Structure of the elongating human mitoribosome bound to mtEF-Tu.GMPPCP and A/T mt-tRNA
Resolution: 4.33 Å
EM Method: Single-particle
Fitted PDBs: 7a5g
Q-score: 0.402
Desai N, Yang H, Chandrasekaran V, Kazi R, Minczuk M, Ramakrishnan V
Science (2020) 370 pp. 1105-1110 [ DOI: doi:10.1126/science.abc7782 Pubmed: 33243891 ]
- 39s ribosomal protein l49, mitochondrial (19 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l51, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l2, mitochondrial (33 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l32, mitochondrial (21 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l36, mitochondrial (11 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l40, mitochondrial (24 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l44, mitochondrial (37 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from Homo sapiens)
- E trna (24 kDa, RNA from Homo sapiens)
- Malonate ion (102 Da, Ligand)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l35, mitochondrial (21 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l37, mitochondrial (48 kDa, Protein from Homo sapiens)
- Oxa1l tail (2 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l3, mitochondrial (38 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l17, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l45, mitochondrial (35 kDa, Protein from Homo sapiens)
- 50s ribosomal protein l1 (24 kDa, Protein from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039))
- 12s rrna (306 kDa, RNA from Homo sapiens)
- 39s ribosomal protein s30, mitochondrial,39s ribosomal protein s30, mitochondrial,ml65 (52 kDa, Protein from Homo sapiens)
- Sulfate ion (96 Da, Ligand)
- 39s ribosomal protein l54, mitochondrial (15 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l52, mitochondrial (13 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from Homo sapiens)
- 16s rrna (500 kDa, RNA from Homo sapiens)
- 39s ribosomal protein l24, mitochondrial (24 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Spermidine (145 Da, Ligand)
- 39s ribosomal protein l15, mitochondrial (33 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l42, mitochondrial (16 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l9, mitochondrial (30 kDa, Protein from Homo sapiens)
- Chloride ion (35 Da, Ligand)
- 39s ribosomal protein l47, mitochondrial (29 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l48, mitochondrial (23 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l46, mitochondrial (31 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from Homo sapiens)
- Mrna (3 kDa, RNA from Homo sapiens)
- 39s ribosomal protein l43, mitochondrial (23 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l34, mitochondrial (10 kDa, Protein from Homo sapiens)
- Growth arrest and dna damage-inducible proteins-interacting protein 1 (25 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l27, mitochondrial (16 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l33, mitochondrial (7 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from Homo sapiens)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l14, mitochondrial (15 kDa, Protein from Homo sapiens)
- Ribosomal protein 63, mitochondrial (12 kDa, Protein from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- 39s ribosomal protein l50, mitochondrial (18 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from Homo sapiens)
- Aurora kinase a-interacting protein (22 kDa, Protein from Homo sapiens)
- 39s ribosomal protein s18a, mitochondrial (22 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l39, mitochondrial (38 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 50s ribosomal protein l1 homology model (Complex from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039))
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l53, mitochondrial (12 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from Homo sapiens)
- Human mitoribosome (Complex from Homo sapiens)
- 39s ribosomal protein l20, mitochondrial (17 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l11, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l38, mitochondrial (44 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l13, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l22, mitochondrial (23 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from Homo sapiens)
- Nascent chain (2 kDa, Protein from Homo sapiens)
- P trna (23 kDa, RNA from Homo sapiens)
- 28s ribosomal protein s11, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l19, mitochondrial (33 kDa, Protein from Homo sapiens)
- Elongation factor tu, mitochondrial (49 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from Homo sapiens)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 39s ribosomal protein l41, mitochondrial (15 kDa, Protein from Homo sapiens)
- Peptidyl-trna hydrolase ict1, mitochondrial (23 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from Homo sapiens)
- Mt-trnaval (22 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 39s ribosomal protein l16, mitochondrial (28 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l21, mitochondrial (22 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l23, mitochondrial (17 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l4, mitochondrial (34 kDa, Protein from Homo sapiens)
- Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 (13 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l10, mitochondrial (29 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l18, mitochondrial (20 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*cp*a)-3') (589 Da, RNA from Homo sapiens)
- Human mitoribosome (Complex)
- 39s ribosomal protein l30, mitochondrial (18 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 39s ribosomal protein l28, mitochondrial (30 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l55, mitochondrial (15 kDa, Protein from Homo sapiens)
Structure of the human mitoribosome in the post translocation state bound to mtEF-G1
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7a5k
Q-score: 0.408
Desai N, Yang H, Chandrasekaran V, Kazi R, Minczuk M, Ramakrishnan V
Science (2020) 370 pp. 1105-1110 [ DOI: doi:10.1126/science.abc7782 Pubmed: 33243891 ]
- 39s ribosomal protein l33, mitochondrial (7 kDa, Protein from human)
- 39s ribosomal protein l44, mitochondrial (37 kDa, Protein from human)
- Mitochondrial ribosomal protein l18, isoform cra_b (20 kDa, Protein from human)
- 39s ribosomal protein l41, mitochondrial (15 kDa, Protein from human)
- 39s ribosomal protein l37, mitochondrial (48 kDa, Protein from human)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from human)
- 39s ribosomal protein l47, mitochondrial (29 kDa, Protein from human)
- 39s ribosomal protein l20, mitochondrial (17 kDa, Protein from human)
- 39s ribosomal protein l17, mitochondrial (20 kDa, Protein from human)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from human)
- 39s ribosomal protein l10, mitochondrial (29 kDa, Protein from human)
- Mt-trna (23 kDa, RNA from Homo sapiens)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from human)
- 39s ribosomal protein l4, mitochondrial (34 kDa, Protein from human)
- 39s ribosomal protein l15, mitochondrial (33 kDa, Protein from human)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from human)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from human)
- 39s ribosomal protein l34, mitochondrial (10 kDa, Protein from human)
- 39s ribosomal protein l23, mitochondrial (17 kDa, Protein from human)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from human)
- 12s rrna (306 kDa, RNA from Homo sapiens)
- Ribosomal protein 63, mitochondrial (12 kDa, Protein from human)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from human)
- 39s ribosomal protein l38, mitochondrial (44 kDa, Protein from human)
- 39s ribosomal protein l24, mitochondrial (24 kDa, Protein from human)
- 39s ribosomal protein l16, mitochondrial (28 kDa, Protein from human)
- 39s ribosomal protein l35, mitochondrial (21 kDa, Protein from human)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from human)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from human)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from human)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from human)
- Mt-trnaval (23 kDa, RNA from Homo sapiens)
- 39s ribosomal protein l27, mitochondrial (16 kDa, Protein from human)
- 39s ribosomal protein l55, mitochondrial (15 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- 39s ribosomal protein s18a, mitochondrial (22 kDa, Protein from human)
- Spermidine (145 Da, Ligand)
- 39s ribosomal protein l40, mitochondrial (24 kDa, Protein from human)
- Cdna flj76418, highly similar to homo sapiens mitochondrial ribosomal protein l52 (mrpl52), transcript variant 1, mrna (13 kDa, Protein from human)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from human)
- 39s ribosomal protein l21, mitochondrial (22 kDa, Protein from human)
- 39s ribosomal protein l3, mitochondrial (38 kDa, Protein from human)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from human)
- 39s ribosomal protein l22, mitochondrial (24 kDa, Protein from human)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from human)
- 39s ribosomal protein l46, mitochondrial (31 kDa, Protein from human)
- 39s ribosomal protein l45, mitochondrial (35 kDa, Protein from human)
- 39s ribosomal protein l32, mitochondrial (21 kDa, Protein from human)
- 39s ribosomal protein l36, mitochondrial (11 kDa, Protein from human)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from human)
- 39s ribosomal protein l9, mitochondrial (30 kDa, Protein from human)
- 39s ribosomal protein l48, mitochondrial (21 kDa, Protein from human)
- 39s ribosomal protein l54, mitochondrial (15 kDa, Protein from human)
- 39s ribosomal protein l11, mitochondrial (20 kDa, Protein from human)
- 39s ribosomal protein l19, mitochondrial (33 kDa, Protein from human)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from human)
- 39s ribosomal protein l42, mitochondrial (16 kDa, Protein from human)
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from human)
- Peptidyl-trna hydrolase ict1, mitochondrial (23 kDa, Protein from human)
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from human)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from human)
- Rna (589 Da, RNA from Homo sapiens)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from human)
- Unknown protein (2 kDa, Protein from Homo sapiens)
- Human mitoribosome (Complex from Homo sapiens)
- 39s ribosomal protein l39, mitochondrial (38 kDa, Protein from human)
- Nascent chain (2 kDa, Protein from Homo sapiens)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 39s ribosomal protein l53, mitochondrial (12 kDa, Protein from human)
- 39s ribosomal protein l2, mitochondrial (33 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 (13 kDa, Protein from human)
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from human)
- 39s ribosomal protein l49, mitochondrial (19 kDa, Protein from human)
- 39s ribosomal protein l14, mitochondrial (15 kDa, Protein from human)
- Mitochondrial inner membrane protein oxa1l (48 kDa, Protein from human)
- Growth arrest and dna damage-inducible proteins-interacting protein 1 (25 kDa, Protein from human)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from human)
- 39s ribosomal protein l28, mitochondrial (30 kDa, Protein from human)
- 39s ribosomal protein s30, mitochondrial (50 kDa, Protein from human)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from human)
- Aurora kinase a-interacting protein (22 kDa, Protein from human)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from human)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from human)
- Elongation factor g, mitochondrial (83 kDa, Protein from human)
- 39s ribosomal protein l43, mitochondrial (17 kDa, Protein from human)
- 39s ribosomal protein l1, mitochondrial (32 kDa, Protein from human)
- 39s ribosomal protein l30, mitochondrial (18 kDa, Protein from human)
- 16s rrna (500 kDa, RNA from human)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from human)
- 28s ribosomal protein s11, mitochondrial (20 kDa, Protein from human)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from human)
- 39s ribosomal protein l50, mitochondrial (18 kDa, Protein from human)
- 39s ribosomal protein l13, mitochondrial (20 kDa, Protein from human)
- 39s ribosomal protein l51, mitochondrial (15 kDa, Protein from human)