Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Phalloidin. (Complex from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Actin filament (Complex from Homo sapiens)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
Structure of the P. aeruginosa ExoY-F-actin complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7p1g
Q-score: 0.46
Belyy A, Merino F, Mechold U, Raunser S
Nat Commun (2021) 12 pp. 6628-6628 [ Pubmed: 34785651 DOI: doi:10.1038/s41467-021-26889-2 ]
- Magnesium ion (24 Da, Ligand)
- Maltose/maltodextrin-binding periplasmic protein,adenylate cyclase exoy (84 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Phosphate ion (94 Da, Ligand)
- The complex of p. aeruginosa exoy with f-actin (Complex)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- 3'-deoxy-guanosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Structure of Dip1-activated Arp2/3 complex with nucleated actin filament
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6w17
Q-score: 0.409
Shaaban M, Chowdhury S, Nolen BJ
Nat Struct Mol Biol (2020) 27 pp. 1009-1016 [ Pubmed: 32839613 DOI: doi:10.1038/s41594-020-0481-x ]
- Magnesium ion (24 Da, Ligand)
- Complex consisting of actin-filament nucleator, arp2/3 complex associated with nucleation promoting factor dip1 and first four actin subunits from the pointed end of the nucleated actin-filament (446 kDa, Complex from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from Fission yeast)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Fission yeast)
- Protein dip1 (43 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 3 (47 kDa, Protein from Fission yeast)
- Phalloidin (808 Da, Protein from death cap)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Fission yeast)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Fission yeast)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from Fission yeast)
- Actin-related protein 2 (44 kDa, Protein from Fission yeast)
Phalloidin bound F-actin complex
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7bti
Q-score: 0.519
Kumari A, Kesarwani S, Javoor MG, Vinothkumar KR, Sirajuddin M
EMBO J (2020) 39 pp. e104006-e104006 [ DOI: doi:10.15252/embj.2019104006 Pubmed: 32567727 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Filamentous actin in adp state (Complex from Gallus gallus)
- Actin, alpha skeletal muscle (42 kDa, Protein from Chicken)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Cryo-EM structure of FLNaABD E254K bound to phalloidin-stabilized F-actin
Resolution: 3.54 Å
EM Method: Helical reconstruction
Fitted PDBs: 6d8c
Q-score: 0.452
Iwamoto DV, Huehn A, Simon B, Huet-Calderwood C, Baldassarre M, Sindelar CV, Calderwood DA
Nat Struct Mol Biol (2018) 25 pp. 918-927 [ DOI: doi:10.1038/s41594-018-0128-3 Pubmed: 30224736 ]
- Magnesium ion (24 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Gallus gallus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Helical complex of flnaabd e254k bound to phalloidin-stabilized f-actin (Complex from Homo sapiens)
- Filamin-a (34 kDa, Protein from Homo sapiens)
- Phalloidin (808 Da, Protein from Amanita phalloides)