Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8sy5
Q-score: 0.552
Oh J, Shan Z, Hoshika S, Xu J, Chong J, Benner SA, Lyumkis D, Wang D
Nat Commun (2023) 14 pp. 8219-8219 [ Pubmed: 38086811 DOI: doi:10.1038/s41467-023-43735-9 ]
- Rna oligomer (2 kDa, RNA from synthetic construct)
- Water (18 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Template single stranded dna (8 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 2-oxo-2-hydroadenosine 5'-(tetrahydrogen triphosphate) (523 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- E. coli rnap transcription elongation complex bound the unnatural ds-btp base pair in the active site (500 kDa, Complex from Escherichia coli)
- Non-template single stranded dna (5 kDa, DNA from synthetic construct)
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8sy6
Q-score: 0.449
Oh J, Shan Z, Hoshika S, Xu J, Chong J, Benner SA, Lyumkis D, Wang D
Nat Commun (2023) 14 pp. 8219-8219 [ DOI: doi:10.1038/s41467-023-43735-9 Pubmed: 38086811 ]
- Template single stranded dna (8 kDa, DNA from synthetic construct)
- Rna oligomer (2 kDa, RNA from synthetic construct)
- E. coli rnap transcription elongation complex bound the unnatural db-utp base pair in the active site (500 kDa, Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Uridine 5'-triphosphate (484 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Non-template single stranded dna (5 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- 2'-deoxyguanosine-5'-monophosphate (347 Da, Ligand)
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8sy7
Q-score: 0.522
Oh J, Shan Z, Hoshika S, Xu J, Chong J, Benner SA, Lyumkis D, Wang D
Nat Commun (2023) 14 pp. 8219-8219 [ Pubmed: 38086811 DOI: doi:10.1038/s41467-023-43735-9 ]
- Rna oligomer (2 kDa, RNA from synthetic construct)
- Water (18 Da, Ligand)
- E. coli rnap transcription elongation complex bound the unnatural db-stp base pair in the active site (500 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Template single stranded dna (8 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- [[(2~{r},3~{s},4~{r},5~{s})-5-(4-azanyl-1-methyl-2-oxidanylidene-pyrimidin-5-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate (497 Da, Ligand)
- Non-template single stranded dna (5 kDa, DNA from synthetic construct)