Cryo-EM structure of E.coli MlaFEB with AMPPNP
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ch6
Q-score: 0.506
Zhou C, Shi H, Zhang M, Zhou L, Xiao L, Feng S, Im W, Zhou M, Zhang X, Huang Y
J Mol Biol (2021) 433 pp. 166986-166986 [ DOI: doi:10.1016/j.jmb.2021.166986 Pubmed: 33845086 ]
- E.coli mlafeb bond with amppnp (Complex from Escherichia coli K12)
- Lipid asymmetry maintenance protein mlab (10 kDa, Protein from Escherichia coli (strain K12))
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Phospholipid abc transporter atp-binding protein mlaf (29 kDa, Protein from Escherichia coli (strain K12))
- Lipid asymmetry maintenance abc transporter permease subunit mlae (27 kDa, Protein from Escherichia coli (strain K12))
Cryo-EM structure of E.coli MlaFEB
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7ch7
Q-score: 0.415
Zhou C, Shi H, Zhang M, Zhou L, Xiao L, Feng S, Im W, Zhou M, Zhang X, Huang Y
J Mol Biol (2021) 433 pp. 166986-166986 [ Pubmed: 33845086 DOI: doi:10.1016/j.jmb.2021.166986 ]
- Lipid asymmetry maintenance protein mlab (10 kDa, Protein from Escherichia coli (strain K12))
- Lipid asymmetry maintenance abc transporter permease subunit mlae (27 kDa, Protein from Escherichia coli (strain K12))
- Phospholipid abc transporter atp-binding protein mlaf (29 kDa, Protein from Escherichia coli (strain K12))
- E.coli mla feb complex (Complex from Escherichia coli K-12)
Cryo_EM map of nucleotide free MlaFEDB complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7cge
Q-score: 0.559
Chi X, Fan Q, Zhang Y, Liang K, Wan L, Zhou Q, Li Y
Cell Res (2020) 30 pp. 1127-1135 [ Pubmed: 32884137 DOI: doi:10.1038/s41422-020-00404-6 ]
- Cryo_em map of nucleotide free mlafedb complex (Complex from Escherichia coli K-12)
- Outer membrane lipid asymmetry maintenance protein mlad (19 kDa, Protein from Escherichia coli (strain K12))
- Lipid asymmetry maintenance protein mlab (10 kDa, Protein from Escherichia coli (strain K12))
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Phospholipid abc transporter atp-binding protein mlaf (29 kDa, Protein from Escherichia coli (strain K12))
- Lipid asymmetry maintenance abc transporter permease subunit mlae (27 kDa, Protein from Escherichia coli (strain K12))
Cryo EM map of the MlaFEDB complex in ATP-bound EQtall conformation (Mutation of E170Q on MlaF)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7cgn
Q-score: 0.308
Chi X, Fan Q, Zhang Y, Liang K, Wan L, Zhou Q, Li Y
Cell Res (2020) 30 pp. 1127-1135 [ Pubmed: 32884137 DOI: doi:10.1038/s41422-020-00404-6 ]
- Cryo em map of the mlafedb complex in atp-bound eqtall conformation (mutation of e170q on mlaf) (Complex from Escherichia coli K-12)
- Phospholipid abc transporter atp-binding protein mlaf (29 kDa, Protein from Escherichia coli (strain K12))
- Outer membrane lipid asymmetry maintenance protein mlad (19 kDa, Protein from Escherichia coli (strain K12))
- Lipid asymmetry maintenance protein mlab (10 kDa, Protein from Escherichia coli (strain K12))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Lipid asymmetry maintenance abc transporter permease subunit mlae (27 kDa, Protein from Escherichia coli (strain K12))
Cryo EM map of the MlaFEDB complex in ATP-bound EQclose conformation (Mutation of E170Q on MlaF)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7ch0
Q-score: 0.487
Chi X, Fan Q, Zhang Y, Liang K, Wan L, Zhou Q, Li Y
Cell Res (2020) 30 pp. 1127-1135 [ Pubmed: 32884137 DOI: doi:10.1038/s41422-020-00404-6 ]
- Lipid asymmetry maintenance abc transporter permease subunit mlae (27 kDa, Protein from Escherichia coli K-12)
- Cryo em map of the mlafedb complex in atp-bound eqclose conformation (mutation of e170q on mlaf) (Complex from Escherichia coli K-12)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Lipid asymmetry maintenance protein mlab (10 kDa, Protein from Escherichia coli K-12)
- Outer membrane lipid asymmetry maintenance protein mlad (19 kDa, Protein from Escherichia coli K-12)
- Phospholipid abc transporter atp-binding protein mlaf (29 kDa, Protein from Escherichia coli K-12)