Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs
Resolution: 3.39 Å
EM Method: Single-particle
Fitted PDBs: 7ado
Q-score: 0.458
Miller-Vedam LE, Brauning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Stroud RM, Craik CS, Schulman BA, Frost A, Weissman JS
eLife (2020) 9 [ Pubmed: 33236988 DOI: doi:10.7554/eLife.62611 ]
- Er membrane protein complex subunit 4 (20 kDa, Protein from Homo sapiens)
- Unassigned helix (1 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 1 (111 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 3 (29 kDa, Protein from Homo sapiens)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Er membrane protein complex subunit 6 (12 kDa, Protein from Homo sapiens)
- Human endoplasmic reticulum membrane protein complex (emc) in popc nanodiscs (300 kDa, Complex from Homo sapiens)
- Membrane magnesium transporter 1 (15 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 10 (27 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 8 (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Er membrane protein complex subunit 2 (34 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 7 (26 kDa, Protein from Homo sapiens)
Cryo-EM structure of human ER membrane protein complex in GDN detergent
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7adp
Q-score: 0.43
Miller-Vedam LE, Brauning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Stroud RM, Craik CS, Schulman BA, Frost A, Weissman JS
eLife (2020) 9 [ Pubmed: 33236988 DOI: doi:10.7554/eLife.62611 ]
- Er membrane protein complex subunit 4 (20 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 1 (111 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 3 (29 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 6 (12 kDa, Protein from Homo sapiens)
- Human endoplasmic reticulum membrane protein complex (emc) in popc nanodiscs (300 kDa, Complex from Homo sapiens)
- Membrane magnesium transporter 1 (15 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 10 (27 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 8 (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Er membrane protein complex subunit 2 (34 kDa, Protein from Homo sapiens)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7ktx
Q-score: 0.287
Miller-Vedam LE, Brauning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Stroud RM, Craik CS, Schulman BA, Frost A, Weissman JS
eLife (2020) 9 [ Pubmed: 33236988 DOI: doi:10.7554/eLife.62611 ]
- Fab dh4 light chain (25 kDa, Protein from Homo sapiens)
- Fab dh4 (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Protein sop4 (26 kDa, Protein from Saccharomyces cerevisiae BY4743)
- Er membrane protein complex subunit 6 (12 kDa, Protein from Saccharomyces cerevisiae BY4743)
- Er membrane protein complex subunit 2 (33 kDa, Protein from Saccharomyces cerevisiae BY4743)
- Unassigned helix (2 kDa, Protein from Saccharomyces cerevisiae BY4743)
- Er membrane protein complex subunit 1 (87 kDa, Protein from Saccharomyces cerevisiae BY4743)
- Endoplasmic reticulum membrane protein complex subunit 10 (22 kDa, Protein from Saccharomyces cerevisiae BY4743)
- Er membrane protein complex subunit 3 (28 kDa, Protein from Saccharomyces cerevisiae BY4743)
- Er membrane protein complex subunit 4 (21 kDa, Protein from Saccharomyces cerevisiae BY4743)
- Fab dh4 heavy chain (26 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 5 (20 kDa, Protein from Saccharomyces cerevisiae BY4743)
- S. cerevisiae endoplasmic reticulum membrane protein complex (emc) bound to fab in ddm detergent (Complex)
- Endoplasmic reticulum membrane protein complex (Complex from Saccharomyces cerevisiae BY4743)
Human ER membrane protein complex in a lipid nanodisc, multi-body refinement, lumenal map
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6ww7
Q-score: 0.35
Pleiner T, Tomaleri GP, Januszyk K, Inglis AJ, Hazu M, Voorhees RM
Science (2020) 369 pp. 433-436 [ DOI: doi:10.1126/science.abb5008 Pubmed: 32439656 ]
Human ER membrane protein complex in a lipid nanodisc, multi-body refinement, cytosolic map
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6ww7
Q-score: 0.199
Pleiner T, Tomaleri GP, Januszyk K, Inglis AJ, Hazu M, Voorhees RM
Science (2020) 369 pp. 433-436 [ DOI: doi:10.1126/science.abb5008 Pubmed: 32439656 ]
Human ER membrane protein complex in a lipid nanodisc, overall map
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6ww7
Q-score: 0.49
Pleiner T, Tomaleri GP, Januszyk K, Inglis AJ, Hazu M, Voorhees RM
Science (2020) 369 pp. 433-436 [ DOI: doi:10.1126/science.abb5008 Pubmed: 32439656 ]
- Membrane magnesium transporter 1 (14 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 10 (27 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 1 (111 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 3 (29 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 6 (12 kDa, Protein from Homo sapiens)
- Human er membrane protein complex (Complex from Homo sapiens)
- Er membrane protein complex subunit 8 (23 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 4 (1 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Er membrane protein complex subunit 2 (34 kDa, Protein from Homo sapiens)
- Er membrane protein complex subunit 7 (26 kDa, Protein from Homo sapiens)