Structure of McrBC (stalkless mutant)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7vsr
Q-score: 0.214
Saikrishnan K, Adhav VA, Bose S, Kutti R V
To Be Published
- 5-methylcytosine-specific restriction enzyme b (54 kDa, Protein from Escherichia coli (strain K12))
- Protein mcrc (36 kDa, Protein from Escherichia coli (strain K12))
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Mcrbc (stalkless mutant) (720 kDa, Complex from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the Escherichia coli McrBC complex
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 6ut6
Q-score: 0.477
Niu Y, Suzuki H, Hosford CJ, Walz T, Chappie JS
Nat Commun (2020) 11 pp. 5907-5907 [ DOI: doi:10.1038/s41467-020-19735-4 Pubmed: 33219217 ]
- Protein mcrc (40 kDa, Protein from Escherichia coli (strain K12))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 5-methylcytosine-specific restriction enzyme b (53 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Complex of the full-length e. coli mcrbc (Cellular component from Escherichia coli (strain K12))
- 5'-guanosine-diphosphate-monothiophosphate (539 Da, Ligand)
Structure of McrBC without DNA binding domains (one half of the full complex)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6hz4
Q-score: 0.456
Nirwan N, Itoh Y, Singh P, Bandyopadhyay S, Vinothkumar KR, Amunts A, Saikrishnan K
Nat Commun (2019) 10 pp. 3058-3058 [ DOI: doi:10.1038/s41467-019-11084-1 Pubmed: 31296862 ]
- Mcrb and mcrc complex without dna binding domains (510 kDa, Complex from Escherichia coli K-12)
- Protein mcrc (40 kDa, Protein from Escherichia coli (strain K12))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 5-methylcytosine-specific restriction enzyme b (35 kDa, Protein from Escherichia coli (strain K12))
Structure of McrBC without DNA binding domains (Class 1)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6hz5
Q-score: 0.34
Nirwan N, Itoh Y, Singh P, Bandyopadhyay S, Vinothkumar KR, Amunts A, Saikrishnan K
Nat Commun (2019) 10 pp. 3058-3058 [ DOI: doi:10.1038/s41467-019-11084-1 Pubmed: 31296862 ]
- Mcrb and mcrc complex without dna binding domains (510 kDa, Complex from Escherichia coli K-12)
- Protein mcrc (40 kDa, Protein from Escherichia coli (strain K12))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 5-methylcytosine-specific restriction enzyme b (35 kDa, Protein from Escherichia coli (strain K12))
Structure of McrBC without DNA binding domains (Class 2)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6hz6
Q-score: 0.329
Nirwan N, Itoh Y, Singh P, Bandyopadhyay S, Vinothkumar KR, Amunts A, Saikrishnan K
Nat Commun (2019) 10 pp. 3058-3058 [ DOI: doi:10.1038/s41467-019-11084-1 Pubmed: 31296862 ]
- Mcrb and mcrc complex without dna binding domains (510 kDa, Complex from Escherichia coli K-12)
- Protein mcrc (40 kDa, Protein from Escherichia coli (strain K12))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 5-methylcytosine-specific restriction enzyme b (35 kDa, Protein from Escherichia coli (strain K12))
Structure of McrBC without DNA binding domains (Class 3)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6hz7
Q-score: 0.314
Nirwan N, Itoh Y, Singh P, Bandyopadhyay S, Vinothkumar KR, Amunts A, Saikrishnan K
Nat Commun (2019) 10 pp. 3058-3058 [ DOI: doi:10.1038/s41467-019-11084-1 Pubmed: 31296862 ]
- Mcrb and mcrc complex without dna binding domains (510 kDa, Complex from Escherichia coli K-12)
- Protein mcrc (40 kDa, Protein from Escherichia coli (strain K12))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 5-methylcytosine-specific restriction enzyme b (35 kDa, Protein from Escherichia coli (strain K12))
Structure of McrBC without DNA binding domains (Class 4)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6hz8
Q-score: 0.299
Nirwan N, Itoh Y, Singh P, Bandyopadhyay S, Vinothkumar KR, Amunts A, Saikrishnan K
Nat Commun (2019) 10 pp. 3058-3058 [ DOI: doi:10.1038/s41467-019-11084-1 Pubmed: 31296862 ]
- Mcrb and mcrc complex without dna binding domains (510 kDa, Complex from Escherichia coli K-12)
- Protein mcrc (40 kDa, Protein from Escherichia coli (strain K12))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 5-methylcytosine-specific restriction enzyme b (35 kDa, Protein from Escherichia coli (strain K12))
Structure of McrBC without DNA binding domains (Class 5)
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 6hz9
Q-score: 0.251
Nirwan N, Itoh Y, Singh P, Bandyopadhyay S, Vinothkumar KR, Amunts A, Saikrishnan K
Nat Commun (2019) 10 pp. 3058-3058 [ DOI: doi:10.1038/s41467-019-11084-1 Pubmed: 31296862 ]
- Mcrb and mcrc complex without dna binding domains (510 kDa, Complex from Escherichia coli K-12)
- Protein mcrc (40 kDa, Protein from Escherichia coli (strain K12))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 5-methylcytosine-specific restriction enzyme b (35 kDa, Protein from Escherichia coli (strain K12))