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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

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offer vital services to scientists in the member states

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scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"Pre-mRNA-splicing factor CWC26" AND resolution:[3 TO 4}

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EMD-6972

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Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part)

Release date: Aug. 29, 2018
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5zwm
Q-score: 0.162

Bai R, Wan R, Yan C, Lei J, Shi Y

Science (2018) 360 pp. 1423-1429 [ DOI: doi:10.1126/science.aau0325 Pubmed: 29794219 ]

Sample:

U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae S288c)
Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae S288c)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288c)
66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrnp component ist3 (17 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-bps (13 kDa, RNA from Saccharomyces cerevisiae S288c)
U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
Magnesium ion (24 Da, Ligand)
Pre-mrna leakage protein 1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
Yeast fully assembled spliceosomal pre-b complex before activation (Cell from Saccharomyces cerevisiae)
Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae S288c)
Zinc ion (65 Da, Ligand)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor cwc26 (30 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)

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EMD-6974

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Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom

Release date: Aug. 29, 2018
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5zwo
Q-score: 0.191

Bai R, Wan R, Yan C, Lei J, Shi Y

Science (2018) 360 pp. 1423-1429 [ DOI: doi:10.1126/science.aau0325 Pubmed: 29794219 ]

Sample:

U6 snrna-associated sm-like protein lsm2 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-processing factor 31 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor prp9 (63 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm4 (21 kDa, Protein from Saccharomyces cerevisiae S288c)
Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm5 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor spp381 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
23 kda u4/u6.u5 small nuclear ribonucleoprotein component (22 kDa, Protein from Saccharomyces cerevisiae S288c)
U4 snrna (51 kDa, RNA from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae S288c)
Yeast fully assembled spliceosomal b complex before activation (Cell from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm7 (13 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae S288c)
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Saccharomyces cerevisiae S288c)
66 kda u4/u6.u5 small nuclear ribonucleoprotein component (66 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
U2 snrnp component ist3 (17 kDa, Protein from Saccharomyces cerevisiae S288c)
U4/u6 small nuclear ribonucleoprotein prp3 (55 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm8 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
U4/u6 small nuclear ribonucleoprotein prp4 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
Magnesium ion (24 Da, Ligand)
Pre-mrna leakage protein 1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 8 (279 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna (19 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna-associated sm-like protein lsm3 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae S288c)
Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor cwc26 (30 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 6 (104 kDa, Protein from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 38 (27 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae S288c)
U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae S288c)
Spliceosomal protein dib1 (16 kDa, Protein from Saccharomyces cerevisiae S288c)

[Show more]

EMD-9524

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Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution

Release date: Sept. 21, 2016
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 5gm6
Q-score: 0.377

Yan C, Wan R, Bai R, Huang G, Shi Y

Science (2016) 353 pp. 904-911 [ DOI: doi:10.1126/science.aag0291 Pubmed: 27445306 ]

Sample:

Pre-mrna-splicing factor syf1 (78 kDa, Protein from Saccharomyces cerevisiae S288c)
U2 snrnp component hsh155 (110 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
U2 snrnp component ist3 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor clf1 (68 kDa, Protein from Saccharomyces cerevisiae S288c)
Small nuclear ribonucleoprotein sm d2 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Adenosine-5'-diphosphate (427 Da, Ligand)
Pre-mrna (18 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna leakage protein 1 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rds3 complex subunit 10 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Guanosine-5'-triphosphate (523 Da, Ligand)
Pre-mrna-splicing factor prp11 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor cef1 (61 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor prp46 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor rds3 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Protein hsh49 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor cwc26 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Small nuclear ribonucleoprotein f (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor bud31 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Saccharomyces cerevisiae strain cdrdr_sf_h chromosome vii sequence (68 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor cwc24 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Magnesium ion (24 Da, Ligand)
Cold sensitive u2 snrna suppressor 1 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor snu114 (114 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Saccharomyces cerevisiae)
Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna (7 kDa, RNA from Saccharomyces cerevisiae S288c)
Zinc ion (65 Da, Ligand)
U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor syf2 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor snt309 (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor atp-dependent rna helicase-like protein prp2 (99 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Saccharomyces cerevisiae strain t.52_2h chromosome xii sequence (35 kDa, RNA from Saccharomyces cerevisiae)
Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Peptidyl-prolyl isomerase cwc27 (35 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-splicing factor rse1 (153 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-mrna-processing factor 17 (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Bact spliceosomal complex (2 MDa, Complex from Saccharomyces cerevisiae S288c)
Pre-mrna-splicing factor 8 (265 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMDB [3]

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