Cryo-EM structure of "CT-CT dimer" of Lactococcus lactis pyruvate carboxylase with acetyl-CoA
Resolution: 2.15 Å
EM Method: Single-particle
Fitted PDBs: 7zz6
Q-score: 0.676
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Dodu A, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Pyruvic acid (88 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
- Magnesium ion (24 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
Cryo-EM structure of Lactococcus lactis pyruvate carboxylase with acetyl-CoA
Resolution: 2.12 Å
EM Method: Single-particle
Fitted PDBs: 7zyy
Q-score: 0.64
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Bicarbonate ion (61 Da, Ligand)
- Pyruvic acid (88 Da, Ligand)
- Acetyl coenzyme *a (809 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
- Magnesium ion (24 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.47 Å
EM Method: Single-particle
Fitted PDBs: 7zyz
Q-score: 0.642
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Oxaloacetate ion (131 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 7zz0
Q-score: 0.646
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
- Magnesium ion (24 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.27 Å
EM Method: Single-particle
Fitted PDBs: 7zz1
Q-score: 0.617
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Biotin (244 Da, Ligand)
- Oxaloacetate ion (131 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
- Magnesium ion (24 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.48 Å
EM Method: Single-particle
Fitted PDBs: 7zz2
Q-score: 0.602
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Pyruvic acid (88 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
- Magnesium ion (24 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 7zz3
Q-score: 0.591
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Bicarbonate ion (61 Da, Ligand)
- Pyruvic acid (88 Da, Ligand)
- Biotin (244 Da, Ligand)
- Acetyl coenzyme *a (809 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.63 Å
EM Method: Single-particle
Fitted PDBs: 7zz4
Q-score: 0.55
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Acetyl coenzyme *a (809 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.43 Å
EM Method: Single-particle
Fitted PDBs: 7zz5
Q-score: 0.572
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Bicarbonate ion (61 Da, Ligand)
- Acetyl coenzyme *a (809 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a (Cellular component from Lactococcus lactis)
Cryo-EM structure of Lactococcus lactis pyruvate carboxylase with acetyl-CoA and cyclic di-AMP
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7zz8
Q-score: 0.521
Lopez-Alonso JP, Lazaro M, Gil-Carton D, Choi PH, Tong L, Valle M
Nat Commun (2022) 13 pp. 6185-6185 [ Pubmed: 36261450 DOI: doi:10.1038/s41467-022-33987-2 ]
- Pyruvate carboxylase (127 kDa, Protein from Lactococcus lactis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Pyruvic acid (88 Da, Ligand)
- (2r,3r,3as,5r,7ar,9r,10r,10as,12r,14ar)-2,9-bis(6-amino-9h-purin-9-yl)octahydro-2h,7h-difuro[3,2-d:3',2'-j][1,3,7,9,2,8 ]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide (658 Da, Ligand)
- Acetyl coenzyme *a (809 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Pyruvate carboxylase with acetyl coenzyme a and cyclic di-amp (Cellular component from Lactococcus lactis)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)