Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 9cgp
Q-score: 0.408
Iyer KA, Kobayashi T, Murayama T, Samso M
Structure (2025) 33 pp. 338-348.e4 [ DOI: doi:10.1016/j.str.2024.11.018 Pubmed: 39708816 ]
- Ryanodine receptor 1 (565 kDa, Protein from Oryctolagus cuniculus)
- Ryr1 disease mutant y523s with fkbp12.6, nanodisc and inhibitor dantrolene in the absence of calcium with refined p1 domain (2 MDa, Complex from Oryctolagus cuniculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dantrolene (314 Da, Ligand)
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 9cgq
Q-score: 0.463
Iyer KA, Kobayashi T, Murayama T, Samso M
To Be Published
- Ryanodine receptor 1 (565 kDa, Protein from Oryctolagus cuniculus)
- Ryr1 disease mutant y523s with fkbp12.6, nanodisc and inhibitor dantrolene in the presence of activating calcium with refined p1 domain (2 MDa, Complex from Oryctolagus cuniculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Dantrolene (314 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8dty
Q-score: 0.309
Hu Y, Iyer KA, Nayak AR, Eltit JM, Kurebayashi N, Murayama T, Samso M
To Be Published
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Recombinant mouse ryr2 triple phosphomimetic mutant s2807d/s2813d/s2030d in complex with fkbp12.6 and nanodisc under closed-state conditions (2 MDa, Complex from Mus musculus)
- Ryanodine receptor 2 (565 kDa, Protein from Mus musculus)
- Zinc ion (65 Da, Ligand)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8dtz
Q-score: 0.302
Hu Y, Iyer KA, Nayak AR, Eltit JM, Kurebayashi N, Murayama T, Samso M
To Be Published
- Ryanodine receptor 2 (565 kDa, Protein from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Recombinant mouse ryr2 triple phosphonull mutant s2807a/s2813a/s2030a in complex with fkbp12.6 and nanodisc under closed-state conditions (2 MDa, Complex from Mus musculus)
Resolution: 3.68 Å
EM Method: Single-particle
Fitted PDBs: 8dvv
Q-score: 0.318
Hu Y, Iyer KA, Nayak AR, Eltit JM, Kurebayashi N, Murayama T, Samso M
To Be Published
- Recombinant mouse ryr2 triple phosphomimetic mutant s2807d/s2813d/s2030d in complex with fkbp12.6 and nanodisc under open-state conditions (2 MDa, Complex from Mus musculus)
- Ryanodine receptor 2 (565 kDa, Protein from Mus musculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7t64
Q-score: 0.306
Iyer KA, Hu Y, Klose T, Murayama T, Samso M
PNAS (2022) 119 pp. e2122140119-e2122140119 [ Pubmed: 35867837 DOI: doi:10.1073/pnas.2122140119 ]
- Zinc ion (65 Da, Ligand)
- Rabbit ryr1 disease mutant y523s in complex with fkbp12.6 embedded in lipidic nanodisc in the closed state (2 MDa, Complex)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Ryanodine receptor 1 (565 kDa, Protein from Oryctolagus cuniculus)
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 7t65
Q-score: 0.348
Iyer KA, Hu Y, Klose T, Murayama T, Samso M
PNAS (2022) 119 pp. e2122140119-e2122140119 [ DOI: doi:10.1073/pnas.2122140119 Pubmed: 35867837 ]
- Ryanodine receptor 1 (565 kDa, Protein from Oryctolagus cuniculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Rabbit ryr1 disease mutant y523s in complex with fkbp12.6 embedded in lipidic nanodisc in the open state (2 MDa, Complex)
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 6wot
Q-score: 0.32
Iyer KA, Hu Y, Nayak AR, Kurebayashi N, Murayama T, Samso M
Sci Adv (2020) 6 pp. eabb2964-eabb2964 [ Pubmed: 32832689 DOI: doi:10.1126/sciadv.abb2964 ]
- Recombinant rabbit ryanodine receptor type 1, ryr1, with mutation r164c (Complex from Oryctolagus cuniculus)
- Recombinant rabbit ryanodine receptor type 1, ryr1, with mutation r164c in complex with fkbp12.6 (2 MDa, Complex)
- Fkbp12.6 (Complex from Homo sapiens)
- Ryanodine receptor 1 (565 kDa, Protein from Oryctolagus cuniculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 6wou
Q-score: 0.376
Iyer KA, Hu Y, Nayak AR, Kurebayashi N, Murayama T, Samso M
Sci Adv (2020) 6 pp. eabb2964-eabb2964 [ Pubmed: 32832689 DOI: doi:10.1126/sciadv.abb2964 ]
- Ryanodine receptor 2 (565 kDa, Protein from Mus musculus)
- Fkbp12.6 (Complex from Homo sapiens)
- Recombinant mouse ryanodine receptor type 2, ryr2, with mutation r176q in complex with fkbp12.6 (2 MDa, Complex)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Recombinant mouse ryanodine receptor type 2, ryr2, with mutation r176q (Complex from Mus musculus)
Cryo-EM structure of recombinant mouse Ryanodine Receptor type 2 wild type in complex with FKBP12.6
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 6wov
Q-score: 0.133
Iyer KA, Hu Y, Nayak AR, Kurebayashi N, Murayama T, Samso M
Sci Adv (2020) 6 pp. eabb2964-eabb2964 [ Pubmed: 32832689 DOI: doi:10.1126/sciadv.abb2964 ]
- Recombinant mouse ryanodine receptor type 2, ryr2, wild type in complex with fkbp12.6 (2 MDa, Complex)
- Ryanodine receptor 2 (565 kDa, Protein from Mus musculus)
- Recombinant mouse ryanodine receptor type 2, ryr2, wild type (Complex from Mus musculus)
- Fkbp12.6 (Complex from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)