human KCNQ1-CaM-ML277-PIP2 complex in state A
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7xnl
Q-score: 0.412
Ma D, Zhong L, Yan Z, Yao J, Zhang Y, Ye F, Huang Y, Lai D, Yang W, Hou P, Guo J
PNAS (2022) 119 pp. e2207067119-e2207067119 [ Pubmed: 36763058 DOI: doi:10.1073/pnas.2207067119 ]
- (2r)-n-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-1-(4-methylbenzene-1-sulfonyl)piperidine-2-carboxamide (471 Da, Ligand)
- Calmodulin-3 (19 kDa, Protein from Homo sapiens)
- Potassium voltage-gated channel subfamily kqt member 1 (76 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Human kcnq1-cam-ml277-pip2 complex in state a (Cellular component from Homo sapiens)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
human KCNQ1-CaM-ML277-PIP2 complex in state B
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7xnn
Q-score: 0.428
Ma D, Zhong L, Yan Z, Yao J, Zhang Y, Ye F, Huang Y, Lai D, Yang W, Hou P, Guo J
PNAS (2022) 119 pp. e2207067119-e2207067119 [ Pubmed: 36763058 DOI: doi:10.1073/pnas.2207067119 ]
- Human kcnq1-cam-ml277-pip2 complex in state b (Cellular component from Homo sapiens)
- (2r)-n-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-1-(4-methylbenzene-1-sulfonyl)piperidine-2-carboxamide (471 Da, Ligand)
- Calmodulin-3 (19 kDa, Protein from Homo sapiens)
- Potassium voltage-gated channel subfamily kqt member 1 (76 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
Cryo-EM structure of the PGE2-bound EP2-Gs complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7cx2
Q-score: 0.536
Qu C, Mao C, Xiao P, Shen Q, Zhong YN, Yang F, Shen DD, Tao X, Zhang H, Yan X, Zhao RJ, He J, Guan Y, Zhang C, Hou G, Zhang PJ, Hou G, Li Z, Yu X, Chai RJ, Guan YF, Sun JP, Zhang Y
Sci Adv (2021) 7 [ Pubmed: 33811074 DOI: doi:10.1126/sciadv.abf1268 ]
- Water (18 Da, Ligand)
- Nb35 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (z)-7-[(1r,2r,3r)-3-hydroxy-2-[(e,3s)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid (352 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Ep2 (Complex from Homo sapiens)
- Prostaglandin e2 receptor ep2 subtype (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pge2-bound ep2-gs complex (Complex)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Gs (Complex from Homo sapiens)