Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6xh7
Q-score: 0.344
Shi W, Zhang B, Jiang Y, Liu C, Zhou W, Chen M, Yang Y, Hu Y, Liu B
iScience (2021) 24 pp. 102449-102449 [ DOI: doi:10.1016/j.isci.2021.102449 Pubmed: 34113812 ]
- Cuer-tac without rna (530 kDa, Complex from Escherichia coli)
- Nontemplate strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Copper (ii) ion (63 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Hth-type transcriptional regulator cuer (16 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Template strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
CueR-transcription activation complex with RNA transcript
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6xh8
Q-score: 0.318
Shi W, Zhang B, Jiang Y, Liu C, Zhou W, Chen M, Yang Y, Hu Y, Liu B
iScience (2021) 24 pp. 102449-102449 [ DOI: doi:10.1016/j.isci.2021.102449 Pubmed: 34113812 ]
- Cuer-tac with rna (540 kDa, Complex from Escherichia coli)
- Nontemplate strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Copper (ii) ion (63 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Hth-type transcriptional regulator cuer (16 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Template strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Nascent rna (1 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Sigm28-transcription initiation complex with specific promoter at the state 1
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 6pmi
Q-score: 0.248
Shi W, Zhou W, Zhang B, Huang S, Jiang Y, Schammel A, Hu Y, Liu B
EMBO J (2020) 39 pp. e104389-e104389 [ DOI: doi:10.15252/embj.2020104389 Pubmed: 32484956 ]
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli O157:H7)
- Rna polymerase sigma factor flia (28 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli O45:K1 (strain S88 / ExPEC))
- Magnesium ion (24 Da, Ligand)
- Sigm28-transcription initiation complex with specific promoter at the state 1 (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli O45:K1 (strain S88 / ExPEC))
- Zinc ion (65 Da, Ligand)
- Synthetic template strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Synthetic nontemplate strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli O157:H7)
- Nascent rna (1 kDa, RNA from Escherichia coli)
Sigm28-transcription initiation complex with specific promoter at the state 2
Resolution: 3.91 Å
EM Method: Single-particle
Fitted PDBs: 6pmj
Q-score: 0.207
Shi W, Zhou W, Zhang B, Huang S, Jiang Y, Schammel A, Hu Y, Liu B
EMBO J (2020) 39 pp. e104389-e104389 [ DOI: doi:10.15252/embj.2020104389 Pubmed: 32484956 ]
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli O157:H7)
- Rna polymerase sigma factor flia (28 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli O45:K1 (strain S88 / ExPEC))
- Magnesium ion (24 Da, Ligand)
- Sigm28-transcription initiation complex with specific promoter at the state 2 (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli O45:K1 (strain S88 / ExPEC))
- Zinc ion (65 Da, Ligand)
- Synthetic template strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Synthetic nontemplate strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli O157:H7)
- Nascent rna (1 kDa, RNA from Escherichia coli)
Resolution: 4.35 Å
EM Method: Single-particle
Fitted PDBs: 6pb4
Q-score: 0.224
Shi W, Jiang Y, Deng Y, Dong Z, Liu B
PLoS Biol (2020) 18 pp. e3000706- [ DOI: doi:10.1371/journal.pbio.3000706 ]
- The e. coli class-ii cap-dependent transcription activation complex with de novo rna transcript at the state 2 (550 kDa, Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna/rna (Complex)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha, dna-directed rna polymerase subunit beta, dna-directed rna polymerase subunit beta', dna-directed rna polymerase subunit omega, rna polymerase sigma factor rpod, camp-activated global transcriptional regulator crp (Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Nascent rna (1 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Synthetic template strand dna (78-mer) (24 kDa, DNA from Escherichia coli)
- Synthetic nontemplate strand dna (78-mer) (23 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli)
The E. coli class-II CAP-dependent transcription activation complex at the state 1 architecture
Resolution: 4.52 Å
EM Method: Single-particle
Fitted PDBs: 6pb5
Q-score: 0.082
Shi W, Jiang Y, Deng Y, Dong Z, Liu B
PLoS Biol (2020) 18 pp. e3000706- [ DOI: doi:10.1371/journal.pbio.3000706 ]
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- The e coli class-ii cap-dependent transcription activation complex at the state 1 (550 kDa, Complex from Escherichia coli)
- Synthetic template strand dna (78-mer) (24 kDa, DNA from Escherichia coli)
- Synthetic nontemplate strand dna (78-mer) (23 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli)
The E. coli class-II CAP-dependent transcription activation complex at the state 2
Resolution: 4.29 Å
EM Method: Single-particle
Fitted PDBs: 6pb6
Q-score: 0.223
Shi W, Jiang Y, Deng Y, Dong Z, Liu B
PLoS Biol (2020) 18 pp. e3000706- [ DOI: doi:10.1371/journal.pbio.3000706 ]
- Magnesium ion (24 Da, Ligand)
- The e. coli class-ii cap-dependent transcription activation complex at the state 2 (550 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha, dna-directed rna polymerase subunit beta, dna-directed rna polymerase subunit beta', dna-directed rna polymerase subunit omega, rna polymerase sigma factor rpod, camp-activated global transcriptional regulator crp (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Synthetic template strand dna (78-mer) (24 kDa, DNA from Escherichia coli)
- Synthetic nontemplate strand dna (78-mer) (23 kDa, DNA from Escherichia coli)
- Dna (Complex)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli)