory protein npr1 (67 kDa, Protein from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- (npr1)2-(tga3)2 complex (Complex from Arabidopsis thaliana)
- Transcription factor tga3 (36 kDa, Protein from Arabidopsis thaliana)