H. sapiens ORC1-5 bound to double stranded DNA as part of the MCM-ORC complex
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8s0c
Q-score: 0.289
Weissmann F, Greiwe JF, Puhringer T, Eastwood EL, Couves EC, Miller TCR, Diffley JFX, Costa A
Nature (2024) 636 pp. 499-508 [ Pubmed: 39604733 DOI: doi:10.1038/s41586-024-08263-6 ]
- Dna (26-mer) (8 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- H. sapiens orc1-5 (Complex from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 1 (97 kDa, Protein from Homo sapiens)
- H. sapiens orc1-5 bound to double stranded dna as part of the mcm-orc complex (400 kDa, Complex)
- Dna (26-mer) (7 kDa, DNA from synthetic construct)
- Double stranded dna (Complex from synthetic construct)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Isoform 2 of origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
H. sapiens MCM bound to double stranded DNA and ORC1-6
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8s0d
Q-score: 0.346
Weissmann F, Greiwe JF, Puhringer T, Eastwood EL, Couves EC, Miller TCR, Diffley JFX, Costa A
Nature (2024) 636 pp. 499-508 [ Pubmed: 39604733 DOI: doi:10.1038/s41586-024-08263-6 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- H. sapiens mcm (Complex from Homo sapiens)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Dna (58-mer) (17 kDa, DNA from synthetic construct)
- H. sapiens orc6 (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Isoform 2 of origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm2 (101 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- Dna (58-mer) (17 kDa, DNA from synthetic construct)
- Origin recognition complex subunit 6 (28 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- H. sapiens orc1-5 (Complex from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 1 (97 kDa, Protein from Homo sapiens)
- Double stranded dna (Complex from synthetic construct)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- H. sapiens mcm bound to double stranded dna and orc1-6 (1 MDa, Complex)
Cryo-EM structure of the 1:1 Orc1 BAH domain in complex with nucleosome
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7e9c
Q-score: 0.311
Jiang H, Yu C, Liu CP, Han X, Yu Z, Xu RM
To Be Published
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Origin recognition complex subunit 1 (Complex)
- Histone h2a.2 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (Complex from Escherichia coli)
- Histone h2b.2 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Histone (Complex from Saccharomyces cerevisiae)
- Yeast orc1 bah domain in complex with 147-bp nucleosome (Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Cryo-EM structure of the 2:1 Orc1 BAH domain in complex with nucleosome
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7e9f
Q-score: 0.319
Jiang H, Yu C, Liu CP, Han X, Yu Z, Xu RM
To Be Published
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Origin recognition complex subunit 1 (Complex)
- Histone h2a.2 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (Complex from Saccharomyces cerevisiae S288C)
- Histone (Complex from Escherichia coli)
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h2b.2 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Yeast orc1 bah domain in complex with 147-bp nucleosome (Complex from Saccharomyces cerevisiae S288C)
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Origin recognition complex subunit 1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Cryo-EM structure of an MCM loading intermediate
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6rqc
Q-score: 0.272
Miller TCR, Locke J, Greiwe JF, Diffley JFX, Costa A
Nature (2019) 575 pp. 704-710 [ Pubmed: 31748745 DOI: doi:10.1038/s41586-019-1768-0 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae S288c)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae S288c)
- The mcm-orc (mo) loading intermediate protein complex (Complex from Saccharomyces cerevisiae S288C)
- Dna (88-mer) (27 kDa, DNA from Saccharomyces cerevisiae S288c)
- The mcm-orc (mo) loading intermediate (Complex)
- Dna (88-mer) (27 kDa, DNA from Saccharomyces cerevisiae S288c)
- Origin recognition complex subunit 1 (108 kDa, Protein from Saccharomyces cerevisiae S288c)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae S288c)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae S288c)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae S288c)
- Orc1-5-orc6c lobe of the mcm-orc (mo) origin licensing intermediate (Complex)
- Mcm-orc6n lobe of the mcm-orc (mo) origin licensing intermediate. (Complex)
- Zinc ion (65 Da, Ligand)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae S288c)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae S288c)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae S288c)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae S288c)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae S288c)
Electron microscopy structure of the Drosophila origin recognition complex
Bleichert F, Balasov M, Chesnokov I, Nogales E, Botchan MR, Berger JM
eLife (2013) 2 pp. e00882-e00882 [ DOI: doi:10.7554/eLife.00882 Pubmed: 24137536 ]
- Orc4 (Protein from Drosophila melanogaster)
- Orc3 (Protein from Drosophila melanogaster)
- Orc5 (Protein from Drosophila melanogaster)
- Drosophila origin recognition complex with atpgs (390 kDa, Sample)
- Orc2 (Protein from Drosophila melanogaster)
- Orc6 (Protein from Drosophila melanogaster)
- Orc1 (Protein from Drosophila melanogaster)
A 3D EM map of the subcomplex Orc1-5 of the yeast origin recognition complex (ORC).
Chen Z, Speck C, Wendel P, Tang C, Stillman B, Li H
PNAS (2008) 105 pp. 10326-10331 [ Pubmed: 18647841 DOI: doi:10.1073/pnas.0803829105 ]
- Orc2 (71 kDa, Protein)
- Chromosomal replication origin recognition protein orc1p (120 kDa, Protein)
- Orc4 (56 kDa, Protein)
- Orc5 (53 kDa, Protein)
- Yeast orc subcomplex orc1-5. (362 kDa, Sample)
- Orc3 (62 kDa, Protein)
ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA.
Speck C, Chen Z, Li H, Stillman B
Nat Struct Mol Biol (2005) 12 pp. 965-971 [ Pubmed: 16228006 DOI: doi:10.1038/nsmb1002 ]
- Orc3p (72 kDa, Protein from Saccharomyces cerevisiae)
- Orc2p (73 kDa, Protein from Saccharomyces cerevisiae)
- Orc4p (61 kDa, Protein from Saccharomyces cerevisiae)
- Orc6p (50 kDa, Protein from Saccharomyces cerevisiae)
- Orc5p (53 kDa, Protein from Saccharomyces cerevisiae)
- Orc1p (120 kDa, Protein from Saccharomyces cerevisiae)
- Yeast origin recognition complex (420 kDa, Sample)
ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA.
Speck C, Chen Z, Li H, Stillman B
Nat Struct Mol Biol (2005) 12 pp. 965-971 [ Pubmed: 16228006 DOI: doi:10.1038/nsmb1002 ]
- Orc3p (72 kDa, Protein from Saccharomyces cerevisiae)
- Orc2p (73 kDa, Protein from Saccharomyces cerevisiae)
- Orc4p (61 kDa, Protein from Saccharomyces cerevisiae)
- Orc6p (50 kDa, Protein from Saccharomyces cerevisiae)
- Orc5p (53 kDa, Protein from Saccharomyces cerevisiae)
- Yeast origin recognition complex and cdc6p (500 kDa, Sample)
- Orc1p (120 kDa, Protein from Saccharomyces cerevisiae)
- Cdc6p (56 kDa, Protein from Saccharomyces cerevisiae)