Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8hfr
Q-score: 0.574
Li Z, Chen S, Zhao L, Huang G, Xu H, Yang X, Wang P, Gao N, Sui SF
Nature (2023) 618 pp. 411-418 [ DOI: doi:10.1038/s41586-023-06128-y Pubmed: 37258668 ]
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nucleolar gtp-binding protein 2 (55 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- Mrna export factor mex67 (67 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal export protein nmd3 (59 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae S288C)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Shuttling pre-60s factor ecm1 (23 kDa, Protein from Saccharomyces cerevisiae S288C)
- Yeast 60s ribosomal protein l1-a (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- Yeast 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- Mrna transport regulator mtr2 (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Translation machinery-associated protein 16 (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Npc-trapped pre-60s ribosome (Complex from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae S288C)
Nucleoplasmic pre-60S intermediate of the Nog2 containing pre-rotation state
Resolution: 2.34 Å
EM Method: Single-particle
Fitted PDBs: 7uoo
Q-score: 0.633
Sekulski K, Cruz VE, Weirich CS, Erzberger JP
Nat Commun (2023) 14 pp. 1207-1207 [ DOI: doi:10.1038/s41467-023-36867-5 Pubmed: 36864048 ]
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 2 (55 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Potassium ion (39 Da, Ligand)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome production factor 2 homolog (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Water (18 Da, Ligand)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-propane-1,3-diol (282 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nop53 (52 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis regulatory protein (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rsa4 isoform 1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Its2 rrna (27 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on tif6 and nog2. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (35 kDa, Protein from Saccharomyces cerevisiae BY4741)
Resolution: 2.43 Å
EM Method: Single-particle
Fitted PDBs: 7uqb
Q-score: 0.621
Sekulski K, Cruz VE, Weirich CS, Erzberger JP
Nat Commun (2023) 14 pp. 1207-1207 [ DOI: doi:10.1038/s41467-023-36867-5 Pubmed: 36864048 ]
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rsa4 isoform 1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 2 (55 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Potassium ion (39 Da, Ligand)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome production factor 2 homolog (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Water (18 Da, Ligand)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-propane-1,3-diol (282 Da, Ligand)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Its2 rrna (23 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nop53 (52 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on tif6 and nog2 from a spb1-d52a strain. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nuclear gtp-binding protein nug1 (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
Nucleoplasmic pre-60S intermediate of the Nog2 containing pre-rotation state from a SPB1 D52A strain
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7uqz
Q-score: 0.608
Sekulski K, Cruz VE, Weirich CS, Erzberger JP
Nat Commun (2023) 14 pp. 1207-1207 [ Pubmed: 36864048 DOI: doi:10.1038/s41467-023-36867-5 ]
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 2 (53 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome production factor 2 homolog (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Bud site selection protein 20 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Potassium ion (39 Da, Ligand)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- Water (18 Da, Ligand)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Its2 rrna (23 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (38 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on tif6 and nog2 from a spb1 d52a strain (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nop53 (52 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-propane-1,3-diol (282 Da, Ligand)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rsa4 isoform 1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 7v08
Q-score: 0.623
Sekulski K, Cruz VE, Weirich CS, Erzberger JP
Nat Commun (2023) 14 pp. 1207-1207 [ Pubmed: 36864048 DOI: doi:10.1038/s41467-023-36867-5 ]
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome production factor 2 homolog (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rlp7 isoform 1 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on tif6 and nog2. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Potassium ion (39 Da, Ligand)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rpl8a isoform 1 (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Water (18 Da, Ligand)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rpl38 isoform 1 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nop53 (52 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis regulatory protein (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-propane-1,3-diol (282 Da, Ligand)
- Its2 (27 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rsa4 isoform 1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 2 (55 kDa, Protein from Saccharomyces cerevisiae BY4741)
Structure of pre-60S particle bound to DRG1(AFG2)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7z34
Q-score: 0.227
Prattes M, Grishkovskaya I, Hodirnau VV, Hetzmannseder C, Zisser G, Sailer C, Kargas V, Loibl M, Gerhalter M, Kofler L, Warren AJ, Stengel F, Haselbach D, Bergler H
Nat Struct Mol Biol (2022) 29 pp. 942-953 [ Pubmed: 36097293 DOI: doi:10.1038/s41594-022-00832-5 ]
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Unknown protein (14 kDa, Protein from Saccharomyces cerevisiae S288C ATCC 204508 / S288c)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Atpase family gene 2 protein (84 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal export protein nmd3 (59 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae S288C)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 35s pre-ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae S288C)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- Unknown peptide (1 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-60s ribosomal particle in complex with drg1(afg2) (Complex from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae S288C)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6m62
Q-score: 0.489
Micic J, Li Y, Wu S, Wilson D, Tutuncuoglu B, Gao N, Woolford Jr JL
Nat Commun (2020) 11 pp. 3751-3751 [ DOI: doi:10.1038/s41467-020-17534-5 Pubmed: 32719344 ]
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein nop53 (52 kDa, Protein from Saccharomyces cerevisiae S288c)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- Eukaryotic pre-60s ribosomal subunits (Complex from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Nucleolar gtp-binding protein 2 (55 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- Its2-1 miscrna (74 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome assembly protein 4 (57 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae S288c)
- Zinc ion (65 Da, Ligand)
- Ribosome biogenesis protein rpf2 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae S288c)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288c)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- Rdn5-2 rrna (38 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae S288c)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Rrna-processing protein cgr1 (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae S288c)
- Regulator of ribosome biosynthesis (23 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288c)
- Rdn25-1 rrna (1 MDa, RNA from Saccharomyces cerevisiae S288C)
- Rdn58-1 rrna (50 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae S288c)
Rix1-Rea1 pre-60S particle - 60S core, body 1 (rigid body refinement)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6ylg
Q-score: 0.545
Kater L, Mitterer V, Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E
Mol Cell (2020) 79 pp. 615-628.e5 [ DOI: doi:10.1016/j.molcel.2020.06.032 Pubmed: 32668200 ]
- 60s ribosomal protein l1-a (24 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
- 5s rrna (38 kDa, RNA from Baker's yeast)
- 60s ribosomal protein l7-a (27 kDa, Protein from Baker's yeast)
- 5.8s rrna (50 kDa, RNA from Baker's yeast)
- 60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l8-a (28 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l22-a (13 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
- Rix1-rea1 pre-60s assembly particle - 60s core portion (Complex from Saccharomyces cerevisiae)
- Nucleolar gtp-binding protein 2 (55 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l25 (15 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
- Upf0642 protein ybl028c (12 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l11-a (19 kDa, Protein from Baker's yeast)
- 25s rrna (1 MDa, RNA from Baker's yeast)
- 60s ribosomal protein l14-a (15 kDa, Protein from Baker's yeast)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein ipi1 (37 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l43-a (10 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rrna-processing protein cgr1 (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l30 (11 kDa, Protein from Baker's yeast)
- Bud site selection protein 20 (18 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l13-a (22 kDa, Protein from Baker's yeast)
- Protein sda1 (86 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l12-a (17 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l5 (33 kDa, Protein from Baker's yeast)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Baker's yeast)
- Probable metalloprotease arx1 (65 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l34-a (13 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l6-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
- Ribosome assembly protein 4 (57 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Baker's yeast)
Rix1-Rea1 pre-60S particle - full composite structure
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6ylh
Q-score: 0.512
Kater L, Mitterer V, Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E
Mol Cell (2020) 79 pp. 615-628.e5 [ DOI: doi:10.1016/j.molcel.2020.06.032 Pubmed: 32668200 ]
- 60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein ipi1 (37 kDa, Protein from Baker's yeast)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Baker's yeast)
- 5s rrna (38 kDa, RNA from Baker's yeast)
- 60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l5 (33 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l11-a (19 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l34-a (13 kDa, Protein from Baker's yeast)
- Rrna-processing protein cgr1 (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l22-a (13 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l12-a (17 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
- Ribosome assembly protein 4 (57 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
- Probable metalloprotease arx1 (65 kDa, Protein from Baker's yeast)
- Rix1-rea1 pre-60s assembly particle - composite structure of the entire particle (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l30 (11 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
- Nucleolar gtp-binding protein 2 (55 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
- Upf0642 protein ybl028c (12 kDa, Protein from Baker's yeast)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Baker's yeast)
- Bud site selection protein 20 (18 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l43-a (10 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein rix1 (86 kDa, Protein from Baker's yeast)
- 5.8s rrna (50 kDa, RNA from Baker's yeast)
- 60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l25 (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l1-a (24 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l8-a (28 kDa, Protein from Baker's yeast)
- Pre-rrna-processing protein ipi3 (61 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
- Midasin (559 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l13-a (22 kDa, Protein from Baker's yeast)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
- Protein sda1 (86 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l6-a (20 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l7-a (27 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l14-a (15 kDa, Protein from Baker's yeast)
- 25s rrna (1 MDa, RNA from Baker's yeast)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Baker's yeast)
Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rzz
Q-score: 0.53
Kargas V, Castro-Hartmann P, Escudero-Urquijo N, Dent K, Hilcenko C, Sailer C, Zisser G, Marques-Carvalho MJ, Pellegrino S, Wawiorka L, Freund SM, Wagstaff JL, Andreeva A, Faille A, Chen E, Stengel F, Bergler H, Warren AJ
eLife (2019) 8 [ DOI: doi:10.7554/eLife.44904 Pubmed: 31115337 ]
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Ul1 (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-b (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae)
- Large subunit gtpase 1 (72 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal export protein nmd3 (59 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l24-a (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- Cytoplasmic 60s subunit biogenesis factor rei1 (45 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Lsg1-tap 60s ribosomal subunit (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae)
- Tyrosine-protein phosphatase yvh1 (41 kDa, Protein from Saccharomyces cerevisiae)