Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8ow1
Q-score: 0.165
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ DOI: doi:10.1126/sciadv.adg7480 Pubmed: 37506202 ]
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the CBF3-core-Ndc10-DBD complex of the budding yeast kinetochore
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6gyp
Q-score: 0.402
Yan K, Zhang Z, Yang J, McLaughlin SH, Barford D
Nat Struct Mol Biol (2018) 25 pp. 1103-1110 [ Pubmed: 30478265 DOI: doi:10.1038/s41594-018-0154-1 ]
- Cbf3-core-ndc10-dbd complex (Complex from Saccharomyces cerevisiae S288C)
- Threonine (119 Da, Ligand)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae S288C)
- Arginine (175 Da, Ligand)
- Centromere dna-binding protein complex cbf3 subunit a (112 kDa, Protein from Saccharomyces cerevisiae S288C)
- Methionine (149 Da, Ligand)
- Phenylalanine (165 Da, Ligand)
- Glutamine (146 Da, Ligand)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae S288C)
- Asparagine (132 Da, Ligand)
- Centromere dna-binding protein complex cbf3 subunit b (66 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6gys
Q-score: 0.245
Yan K, Zhang Z, Yang J, McLaughlin SH, Barford D
Nat Struct Mol Biol (2018) 25 pp. 1103-1110 [ Pubmed: 30478265 DOI: doi:10.1038/s41594-018-0154-1 ]
- Dna (52-mer) (16 kDa, DNA from Saccharomyces cerevisiae)
- Cryo-em structure of the cbf3-cen3 complex of the budding yeast kinetochore (Complex from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit a (112 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae)
- Dna (52-mer) (15 kDa, DNA from Saccharomyces cerevisiae)
Cryo-EM structure of the CBF3-msk complex of the budding yeast kinetochore
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6gyu
Q-score: 0.47
Yan K, Zhang Z, Yang J, McLaughlin SH, Barford D
Nat Struct Mol Biol (2018) 25 pp. 1103-1110 [ Pubmed: 30478265 DOI: doi:10.1038/s41594-018-0154-1 ]
- Zinc ion (65 Da, Ligand)
- Centromere dna-binding protein complex cbf3 subunit a (112 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Centromere dna-binding protein complex cbf3 subunit b (66 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cbf3-msk complex (Complex from Saccharomyces cerevisiae S288C)
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))