Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7tfh
Q-score: 0.469
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Magnesium ion (24 Da, Ligand)
- Template strand (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Rfc-pcna-dna1-dna2 (Complex)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dsdna (Complex from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Proteins (Complex from Saccharomyces cerevisiae)
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 7tfi
Q-score: 0.444
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Magnesium ion (24 Da, Ligand)
- Template strand (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Rfc-pcna-dna1-dna2 (Complex)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dsdna (Complex from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Proteins (Complex from Saccharomyces cerevisiae)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7tfj
Q-score: 0.458
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Magnesium ion (24 Da, Ligand)
- Rfc-pcna-dna1 (Complex)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (12 kDa, DNA from synthetic construct)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dsdna (Complex from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Proteins (Complex from Saccharomyces cerevisiae)
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7tfk
Q-score: 0.39
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Magnesium ion (24 Da, Ligand)
- Template strand (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Rfc-pcna-dna1-dna2 (Complex)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dsdna (Complex from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Proteins (Complex from Saccharomyces cerevisiae)
Cryo-EM 3D map of S. cerevisiae clamp loader RFC bound to DNA
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 7tfl
Q-score: 0.329
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Magnesium ion (24 Da, Ligand)
- Template strand (12 kDa, DNA from synthetic construct)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Rfc-pcna-dna1-dna2 (Complex)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dsdna (Complex from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Proteins (Complex from Saccharomyces cerevisiae)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7kc0
Q-score: 0.442
Zheng F, Georgescu RE, Li H, O'Donnell ME
PNAS (2020) 117 pp. 30344-30353 [ Pubmed: 33203675 DOI: doi:10.1073/pnas.2017637117 ]
- Dna polymerase (124 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dsdna (Complex from Saccharomyces cerevisiae)
- Pol32 isoform 1 (40 kDa, Protein from Saccharomyces cerevisiae)
- Dna (5'-d(p*ap*tp*gp*ap*cp*cp*ap*tp*gp*ap*tp*tp*ap*cp*gp*ap*ap*tp*tp*gp*c)-3') (7 kDa, DNA from Saccharomyces cerevisiae)
- Pol delta-pcna-dna (Complex)
- Iron/sulfur cluster (351 Da, Ligand)
- 2',3'-dideoxy-thymidine-5'-triphosphate (466 Da, Ligand)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Pol31 isoform 1 (55 kDa, Protein from Saccharomyces cerevisiae)
- Proteins (Complex from Saccharomyces cerevisiae)
- Dna (25-mer) (11 kDa, DNA from Saccharomyces cerevisiae)