Cryo-EM structure of the Photosystem I - LHCI supercomplex from Coelastrella sp.
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8cmo
Q-score: 0.537
Fadeeva M, Klaiman D, Nelson N
To Be Published
- Coelastrella sp. photosystem i (900 kDa, Complex from Coelastrella)
- Photosystem i reaction center subunit x psak (8 kDa, Protein from Coelastrella)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- Photosystem i reaction center subunit ii psad (16 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca4 (22 kDa, Protein from Coelastrella)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Photosystem i subunit ix psaj (4 kDa, Protein from Coelastrella)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Light-harvesting protein of photosystem i lhca2 partial (7 kDa, Protein from Coelastrella)
- Photosystem i reaction center subunit iv psae (6 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca3 (23 kDa, Protein from Coelastrella)
- Photosystem i reaction center subunit viii psai (4 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca7 (24 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca8 (22 kDa, Protein from Coelastrella)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Sphingosine (299 Da, Ligand)
- Lauric acid (200 Da, Ligand)
- Light-harvesting protein of photosystem i lhca9 (20 kDa, Protein from Coelastrella)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Chlorophyll b (907 Da, Ligand)
- Water (18 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 psab (81 kDa, Protein from Coelastrella)
- Photosystem i reaction center subunit iii psaf (17 kDa, Protein from Coelastrella)
- Photosystem i p700 chlorophyll a apoprotein a1 psaa (82 kDa, Protein from Coelastrella)
- Light-harvesting protein of photosystem i lhca1 (20 kDa, Protein from Coelastrella)
- Phosphatidylinositol (887 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i reaction center subunit v psag (9 kDa, Protein from Coelastrella)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Octanoic acid (caprylic acid) (144 Da, Ligand)
- Diacyl glycerol (625 Da, Ligand)
- Photosystem i reaction centre subunit xi psal (12 kDa, Protein from Coelastrella)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i subunit vii psac (8 kDa, Protein from Coelastrella)
- Calcium ion (40 Da, Ligand)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Light-harvesting protein of photosystem i lhca5 (24 kDa, Protein from Coelastrella)
- Phylloquinone (450 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Light-harvesting protein of photosystem i lhca6 (24 kDa, Protein from Coelastrella)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Chlamydomonas reinhardtii TSP9 mutant small Photosystem I complex
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 7r3k
Q-score: 0.633
Schwartz T, Fadeeva M, Klaiman D, Nelson N
Biomolecules (2023) 13 [ Pubmed: 36979472 DOI: doi:10.3390/biom13030537 ]
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Chlamydomonas reinhardtii)
- C. reinhardtii photosystem i supercomplex (780 kDa, Complex from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit iv, chloroplastic (10 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit psak, chloroplastic (11 kDa, Protein from Chlamydomonas reinhardtii)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (82 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (28 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (26 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (32 kDa, Protein from Chlamydomonas reinhardtii)
- Ergosterol (396 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- Sphingosine (299 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
- Lauric acid (200 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Chlorophyll b (907 Da, Ligand)
- Water (18 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (27 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (lhca4) (28 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a (893 Da, Ligand)
- Chlorophyll a isomer (893 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Diacyl glycerol (625 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlamydomonas reinhardtii)
- Calcium ion (40 Da, Ligand)
- Photosystem i reaction center subunit v, chloroplastic (13 kDa, Protein from Chlamydomonas reinhardtii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Photosystem i reaction center subunit ii, chloroplastic (21 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit viii (10 kDa, Protein from Chlamydomonas reinhardtii)
- Phylloquinone (450 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit iii, chloroplastic (24 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Photosystem I of a temperature sensitive mutant Chlamydomonas reinhardtii
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 7blx
Q-score: 0.536
Caspy I, Schwartz T, Bayro-Kaiser V, Fadeeva M, Kessel A, Ben-Tal N, Nelson N
Commun Biol (2021) 4 pp. 1380- [ DOI: doi:10.1038/s42003-021-02911-7 ]
- Photosystem i reaction center subunit iv, chloroplastic (7 kDa, Protein from Chlamydomonas reinhardtii)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit ii, chloroplastic (16 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (20 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit iii, chloroplastic (17 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Sphingosine (299 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
- Lauric acid (200 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- (3~{e},5~{e},7~{e})-6-methyl-8-[(6~{r})-2,2,6-trimethylcyclohexyl]octa-3,5,7-trien-2-one (260 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i of temperature sensitive chlamydomonas reinhardtii (890 kDa, Complex from Chlamydomonas reinhardtii)
- Chlorophyll b (907 Da, Ligand)
- Water (18 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit v, chloroplastic (9 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit psak, chloroplastic (8 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a isomer (893 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Octanoic acid (caprylic acid) (144 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- Diacyl glycerol (625 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Chlamydomonas reinhardtii)
- Calcium ion (40 Da, Ligand)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (82 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chlamydomonas reinhardtii)
- Phylloquinone (450 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Psi subunit v (12 kDa, Protein from Chlamydomonas reinhardtii)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Photosystem I of a temperature sensitive mutant Chlamydomonas reinhardtii
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 7bgi
Q-score: 0.629
Caspy I, Schwartz T, Bayro-Kaiser V, Fadeeva M, Kessel A, Ben-Tal N, Nelson N
Commun Biol (2021) 4 pp. 1380- [ DOI: doi:10.1038/s42003-021-02911-7 ]
- Photosystem i reaction center subunit iv, chloroplastic (7 kDa, Protein from Chlamydomonas reinhardtii)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit ii, chloroplastic (16 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (20 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit iii, chloroplastic (17 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Sphingosine (299 Da, Ligand)
- Lauric acid (200 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- (3~{e},5~{e},7~{e})-6-methyl-8-[(6~{r})-2,2,6-trimethylcyclohexyl]octa-3,5,7-trien-2-one (260 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i of temperature sensitive chlamydomonas reinhardtii (890 kDa, Complex from Chlamydomonas reinhardtii)
- Chlorophyll b (907 Da, Ligand)
- Water (18 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit v, chloroplastic (9 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit psak, chloroplastic (8 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (21 kDa, Protein from Chlamydomonas reinhardtii)
- Psi subunit v (14 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a isomer (893 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Octanoic acid (caprylic acid) (144 Da, Ligand)
- Phosphatidylinositol (887 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- Diacyl glycerol (625 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chlamydomonas reinhardtii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlamydomonas reinhardtii)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Chlamydomonas reinhardtii)
- Calcium ion (40 Da, Ligand)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (82 kDa, Protein from Chlamydomonas reinhardtii)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chlamydomonas reinhardtii)
- Phylloquinone (450 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Structure of low-light grown Chlorella ohadii Photosystem I
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6zzx
Q-score: 0.621
Caspy I, Neumann E, Fadeeva M, Liveanu V, Savitsky A, Frank A, Kalisman YL, Shkolnisky Y, Murik O, Treves H, Hartmann V, Nowaczyk MM, Schuhmann W, Rogner M, Willner I, Kaplan A, Schuster G, Nelson N, Lubitz W, Nechushtai R
Nat Plants (2021) 7 pp. 1314-1322 [ Pubmed: 34462576 DOI: doi:10.1038/s41477-021-00983-1 ]
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit chloroplastic (15 kDa, Protein from Chlorella ohadii)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- Photosystem i (1 MDa, Complex from Chlorella ohadii)
- Phylloquinone (450 Da, Ligand)
- Photosystem i subunit o (9 kDa, Protein from Chlorella ohadii)
- Diacyl glycerol (625 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chlorella ohadii)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Astaxanthin (596 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- (1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (8 kDa, Protein from Chlorella ohadii)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (10 kDa, Protein from Chlorella ohadii)
- Lhca4 (23 kDa, Protein from Chlorella ohadii)
- Oleic acid (282 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Glutathione reductase (26 kDa, Protein from Chlorella ohadii)
- Benzene-1,3,5-triol (126 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Beta,beta-caroten-4-one (550 Da, Ligand)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- Water (18 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Psi-f (18 kDa, Protein from Chlorella ohadii)
- Photosystem i p700 chlorophyll a apoprotein a1 (82 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit iv (7 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit vi-chloroplastic-like (10 kDa, Protein from Chlorella ohadii)
- Chlorophyll a (893 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Lhca7 (24 kDa, Protein from Chlorella ohadii)
- Sphingosine (299 Da, Ligand)
- (3~{e},5~{e},7~{e})-6-methyl-8-[(6~{r})-2,2,6-trimethylcyclohexyl]octa-3,5,7-trien-2-one (260 Da, Ligand)
- Photosystem i reaction center subunit xi (16 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- [2-((1-oxododecanoxy-(2-hydroxy-3-propanyl))-phosphonate-oxy)-ethyl]-trimethylammonium (440 Da, Ligand)
- Chlorophyll b (907 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Chlorophyll a isomer (893 Da, Ligand)
- Tripalmitoylglycerol (807 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Chlorella ohadii)
- Palmitic acid (256 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
Structure of high-light grown Chlorella ohadii photosystem I
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 6zzy
Q-score: 0.561
Caspy I, Neumann E, Fadeeva M, Liveanu V, Savitsky A, Frank A, Kalisman YL, Shkolnisky Y, Murik O, Treves H, Hartmann V, Nowaczyk MM, Schuhmann W, Rogner M, Willner I, Kaplan A, Schuster G, Nelson N, Lubitz W, Nechushtai R
Nat Plants (2021) 7 pp. 1314-1322 [ Pubmed: 34462576 DOI: doi:10.1038/s41477-021-00983-1 ]
- Photosystem i reaction center subunit iv (7 kDa, Protein from Chlorella ohadii)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (82 kDa, Protein from Chlorella ohadii)
- Beta,beta-caroten-4-one (550 Da, Ligand)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Tripalmitoylglycerol (807 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Phosphatidylethanolamine (734 Da, Ligand)
- Ergosterol (396 Da, Ligand)
- Psi-f (18 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Sphingosine (299 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (10 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Chlorella ohadii)
- [2-((1-oxododecanoxy-(2-hydroxy-3-propanyl))-phosphonate-oxy)-ethyl]-trimethylammonium (440 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- (3~{e},5~{e},7~{e})-6-methyl-8-[(6~{r})-2,2,6-trimethylcyclohexyl]octa-3,5,7-trien-2-one (260 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (15 kDa, Protein from Chlorella ohadii)
- Chlorophyll b (907 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- 2-(2-azanylethanoylamino)ethanoic acid (132 Da, Ligand)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- Glutathione reductase (26 kDa, Protein from Chlorella ohadii)
- High light grown chlorella ohadii photosystem i (1 MDa, Complex from Chlorella ohadii)
- Chlorophyll a isomer (893 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Chlorella ohadii)
- Palmitic acid (256 Da, Ligand)
- Diacyl glycerol (625 Da, Ligand)
- Oleic acid (282 Da, Ligand)
- Photosystem i reaction center subunit vi-chloroplastic-like (10 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Chlorella ohadii)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chlorella ohadii)
- Psi subunit v (16 kDa, Protein from Chlorella ohadii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chlorella ohadii)
- Phylloquinone (450 Da, Ligand)
- Psi-k (8 kDa, Protein from Chlorella ohadii)
- Beta-carotene (536 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- (2z,4e)-5-[(1s)-1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-1-yl]-3-methylpenta-2,4-dienoic acid (264 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Structure of small high-light grown Chlorella ohadii photosystem I
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7a4p
Q-score: 0.278
Caspy I, Neumann E, Fadeeva M, Liveanu V, Savitsky A, Frank A, Kalisman YL, Shkolnisky Y, Murik O, Treves H, Hartmann V, Nowaczyk MM, Schuhmann W, Rogner M, Willner I, Kaplan A, Schuster G, Nelson N, Lubitz W, Nechushtai R
Nat Plants (2021) 7 pp. 1314-1322 [ Pubmed: 34462576 DOI: doi:10.1038/s41477-021-00983-1 ]
- Photosystem i reaction center subunit iv (7 kDa, Protein from Chlorella ohadii)
- (2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (82 kDa, Protein from Chlorella ohadii)
- Beta,beta-caroten-4-one (550 Da, Ligand)
- Small high light grown chlorella ohadii photosystem i (900 kDa, Complex from Chlorella ohadii)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Tripalmitoylglycerol (807 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- Phosphatidylethanolamine (734 Da, Ligand)
- Ergosterol (396 Da, Ligand)
- Psi-f (18 kDa, Protein from Chlorella ohadii)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Sphingosine (299 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (10 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Chlorella ohadii)
- [2-((1-oxododecanoxy-(2-hydroxy-3-propanyl))-phosphonate-oxy)-ethyl]-trimethylammonium (440 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- (3~{e},5~{e},7~{e})-6-methyl-8-[(6~{r})-2,2,6-trimethylcyclohexyl]octa-3,5,7-trien-2-one (260 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Photosystem i reaction center subunit chloroplastic (15 kDa, Protein from Chlorella ohadii)
- Chlorophyll b (907 Da, Ligand)
- 2-(2-azanylethanoylamino)ethanoic acid (132 Da, Ligand)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (25 kDa, Protein from Chlorella ohadii)
- Glutathione reductase (26 kDa, Protein from Chlorella ohadii)
- Chlorophyll a isomer (893 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- (3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Chlorella ohadii)
- Palmitic acid (256 Da, Ligand)
- Diacyl glycerol (625 Da, Ligand)
- Oleic acid (282 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Chlorella ohadii)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Chlorella ohadii)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Chlorella ohadii)
- Psi subunit v (16 kDa, Protein from Chlorella ohadii)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Chlorella ohadii)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Chlorella ohadii)
- Phylloquinone (450 Da, Ligand)
- Psi-k (8 kDa, Protein from Chlorella ohadii)
- Beta-carotene (536 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (19 kDa, Protein from Chlorella ohadii)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- (1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Photosystem I reduced Plastocyanin Complex
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 6zoo
Q-score: 0.56
Caspy I, Fadeeva M, Kuhlgert S, Borovikova-Sheinker A, Klaiman D, Masrati G, Drepper F, Ben-Tal N, Hippler M, Nelson N
Biochem J (2021) 478 pp. 2371-2384 [ Pubmed: 34085703 DOI: doi:10.1042/BCJ20210267 ]
- Photosystem i p700 chlorophyll a apoprotein a2 (82 kDa, Protein from Garden pea)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Phylloquinone (450 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Garden pea)
- Chlorophyll a-b binding protein 3, chloroplastic (24 kDa, Protein from Garden pea)
- Putative uncharacterized protein (7 kDa, Protein from Garden pea)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (82 kDa, Protein from Garden pea)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (22 kDa, Protein from Garden pea)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Photosystem i reaction center subunit viii (3 kDa, Protein from Garden pea)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Plastocyanin, chloroplastic (10 kDa, Protein from Garden pea)
- Psal domain-containing protein (16 kDa, Protein from Garden pea)
- Chlorophyll a-b binding protein 6, chloroplastic (21 kDa, Protein from Garden pea)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Water (18 Da, Ligand)
- Psad (16 kDa, Protein from Garden pea)
- Photosystem i reaction center subunit vi,photosystem i reaction center subunit vi (10 kDa, Protein from Garden pea)
- Chlorophyll a-b binding protein p4, chloroplastic (21 kDa, Protein from Garden pea)
- Copper (ii) ion (63 Da, Ligand)
- Photosystem i plastocyanin complex (650 kDa, Complex from Pisum sativum)
- Chlorophyll a (893 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit iii (17 kDa, Protein from Garden pea)
- Photosystem i reaction center subunit x psak (8 kDa, Protein from Garden pea)
- Chlorophyll b (907 Da, Ligand)
- Photosystem i reaction center (10 kDa, Protein from Garden pea)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Garden pea)
Dunaliella Minimal Photosystem I
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6yxr
Q-score: 0.453
Caspy I, Malavath T, Klaiman D, Fadeeva M, Shkolnisky Y, Nelson N
Biochim Biophys Acta Bioenerg (2020) 1861 pp. 148253-148253 [ Pubmed: 32569661 DOI: doi:10.1016/j.bbabio.2020.148253 ]
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (21 kDa, Protein from Dunaliella salina)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Psaf (18 kDa, Protein from Dunaliella salina)
- Phylloquinone (450 Da, Ligand)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Dunaliella salina)
- Iron/sulfur cluster (351 Da, Ligand)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Lhca4 (23 kDa, Protein from Dunaliella salina)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Dunaliella salina)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Lhca2 (22 kDa, Protein from Dunaliella salina)
- Chlorophyll a-b binding protein, chloroplastic (22 kDa, Protein from Dunaliella salina)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Large dunaliella salina photosystem i-lhc supercomplex (Complex from Dunaliella salina)
- Chlorophyll a (893 Da, Ligand)
- Psad (15 kDa, Protein from Dunaliella salina)
- Beta-carotene (536 Da, Ligand)
- Chlorophyll b (907 Da, Ligand)
- Psae (7 kDa, Protein from Dunaliella salina)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Dunaliella salina)
- Photosystem i p700 chlorophyll a apoprotein a1 (81 kDa, Protein from Dunaliella salina)
Dunaliella Photosystem I Supercomplex
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 6sl5
Q-score: 0.53
Caspy I, Malavath T, Klaiman D, Fadeeva M, Shkolnisky Y, Nelson N
Biochim Biophys Acta Bioenerg (2020) 1861 pp. 148253-148253 [ Pubmed: 32569661 DOI: doi:10.1016/j.bbabio.2020.148253 ]
- Chlorophyll a-b binding protein, chloroplastic (21 kDa, Protein from Dunaliella salina)
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Psak (8 kDa, Protein from Dunaliella salina)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Tripalmitoylglycerol (807 Da, Ligand)
- [(2~{r})-1-nonanoyloxy-3-[oxidanyl-[(2~{r},3~{s},5~{r},6~{r})-2,3,4,5,6-pentakis(oxidanyl)cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl] (5~{z},8~{z})-heptadeca-5,8-dienoate (722 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Trimethyl-[(2~{r})-1-oxidanyl-1-oxidanylidene-4-[(2~{s})-2-[(1~{s})-1-oxidanyloctadecoxy]-3-[(1~{r})-1-oxidanyloctadecoxy]propoxy]butan-2-yl]azanium (773 Da, Ligand)
- Psae (7 kDa, Protein from Dunaliella salina)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Dunaliella salina)
- Psah (10 kDa, Protein from Dunaliella salina)
- (3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
- Psaf (18 kDa, Protein from Dunaliella salina)
- Chlorophyll b (907 Da, Ligand)
- Psad (16 kDa, Protein from Dunaliella salina)
- Lhca6 (19 kDa, Protein from Dunaliella salina)
- Lhca4 (23 kDa, Protein from Dunaliella salina)
- (3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (81 kDa, Protein from Dunaliella salina)
- Psal (16 kDa, Protein from Dunaliella salina)
- Photosystem i iron-sulfur center (8 kDa, Protein from Dunaliella salina)
- Psai (4 kDa, Protein from Dunaliella salina)
- Octadecanal (268 Da, Ligand)
- Dodecyl-alpha-d-maltoside (510 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (81 kDa, Protein from Dunaliella salina)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a-b binding protein, chloroplastic (24 kDa, Protein from Dunaliella salina)
- Calcium ion (40 Da, Ligand)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Lhca2 (22 kDa, Protein from Dunaliella salina)
- Phylloquinone (450 Da, Ligand)
- Large dunaliella salina photosystem i-lhc supercomplex (Complex from Dunaliella salina)
- Psag (10 kDa, Protein from Dunaliella salina)
- Beta-carotene (536 Da, Ligand)
- Psao (9 kDa, Protein from Dunaliella salina)
- Lhca5 (21 kDa, Protein from Dunaliella salina)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)