Cryo-EM structure of pre-consensus elemental paused elongation complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8eg7
Q-score: 0.504
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Pre-consensus elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Rna (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Cryo-EM structure of consensus elemental paused elongation complex with a folded TL
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8eg8
Q-score: 0.507
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Magnesium ion (24 Da, Ligand)
- Rna (5 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Consensus elemental paused elongation complex with a folded tl (450 kDa, Complex from Escherichia coli)
Cryo-EM structure of consensus elemental paused elongation complex with an unfolded TL
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8egb
Q-score: 0.434
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Consensus elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8eh8
Q-score: 0.496
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8eh9
Q-score: 0.429
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8eha
Q-score: 0.461
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (1)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8ehf
Q-score: 0.494
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- (3r,5s,7r,8r,9s,10s,12s,13r,14s,17r)-10,13-dimethyl-17-[(2r)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triol (378 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (2)
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 8ehi
Q-score: 0.195
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
cryo-EM structure of HK022 putRNA-associated E.coli RNA polymerase elongation complex
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 7xue
Q-score: 0.476
Hwang S, Olinares PDB, Lee J, Kim J, Chait BT, King RA, Kang JY
Nat Commun (2022) 13 pp. 4668-4668 [ Pubmed: 35970830 DOI: doi:10.1038/s41467-022-32315-y ]
- Rna polymerase (Complex from Escherichia coli (strain K12))
- Non-template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Rna (nun gene and immunity region) (30 kDa, RNA from Shamshuipovirus HK022)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Dna/rna (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Hk022 putrna-associated e.coli rna polymerase elongation complex (440 kDa, Complex)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
cryo-EM structure of HK022 putRNA-less E.coli RNA polymerase elongation complex
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 7xug
Q-score: 0.446
Hwang S, Olinares PDB, Lee J, Kim J, Chait BT, King RA, Kang JY
Nat Commun (2022) 13 pp. 4668-4668 [ Pubmed: 35970830 DOI: doi:10.1038/s41467-022-32315-y ]
- E.coli rna polymerase (Complex from Escherichia coli (strain K12))
- Non-template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli (strain K12))
- Rna (nun gene and immunity region) (30 kDa, RNA from Shamshuipovirus HK022)
- Magnesium ion (24 Da, Ligand)
- Dna/rna (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Hk022 putrna-less e.coli rna polymerase elongation complex (440 kDa, Complex)
- Zinc ion (65 Da, Ligand)
- Template dna (54 kDa, DNA from Shamshuipovirus HK022)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))