Cryo-EM structure of PsBphP in Pr state
Burgie ES, Basore K, Rau MJ, Summers B, Mickles AJ, Grigura V, Fitzpatrick JAJ, Vierstra RD
Nat Commun (2024) 15 pp. 6853-6853 [ Pubmed: 39127720 DOI: doi:10.1038/s41467-024-50412-y ]
- Histidine kinase (82 kDa, Protein from Pseudomonas syringae pv. tomato str. DC3000)
- 3-[2-[(z)-[3-(2-carboxyethyl)-5-[(z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(z)-[(3e)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1h-pyrrol-3- yl]propanoic acid (585 Da, Ligand)
- Psbphp (Complex from Pseudomonas syringae pv. tomato str. DC3000)
Cryo-EM structure of PsBphP in Pfr state, Dimer of Dimers FL
Burgie ES, Basore K, Rau MJ, Summers B, Mickles AJ, Grigura V, Fitzpatrick JAJ, Vierstra RD
Nat Commun (2024) 15 pp. 6853-6853 [ Pubmed: 39127720 DOI: doi:10.1038/s41467-024-50412-y ]
- Histidine kinase (82 kDa, Protein from Pseudomonas syringae pv. tomato str. DC3000)
- 3-[2-[(z)-[3-(2-carboxyethyl)-5-[(z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(z)-[(3e)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1h-pyrrol-3- yl]propanoic acid (585 Da, Ligand)
- Psbphp (Complex from Pseudomonas syringae pv. tomato str. DC3000)
Cryo-EM structure of PsBphP in Pfr state, Dimer of Dimers PSM only
Burgie ES, Basore K, Rau MJ, Summers B, Mickles AJ, Grigura V, Fitzpatrick JAJ, Vierstra RD
Nat Commun (2024) 15 pp. 6853-6853 [ Pubmed: 39127720 DOI: doi:10.1038/s41467-024-50412-y ]
- Histidine kinase (82 kDa, Protein from Pseudomonas syringae pv. tomato str. DC3000)
- 3-[2-[(z)-[3-(2-carboxyethyl)-5-[(z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(z)-[(3e)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1h-pyrrol-3- yl]propanoic acid (585 Da, Ligand)
- Psbphp (Complex from Pseudomonas syringae pv. tomato str. DC3000)
Cryo-EM structure of PsBphP in Pfr state, medial PSM only
Burgie ES, Basore K, Rau MJ, Summers B, Mickles AJ, Grigura V, Fitzpatrick JAJ, Vierstra RD
Nat Commun (2024) 15 pp. 6853-6853 [ Pubmed: 39127720 DOI: doi:10.1038/s41467-024-50412-y ]
- Histidine kinase (82 kDa, Protein from Pseudomonas syringae pv. tomato str. DC3000)
- 3-[2-[(z)-[3-(2-carboxyethyl)-5-[(z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(z)-[(3e)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1h-pyrrol-3- yl]propanoic acid (585 Da, Ligand)
- Psbphp (Complex from Pseudomonas syringae pv. tomato str. DC3000)
Cryo-EM structure of PsBphP in Pfr state, splayed PSM only
Burgie ES, Basore K, Rau MJ, Summers B, Mickles AJ, Grigura V, Fitzpatrick JAJ, Vierstra RD
Nat Commun (2024) 15 pp. 6853-6853 [ Pubmed: 39127720 DOI: doi:10.1038/s41467-024-50412-y ]
- Histidine kinase (82 kDa, Protein from Pseudomonas syringae pv. tomato str. DC3000)
- 3-[2-[(z)-[3-(2-carboxyethyl)-5-[(z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(z)-[(3e)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1h-pyrrol-3- yl]propanoic acid (585 Da, Ligand)
- Psbphp (Complex from Pseudomonas syringae pv. tomato str. DC3000)
Cryo-EM structure of PsBphP in Pr state, extended DHp
Burgie ES, Basore K, Rau MJ, Summers B, Mickles AJ, Grigura V, Fitzpatrick JAJ, Vierstra RD
Nat Commun (2024) 15 pp. 6853-6853 [ Pubmed: 39127720 DOI: doi:10.1038/s41467-024-50412-y ]
- Histidine kinase (82 kDa, Protein from Pseudomonas syringae pv. tomato str. DC3000)
- 3-[2-[(z)-[3-(2-carboxyethyl)-5-[(z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(z)-[(3e)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1h-pyrrol-3- yl]propanoic acid (585 Da, Ligand)
- Psbphp (Complex from Pseudomonas syringae pv. tomato str. DC3000)
Cryo-EM structure of ATP8B1-CDC50A in E2-Pi conformation with occluded PS
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8oxa
Q-score: 0.546
Dieudonne T, Kummerer F, Laursen MJ, Stock C, Flygaard RK, Khalid S, Lenoir G, Lyons JA, Lindorff-Larsen K, Nissen P
Nat Commun (2023) 14 pp. 7492-7492 [ DOI: doi:10.1038/s41467-023-42828-9 Pubmed: 37980352 ]
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Oxido(dioxo)vanadium (98 Da, Ligand)
- Cell cycle control protein 50a (41 kDa, Protein from Homo sapiens)
- (2~{s})-2-azanyl-3-[[(2~{r})-3-hexadecanoyloxy-2-[(~{z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid (762 Da, Ligand)
- Atp8b1-cdc50a complex (185 kDa, Complex from Homo sapiens)
- Phospholipid-transporting atpase ic (136 kDa, Protein from Homo sapiens)
SARS-CoV-2 spike glycoprotein trimer in closed state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7wgv
Q-score: 0.513
Zhao MM, Zhu Y, Zhang L, Zhong G, Tai L, Liu S, Yin G, Lu J, He Q, Li MJ, Zhao RX, Wang H, Huang W, Fan C, Shuai L, Wen Z, Wang C, He X, Chen Q, Liu B, Xiong X, Bu Z, Wang Y, Sun F, Yang JK
Cell Discov (2022) 8 pp. 53- [ DOI: doi:10.1038/s41421-022-00419-w ]
- 3-[5-[(z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-2-[[5-[(z)-(3-ethenyl-4-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1h-pyrrol-2-yl]methyl]-4-methyl-1h-pyrrol-3-yl]propanoic acid (584 Da, Ligand)
- Spike glycoprotein (133 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 b.1.1.7 spike glycoprotein trimer (Complex from Severe acute respiratory syndrome coronavirus 2)
- Linoleic acid (280 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
SARS-CoV-2 spike glycoprotein trimer in closed state after treatment with Cathepsin L
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7wgx
Q-score: 0.468
Zhao MM, Zhu Y, Zhang L, Zhong G, Tai L, Liu S, Yin G, Lu J, He Q, Li MJ, Zhao RX, Wang H, Huang W, Fan C, Shuai L, Wen Z, Wang C, He X, Chen Q, Liu B, Xiong X, Bu Z, Wang Y, Sun F, Yang JK
Cell Discov (2022) 8 pp. 53- [ DOI: doi:10.1038/s41421-022-00419-w ]
- 3-[5-[(z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-2-[[5-[(z)-(3-ethenyl-4-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1h-pyrrol-2-yl]methyl]-4-methyl-1h-pyrrol-3-yl]propanoic acid (584 Da, Ligand)
- Spike glycoprotein (133 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sars-cov-2 b.1.1.7 spike glycoprotein trimer (Complex from Severe acute respiratory syndrome coronavirus 2)