Cryo-EM structure of recombinant wildtype ACTA1 phalloidin-stabilized F-actin
Resolution: 3.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 9duu
Q-score: 0.531
Garg A, Jansen S, Greenberg L, Zhang R, Lavine KJ, Greenberg MJ
PNAS (2024) 121 pp. e2405020121-e2405020121 [ DOI: doi:10.1073/pnas.2405020121 Pubmed: 39503885 ]
- Acta1 f-actin (Complex from Homo sapiens)
- Phalloidin (Complex from Amanita phalloides)
- Actin, alpha skeletal muscle (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of recombinant wildtype acta1 phalloidin-stabilized f-actin (Complex)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of recombinant R254H ACTA1 phalloidin-stabilized F-actin
Resolution: 3.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 9duv
Q-score: 0.528
Garg A, Jansen S, Greenberg L, Zhang R, Lavine KJ, Greenberg MJ
PNAS (2024) 121 pp. e2405020121-e2405020121 [ DOI: doi:10.1073/pnas.2405020121 Pubmed: 39503885 ]
- R256h acta1 f-actin (Complex from Homo sapiens)
- Actin, alpha skeletal muscle (41 kDa, Protein from Homo sapiens)
- Phalloidin (Complex from Amanita phalloides)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cryo-em structure of recombinant r256h acta1 phalloidin-stabilized f-actin (Complex)
Cryo-EM structure of the phalloidin-bound pointed end of the actin filament.
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 9fjm
Q-score: 0.476
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ DOI: doi:10.1038/s41467-024-52251-3 Pubmed: 39261469 ]
- Magnesium ion (24 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Actin filament pointed end (Complex from Homo sapiens)
- Actin filament pointed end bound by phalloidin (Complex)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 1)
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 9fju
Q-score: 0.399
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ DOI: doi:10.1038/s41467-024-52251-3 Pubmed: 39261469 ]
- Magnesium ion (24 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin filament pointed end (Complex from Bos taurus)
- Phosphate ion (94 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Two dnase i molecules, each bound to the ultimate and penultimate actin subunits of the f-actin pointed end. (Complex from Bos taurus)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Bos taurus)
- Deoxyribonuclease-1 (31 kDa, Protein from Bos taurus)
- F-actin pointed end bound by dnase i and phalloidin. (Complex)
Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 2).
Resolution: 3.79 Å
EM Method: Single-particle
Fitted PDBs: 9fjy
Q-score: 0.412
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ DOI: doi:10.1038/s41467-024-52251-3 Pubmed: 39261469 ]
- Magnesium ion (24 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin filament pointed end (Complex from Bos taurus)
- Phosphate ion (94 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Two dnase i molecules, each bound to the ultimate and penultimate actin subunits of the f-actin pointed end. (Complex from Bos taurus)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Bos taurus)
- Deoxyribonuclease-1 (31 kDa, Protein from Bos taurus)
- F-actin pointed end bound by dnase i and phalloidin. (Complex)
Smooth Muscle Gamma Actin (ACTG2) Filament Mutant R40C
Resolution: 2.54 Å
EM Method: Helical reconstruction
Fitted PDBs: 8v30
Q-score: 0.585
Ceron RH, Baez-Cruz FA, Palmer NJ, Carman PJ, Boczkowska M, Heuckeroth RO, Ostap EM, Dominguez R
Sci Adv (2024) 10 pp. eadn6615-eadn6615 [ DOI: doi:10.1126/sciadv.adn6615 Pubmed: 38820162 ]
- Magnesium ion (24 Da, Ligand)
- Actg2 r40c filament (Complex from Homo sapiens)
- Phalloidin (808 Da, Protein from synthetic construct)
- Actin, gamma-enteric smooth muscle (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Phalloidin. (Complex from Amanita phalloides)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Actin filament (Complex from Homo sapiens)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.17
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Magnesium ion (24 Da, Ligand)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Phalloidin (Complex from Amanita phalloides)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
- Phosphate ion (94 Da, Ligand)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Actin filament (Complex from Homo sapiens)
Cryo-EM structure of cortactin stabilized Arp2/3-complex nucleated actin branches
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8p94
Q-score: 0.452
Liu T, Cao L, Mladenov M, Jegou A, Way M, Moores CA
Nat Struct Mol Biol (2024) 31 pp. 801-809 [ Pubmed: 38267598 DOI: doi:10.1038/s41594-023-01205-2 ]
- Phalloidin (Complex from Amanita phalloides)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mouse capping protein (Complex from Mus musculus)
- Actin-related protein 2/3 complex subunit 5-like protein (16 kDa, Protein from Homo sapiens)
- Isoform 2 of f-actin-capping protein subunit beta (30 kDa, Protein from Mus musculus)
- Cortactin stabilizes arp2/3-complex nucleated actin branches with daughter filament capped (Complex from Homo sapiens)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Actin-related protein 3 (47 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Mouse cortactin (Complex from Mus musculus)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from Homo sapiens)
- Actin-related protein 2/3 complex subunit 1b (41 kDa, Protein from Homo sapiens)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from Homo sapiens)
- Actin-related protein 2 (44 kDa, Protein from Homo sapiens)
- Porcine actin, cytoplasmic 1 (Complex from Sus scrofa)
- F-actin-capping protein subunit alpha-1 (32 kDa, Protein from Mus musculus)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Homo sapiens)
- Human arp2/3 c1bc5l complex (Complex from Homo sapiens)
- Src substrate cortactin (61 kDa, Protein from Mus musculus)
Cryo-EM structure of the undecorated barbed end of filamentous beta/gamma actin
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 8oi6
Q-score: 0.464
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ DOI: doi:10.1038/s41594-023-01101-9 Pubmed: 37749275 ]
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cytosolic beta-gamma actin (Complex from Bos taurus)
- Phalloidin (Complex from Amanita phalloides)
- Actin-phalloidin complex (Complex)