Structure of the Lifeact-F-actin complex
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 7ad9
Q-score: 0.466
Belyy A, Merino F, Sitsel O, Raunser S
PLoS Biol (2020) 18 pp. e3000925-e3000925 [ Pubmed: 33216759 DOI: doi:10.1371/journal.pbio.3000925 ]
- Phalloidin (Complex from synthetic construct)
- Phosphate ion (94 Da, Ligand)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Actin (Complex from Oryctolagus cuniculus)
- Phalloidin (808 Da, Protein from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Complex between skeletal actin from rabbit, phalloidin and lifeact (163 MDa, Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lifeact (Complex from Saccharomyces cerevisiae)
- Lifeact (1 kDa, Protein from Saccharomyces cerevisiae)
Structure of Dip1-activated Arp2/3 complex with nucleated actin filament
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6w17
Q-score: 0.409
Shaaban M, Chowdhury S, Nolen BJ
Nat Struct Mol Biol (2020) 27 pp. 1009-1016 [ DOI: doi:10.1038/s41594-020-0481-x Pubmed: 32839613 ]
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from Fission yeast)
- Actin-related protein 2 (44 kDa, Protein from Fission yeast)
- Complex consisting of actin-filament nucleator, arp2/3 complex associated with nucleation promoting factor dip1 and first four actin subunits from the pointed end of the nucleated actin-filament (446 kDa, Complex from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from Fission yeast)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Fission yeast)
- Actin-related protein 3 (47 kDa, Protein from Fission yeast)
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Phalloidin (808 Da, Protein from death cap)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Fission yeast)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Protein dip1 (43 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Fission yeast)
Phalloidin bound F-actin complex
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7bti
Q-score: 0.519
Kumari A, Kesarwani S, Javoor MG, Vinothkumar KR, Sirajuddin M
EMBO J (2020) 39 pp. e104006-e104006 [ Pubmed: 32567727 DOI: doi:10.15252/embj.2019104006 ]
- Filamentous actin in adp state (Complex from Gallus gallus)
- Actin, alpha skeletal muscle (42 kDa, Protein from Chicken)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
Actin phalloidin at BeFx state
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 6u96
Q-score: 0.497
Das S, Ge P, Oztug Durer ZA, Grintsevich EE, Zhou ZH, Reisler E
Structure (2020) 28 pp. 586- [ DOI: doi:10.1016/j.str.2020.04.004 Pubmed: 32348747 ]
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Phalloidin derivative (808 Da, Protein from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin phalloidin, befx state (Cellular component from Oryctolagus cuniculus)
Cryo-EM structure of phalloidin-stabilized F-actin (copolymerized)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6t1y
Q-score: 0.506
Pospich S, Merino F, Raunser S
Structure (2020) 28 pp. 437-449.e5 [ Pubmed: 32084355 DOI: doi:10.1016/j.str.2020.01.014 ]
- Phalloidin (808 Da, Protein from synthetic construct)
- Phalloidin (Complex from synthetic construct)
- Filamentous alpha actin in complex with adp-pi (Complex from Oryctolagus cuniculus)
- Actin, alpha skeletal muscle (41 kDa, Protein from Rabbit)
- Filamentous phalloidin-stabilized alpha actin in complex with adp-pi (Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of phalloidin-stabilized F-actin (aged)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6t20
Q-score: 0.446
Pospich S, Merino F, Raunser S
Structure (2020) 28 pp. 437-449.e5 [ Pubmed: 32084355 DOI: doi:10.1016/j.str.2020.01.014 ]
- Phalloidin (808 Da, Protein from synthetic construct)
- Phalloidin (Complex from synthetic construct)
- Actin, alpha skeletal muscle (41 kDa, Protein from Rabbit)
- Magnesium ion (24 Da, Ligand)
- Filamentous alpha actin in complex with adp (Complex from Oryctolagus cuniculus)
- Filamentous phalloidin-stabilized alpha actin in complex with adp (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of phalloidin-Alexa Flour-546-stabilized F-actin (copolymerized)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6t25
Q-score: 0.464
Pospich S, Merino F, Raunser S
Structure (2020) 28 pp. 437-449.e5 [ Pubmed: 32084355 DOI: doi:10.1016/j.str.2020.01.014 ]
- Phalloidin (808 Da, Protein from synthetic construct)
- Phalloidin (Complex from synthetic construct)
- Filamentous alpha actin in complex with adp-pi (Complex from Oryctolagus cuniculus)
- Actin, alpha skeletal muscle (41 kDa, Protein from Rabbit)
- Filamentous phalloidin-stabilized alpha actin in complex with adp-pi (Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)