Cryo-EM structure of Synechocystis sp. PCC 6803 RPitc
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8gzg
Q-score: 0.488
Shen L, Lai G, You L, Shi J, Wu X, Puiu M, Gu Z, Feng Y, Yuzenkova Y, Zhang Y
PNAS (2023) 120 pp. e2219290120-e2219290120 [ DOI: doi:10.1073/pnas.2219290120 Pubmed: 37036976 ]
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(*up*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- The complex of synechocystis sp. pcc 6803 rpitc (500 kDa, Complex from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit gamma (71 kDa, Protein from Synechocystis sp. PCC 6803)
- Template strand dna (20 kDa, DNA from synthetic construct)
- Nontemplate strand dna (20 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Synechocystis sp. PCC 6803)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor siga (50 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta' (145 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta (123 kDa, Protein from Synechocystis sp. PCC 6803)
Cryo-EM structure of Synechocystis sp. PCC 6803 CTP-bound RPitc
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8gzh
Q-score: 0.534
Shen L, Lai G, You L, Shi J, Wu X, Puiu M, Gu Z, Feng Y, Yuzenkova Y, Zhang Y
PNAS (2023) 120 pp. e2219290120-e2219290120 [ DOI: doi:10.1073/pnas.2219290120 Pubmed: 37036976 ]
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(*cp*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit gamma (71 kDa, Protein from Synechocystis sp. PCC 6803)
- Template strand dna (20 kDa, DNA from synthetic construct)
- Nontemplate strand dna (20 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Synechocystis sp. PCC 6803)
- The complex of synechocystis sp. pcc 6803 ctp-bound rpitc (500 kDa, Complex from Synechocystis sp. PCC 6803)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor siga (50 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta' (145 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta (123 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytidine-5'-triphosphate (483 Da, Ligand)
Resolution: 5.0 Å
EM Method: Single-particle
Fitted PDBs: 6m6a
Q-score: 0.256
Shi J, Wen A, Zhao M, Jin S, You L, Shi Y, Dong S, Hua X, Zhang Y, Feng Y
Nucleic Acids Res (2020) 48 pp. 11762-11772 [ Pubmed: 33068413 DOI: doi:10.1093/nar/gkaa904 ]
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Template strand dna (19 kDa, DNA from Thermus thermophilus)
- Transcription-repair-coupling factor (109 kDa, Protein from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Nontemplate strand dna (19 kDa, DNA from Thermus thermophilus)
- Thermus thermophilus transcription-coupled dna repair complex (Complex from Thermus thermophilus)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6m6b
Q-score: 0.357
Shi J, Wen A, Zhao M, Jin S, You L, Shi Y, Dong S, Hua X, Zhang Y, Feng Y
Nucleic Acids Res (2020) 48 pp. 11762-11772 [ Pubmed: 33068413 DOI: doi:10.1093/nar/gkaa904 ]
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Template strand dna (19 kDa, DNA from Thermus thermophilus)
- Transcription-repair-coupling factor (109 kDa, Protein from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Thermus thermophilus transcription-repair coupling complex (Complex from Thermus thermophilus)
- Nontemplate strand dna (19 kDa, DNA from Thermus thermophilus)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
CryoEM structure of Thermus thermophilus RNA polymerase elongation complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6m6c
Q-score: 0.519
Shi J, Wen A, Zhao M, Jin S, You L, Shi Y, Dong S, Hua X, Zhang Y, Feng Y
Nucleic Acids Res (2020) 48 pp. 11762-11772 [ Pubmed: 33068413 DOI: doi:10.1093/nar/gkaa904 ]
- Rna (6 kDa, RNA from Thermus thermophilus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Template strand dna (19 kDa, DNA from Thermus thermophilus)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Thermus thermophilus rna polymerase elongation complex (Complex from Thermus thermophilus)
- Nontemplate strand dna (19 kDa, DNA from Thermus thermophilus)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))