Cryo-EM structure of Synechocystis sp. PCC 6803 RPitc
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8gzg
Q-score: 0.488
Shen L, Lai G, You L, Shi J, Wu X, Puiu M, Gu Z, Feng Y, Yuzenkova Y, Zhang Y
PNAS (2023) 120 pp. e2219290120-e2219290120 [ DOI: doi:10.1073/pnas.2219290120 Pubmed: 37036976 ]
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(*up*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- The complex of synechocystis sp. pcc 6803 rpitc (500 kDa, Complex from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit gamma (71 kDa, Protein from Synechocystis sp. PCC 6803)
- Template strand dna (20 kDa, DNA from synthetic construct)
- Nontemplate strand dna (20 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Synechocystis sp. PCC 6803)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor siga (50 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta' (145 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta (123 kDa, Protein from Synechocystis sp. PCC 6803)
Cryo-EM structure of Synechocystis sp. PCC 6803 CTP-bound RPitc
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8gzh
Q-score: 0.534
Shen L, Lai G, You L, Shi J, Wu X, Puiu M, Gu Z, Feng Y, Yuzenkova Y, Zhang Y
PNAS (2023) 120 pp. e2219290120-e2219290120 [ DOI: doi:10.1073/pnas.2219290120 Pubmed: 37036976 ]
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(*cp*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit gamma (71 kDa, Protein from Synechocystis sp. PCC 6803)
- Template strand dna (20 kDa, DNA from synthetic construct)
- Nontemplate strand dna (20 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Synechocystis sp. PCC 6803)
- The complex of synechocystis sp. pcc 6803 ctp-bound rpitc (500 kDa, Complex from Synechocystis sp. PCC 6803)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor siga (50 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta' (145 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna-directed rna polymerase subunit beta (123 kDa, Protein from Synechocystis sp. PCC 6803)
- Cytidine-5'-triphosphate (483 Da, Ligand)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6xh7
Q-score: 0.344
Shi W, Zhang B, Jiang Y, Liu C, Zhou W, Chen M, Yang Y, Hu Y, Liu B
iScience (2021) 24 pp. 102449-102449 [ DOI: doi:10.1016/j.isci.2021.102449 Pubmed: 34113812 ]
- Cuer-tac without rna (530 kDa, Complex from Escherichia coli)
- Nontemplate strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Copper (ii) ion (63 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Hth-type transcriptional regulator cuer (16 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Template strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
CueR-transcription activation complex with RNA transcript
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6xh8
Q-score: 0.318
Shi W, Zhang B, Jiang Y, Liu C, Zhou W, Chen M, Yang Y, Hu Y, Liu B
iScience (2021) 24 pp. 102449-102449 [ DOI: doi:10.1016/j.isci.2021.102449 Pubmed: 34113812 ]
- Cuer-tac with rna (540 kDa, Complex from Escherichia coli)
- Nontemplate strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Copper (ii) ion (63 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Hth-type transcriptional regulator cuer (16 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Template strand dna (54-mer) (16 kDa, DNA from Escherichia coli)
- Nascent rna (1 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
The cryo-EM structure of a bacterial class I transcription activation complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6b6h
Q-score: 0.283
Liu B, Hong C, Huang RK, Yu Z, Steitz TA
Science (2017) 358 pp. 947-951 [ DOI: doi:10.1126/science.aao1923 Pubmed: 29146813 ]
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli O157:H7)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli (strain K12))
- Synthetic nontemplate strand dna (88-mer) (27 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (8 kDa, Protein from Escherichia coli O157:H7)
- Synthetic template strand dna (88-mer) (27 kDa, DNA from Escherichia coli)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli O45:K1 (strain S88 / ExPEC))
- Zinc ion (65 Da, Ligand)
- The complex of class-i bacterial transcription activation complex (590 kDa, Cellular component from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli O45:K1)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli O157:H7)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli O157:H7)
- Nascent rna 3-mer (1 kDa, RNA from Escherichia coli)