Resolution: 3.66 Å
EM Method: Single-particle
Fitted PDBs: 8hih
Q-score: 0.202
Shi J, Feng Z, Xu J, Li F, Zhang Y, Wen A, Wang F, Song Q, Wang L, Cui H, Tong S, Chen P, Zhu Y, Zhao G, Wang S, Feng Y, Lin W
PNAS (2023) 120 pp. e2300282120-e2300282120 [ DOI: doi:10.1073/pnas.2300282120 Pubmed: 37216560 ]
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Rna polymerase sigma factor siga (57 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis transcription initiation complex with transcription factor glnr (Complex from Mycobacterium tuberculosis)
- Template strand dna of amtb promoter (33 kDa, DNA from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Non-template strand dna of amtb promoter (33 kDa, DNA from Mycobacterium tuberculosis)
- Transcriptional regulatory protein (27 kDa, Protein from Mycobacterium tuberculosis H37Rv)
Cryo-EM structure of pre-consensus elemental paused elongation complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8eg7
Q-score: 0.504
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Pre-consensus elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Rna (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Cryo-EM structure of consensus elemental paused elongation complex with a folded TL
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8eg8
Q-score: 0.507
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Magnesium ion (24 Da, Ligand)
- Rna (5 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Consensus elemental paused elongation complex with a folded tl (450 kDa, Complex from Escherichia coli)
Cryo-EM structure of consensus elemental paused elongation complex with an unfolded TL
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8egb
Q-score: 0.434
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Consensus elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8eh8
Q-score: 0.496
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8eh9
Q-score: 0.429
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8eha
Q-score: 0.461
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (1)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8ehf
Q-score: 0.494
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- (3r,5s,7r,8r,9s,10s,12s,13r,14s,17r)-10,13-dimethyl-17-[(2r)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triol (378 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (2)
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 8ehi
Q-score: 0.195
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (6 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
CryoEM structure of SigmaS-transcription initiation complex with activator Crl
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 6omf
Q-score: 0.463
Cartagena AJ, Banta AB, Sathyan N, Ross W, Gourse RL, Campbell EA, Darst SA
PNAS (2019) 116 pp. 18923-18927 [ DOI: doi:10.1073/pnas.1910827116 Pubmed: 31484766 ]
- Dna-directed rna polymerase subunit beta (Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (Protein from Escherichia coli)
- Cryoem structure of sigmas-transcription initiation complex with activator crl (523 kDa, Complex from Salmonella enterica subsp. enterica serovar Typhimurium)
- Dna-directed rna polymerase subunit sigma-s (Protein from Salmonella enterica subsp. enterica serovar Typhimurium)
- Dna-directed rna polymerase subunit alpha (Protein from Escherichia coli)
- Template dna strand (DNA from Escherichia coli)
- Non-template dna strand (DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (Protein from Escherichia coli)
- Crl (Protein from Salmonella enterica subsp. enterica serovar Typhimurium)