Cryo-EM structure of the mouse TRPM8 channel in complex with PI(4,5)P2 and Ca2+
Yin Y, Park CG, Zhang F, G Fedor J, Feng S, Suo Y, Im W, Lee SY
Sci Adv (2024) 10 pp. eadp2211-eadp2211 [ DOI: doi:10.1126/sciadv.adp2211 Pubmed: 39093967 ]
- Transient receptor potential cation channel subfamily m member 8 (Complex from Mus musculus)
- Calcium ion (40 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 8 (131 kDa, Protein from Mus musculus)
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 8tz4
Q-score: 0.503
The open state mouse TRPM8 structure in complex with the cooling agonist C3, AITC, and PI(4,5)P2
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8e4l
Q-score: 0.407
Yin Y, Zhang F, Feng S, Butay KJ, Borgnia MJ, Im W, Lee SY
Science (2022) 378 pp. eadd1268-eadd1268 [ DOI: doi:10.1126/science.add1268 Pubmed: 36227998 ]
- Transient receptor potential cation channel subfamily m member 8 (131 kDa, Protein from Mus musculus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 8 (Complex from Mus musculus)
- Nonyl(oxo)di(propan-2-yl)-lambda~5~-phosphane (260 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 3-isothiocyanatoprop-1-ene (99 Da, Ligand)
The intermediate C2-state mouse TRPM8 structure in complex with the cooling agonist C3 and PI(4,5)P2
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8e4m
Q-score: 0.384
Yin Y, Zhang F, Feng S, Butay KJ, Borgnia MJ, Im W, Lee SY
Science (2022) 378 pp. eadd1268-eadd1268 [ DOI: doi:10.1126/science.add1268 Pubmed: 36227998 ]
- Transient receptor potential cation channel subfamily m member 8 (131 kDa, Protein from Mus musculus)
- Transient receptor potential cation channel subfamily m member 8 (Complex from Mus musculus)
- Nonyl(oxo)di(propan-2-yl)-lambda~5~-phosphane (260 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
The closed C1-state mouse TRPM8 structure in complex with PI(4,5)P2
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8e4n
Q-score: 0.436
Yin Y, Zhang F, Feng S, Butay KJ, Borgnia MJ, Im W, Lee SY
Science (2022) 378 pp. eadd1268-eadd1268 [ DOI: doi:10.1126/science.add1268 Pubmed: 36227998 ]
- Transient receptor potential cation channel subfamily m member 8 (Complex from Mus musculus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 8 (131 kDa, Protein from Mus musculus)
The closed C1-state mouse TRPM8 structure in complex with putative PI(4,5)P2
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8e4o
Q-score: 0.425
Yin Y, Zhang F, Feng S, Butay KJ, Borgnia MJ, Im W, Lee SY
Science (2022) 378 pp. eadd1268-eadd1268 [ DOI: doi:10.1126/science.add1268 Pubmed: 36227998 ]
- Transient receptor potential cation channel subfamily m member 8 (Complex from Mus musculus)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 8 (131 kDa, Protein from Mus musculus)
The closed C0-state flycatcher TRPM8 structure in complex with PI(4,5)P2
Resolution: 3.51 Å
EM Method: Single-particle
Fitted PDBs: 8e4q
Q-score: 0.451
Yin Y, Zhang F, Feng S, Butay KJ, Borgnia MJ, Im W, Lee SY
Science (2022) 378 pp. eadd1268-eadd1268 [ DOI: doi:10.1126/science.add1268 Pubmed: 36227998 ]
- Transient receptor potential cation channel subfamily m member 8 (131 kDa, Protein from Ficedula albicollis)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 8 (Complex from Ficedula albicollis)