Structure of human oligosaccharyltransferase OST-A complex bound to NGI-1
Resolution: 3.61 Å
EM Method: Single-particle
Fitted PDBs: 8pn9
Q-score: 0.474
Lampson BL, Ramirez AS, Baro M, He L, Hegde M, Koduri V, Pfaff JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, Locher KP, Kaelin Jr WG
Cell (2024) 187 pp. 2209-2223.e16 [ DOI: doi:10.1016/j.cell.2024.03.022 Pubmed: 38670073 ]
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 (4 kDa, Protein from Homo sapiens)
- Transmembrane protein 258 (9 kDa, Protein from Homo sapiens)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (69 kDa, Protein from Homo sapiens)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kda subunit (50 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Oligosaccharyltransferase complex ost-a with bound ngi-1 inhibitor (Complex from Homo sapiens)
- (4r,7r)-4-hydroxy-n,n,n-trimethyl-4,9-dioxo-7-[(undecanoyloxy)methyl]-3,5,8-trioxa-4lambda~5~-phosphadocosan-1-aminium (636 Da, Ligand)
- 5-(dimethylsulfamoyl)-~{n}-(5-methyl-1,3-thiazol-2-yl)-2-pyrrolidin-1-yl-benzamide (394 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit dad1 (12 kDa, Protein from Homo sapiens)
- (2~{s},3~{r},4~{r},5~{s},6~{s})-2-(hydroxymethyl)-6-[(1~{s},2~{r},3~{r},4~{r},5'~{s},6~{s},7~{r},8~{s},9~{r},12~{r},13~{r},15~{s},16~{s},18~{r})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol (610 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3a (80 kDa, Protein from Homo sapiens)
- Oligosaccharyltransferase complex subunit ostc (16 kDa, Protein from Homo sapiens)
- Manganese (ii) ion (54 Da, Ligand)
- (2e,6e,10e,14e,18e,22e,26e)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaenyl trihydrogen diphosphate (722 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (68 kDa, Protein from Homo sapiens)
Cryo-EM structure of yeast Ost6p containing oligosaccharyltransferase complex
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7oci
Q-score: 0.514
Neuhaus JD, Wild R, Eyring J, Irobalieva RN, Kowal J, Lin CW, Locher KP, Aebi M
Glycobiology (2021) 31 pp. 1604-1615 [ Pubmed: 34974622 DOI: doi:10.1093/glycob/cwab084 ]
- Ost6p containing yeast oligosaccharyltransferase complex (Complex from Saccharomyces cerevisiae S288C)
- Dolichylphosphate (847 Da, Ligand)
- Digitonin (1 kDa, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost4 (3 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost5 (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost6 - tm1 (2 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost6 (37 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit wbp1 (49 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit swp1 (31 kDa, Protein from Saccharomyces cerevisiae S288C)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (54 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 (81 kDa, Protein from Saccharomyces cerevisiae S288C)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost2 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM structure of human oligosaccharyltransferase complex OST-A
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6s7o
Q-score: 0.485
Ramirez AS, Kowal J, Locher KP
Science (2019) 366 pp. 1372-1375 [ Pubmed: 31831667 DOI: doi:10.1126/science.aaz3505 ]
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 (4 kDa, Protein from Homo sapiens)
- Transmembrane protein 258 (9 kDa, Protein from Homo sapiens)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (69 kDa, Protein from Homo sapiens)
- Human oligosaccharyltransferase complex (Complex from Homo sapiens)
- (4r,7r)-4-hydroxy-n,n,n-trimethyl-4,9-dioxo-7-[(undecanoyloxy)methyl]-3,5,8-trioxa-4lambda~5~-phosphadocosan-1-aminium (636 Da, Ligand)
- Human oligosaccharyltransferase complex ost-a (Complex)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit dad1 (12 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kda subunit (50 kDa, Protein from Homo sapiens)
- Oligosaccharyltransferase complex subunit ostc (Complex from Homo sapiens)
- [(3~{r},6~{z},10~{z},14~{z},18~{z})-3,7,11,15,19,23-hexamethyltetracosa-6,10,14,18,22-pentaenyl] dihydrogen phosphate (508 Da, Ligand)
- (2~{s},3~{r},4~{r},5~{s},6~{s})-2-(hydroxymethyl)-6-[(1~{s},2~{r},3~{r},4~{r},5'~{s},6~{s},7~{r},8~{s},9~{r},12~{r},13~{r},15~{s},16~{s},18~{r})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol (610 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3a (80 kDa, Protein from Homo sapiens)
- Oligosaccharyltransferase complex subunit ostc (16 kDa, Protein from Homo sapiens)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (68 kDa, Protein from Homo sapiens)
Cryo-EM structure of human oligosaccharyltransferase complex OST-B
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6s7t
Q-score: 0.509
Ramirez AS, Kowal J, Locher KP
Science (2019) 366 pp. 1372-1375 [ Pubmed: 31831667 DOI: doi:10.1126/science.aaz3505 ]
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 (4 kDa, Protein from Homo sapiens)
- Transmembrane protein 258 (9 kDa, Protein from Homo sapiens)
- Human oligosaccharyltransferase complex ost-b (Complex)
- Human oligosaccharyltransferase complex (Complex from Homo sapiens)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (69 kDa, Protein from Homo sapiens)
- Magnesium transporter protein 1 (38 kDa, Protein from Homo sapiens)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3b (93 kDa, Protein from Homo sapiens)
- Magnesium transporter protein 1 (Complex from Homo sapiens)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit dad1 (12 kDa, Protein from Homo sapiens)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kda subunit (50 kDa, Protein from Homo sapiens)
- Peptide (588 Da, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- (2~{s},3~{r},4~{r},5~{s},6~{s})-2-(hydroxymethyl)-6-[(1~{s},2~{r},3~{r},4~{r},5'~{s},6~{s},7~{r},8~{s},9~{r},12~{r},13~{r},15~{s},16~{s},18~{r})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol (610 Da, Ligand)
- Malectin (32 kDa, Protein from Homo sapiens)
- (4r,7r)-4-hydroxy-n,n,n-trimethyl-4,9-dioxo-7-[(undecanoyloxy)methyl]-3,5,8-trioxa-4lambda~5~-phosphadocosan-1-aminium (636 Da, Ligand)
- (2z,6z,10z,14z,18z,22z,26z)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl dihydrogen phosphate (642 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (68 kDa, Protein from Homo sapiens)
Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6ezn
Q-score: 0.494
Wild R, Kowal J, Eyring J, Ngwa EM, Aebi M, Locher KP
Science (2018) 359 pp. 545-550 [ DOI: doi:10.1126/science.aar5140 Pubmed: 29301962 ]
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit wbp1 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit swp1 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Yeast oligosaccharyltransferase (ost) complex (Complex from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (54 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost4 (3 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 (81 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost2 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost5 (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (758 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)