CryoEM Structure of Inactive H2R Bound to Famotidine, Nb6M, and NabFab
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ul3
Q-score: 0.36
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6m (14 kDa, Protein from synthetic construct)
- H2r (Complex from Homo sapiens)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Famotidine (339 Da, Ligand)
- Nb6m (Complex from Lama glama)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nabfab (Complex from synthetic construct)
- Histamine h2 receptor (44 kDa, Protein from Homo sapiens)
- Complex of h2r, nb6m, and nabfab (Complex from Homo sapiens)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7php
Q-score: 0.505
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Multidrug resistance protein norm (52 kDa, Protein from Vibrio cholerae RC385)
- Norm-nb17_4 (14 kDa, Protein from synthetic construct)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Multidrug resistance protein norm (Complex from synthetic construct)
- Nabfab lc (Complex from synthetic construct)
- Norm-nb17_4 (Complex from Lama glama)
- Anti-fab nanobody (Complex)
- Nabfab hc (Complex from synthetic construct)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
Resolution: 8.45 Å
EM Method: Single-particle
Fitted PDBs: 7phq
Q-score: 0.111
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Dmt-nb16_4 (14 kDa, Protein from synthetic construct)
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 7pij
Q-score: 0.422
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Water (18 Da, Ligand)
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Dmtnb16_4 (14 kDa, Protein from synthetic construct)