Cryo-EM structure of the phalloidin-bound pointed end of the actin filament.
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 9fjm
Q-score: 0.476
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ Pubmed: 39261469 DOI: doi:10.1038/s41467-024-52251-3 ]
- Actin filament pointed end bound by phalloidin (Complex)
- Phosphate ion (94 Da, Ligand)
- Actin filament pointed end (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Phalloidin (Complex from Amanita phalloides)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 1)
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 9fju
Q-score: 0.399
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ Pubmed: 39261469 DOI: doi:10.1038/s41467-024-52251-3 ]
- Actin filament pointed end (Complex from Bos taurus)
- Phosphate ion (94 Da, Ligand)
- Deoxyribonuclease-1 (31 kDa, Protein from Bos taurus)
- F-actin pointed end bound by dnase i and phalloidin. (Complex)
- Two dnase i molecules, each bound to the ultimate and penultimate actin subunits of the f-actin pointed end. (Complex from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 2).
Resolution: 3.79 Å
EM Method: Single-particle
Fitted PDBs: 9fjy
Q-score: 0.412
Boiero Sanders M, Oosterheert W, Hofnagel O, Bieling P, Raunser S
Nat Commun (2024) 15 pp. 7969-7969 [ Pubmed: 39261469 DOI: doi:10.1038/s41467-024-52251-3 ]
- Actin filament pointed end (Complex from Bos taurus)
- Phosphate ion (94 Da, Ligand)
- Deoxyribonuclease-1 (31 kDa, Protein from Bos taurus)
- F-actin pointed end bound by dnase i and phalloidin. (Complex)
- Two dnase i molecules, each bound to the ultimate and penultimate actin subunits of the f-actin pointed end. (Complex from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Actin filament (Complex from Homo sapiens)
- Phosphate ion (94 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Phalloidin. (Complex from Amanita phalloides)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.17
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Actin filament (Complex from Homo sapiens)
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Phosphate ion (94 Da, Ligand)
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the undecorated barbed end of filamentous beta/gamma actin
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 8oi6
Q-score: 0.464
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ DOI: doi:10.1038/s41594-023-01101-9 Pubmed: 37749275 ]
- Cytosolic beta-gamma actin (Complex from Bos taurus)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Phalloidin (Complex from Amanita phalloides)
- Actin-phalloidin complex (Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Bos taurus)
Structure of capping protein bound to the barbed end of a cytoplasmic actin filament
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7pdz
Q-score: 0.333
Funk J, Merino F, Schaks M, Rottner K, Raunser S, Bieling P
Nat Commun (2021) 12 pp. 5329-5329 [ DOI: doi:10.1038/s41467-021-25682-5 Pubmed: 34504078 ]
- Isoform 2 of f-actin-capping protein subunit beta (30 kDa, Protein from Mus musculus)
- Phosphate ion (94 Da, Ligand)
- Phalloidin (808 Da, Protein from synthetic construct)
- Complex between capping protein and the barbed end of cytoplasmic actin filaments (Complex)
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Bovine)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- F-actin-capping protein subunit alpha-1 (32 kDa, Protein from Mus musculus)